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Team:Elan Vital South Korea/l pcr
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<h1 class="title">PCR</h1> | <h1 class="title">PCR</h1> | ||
| - | <p class="listing">a. Prepare 10㎕ of cyber green, 0.5㎕ of sample DNA, 0.3㎕*(number of primer) of primer, and (9.5-0.3)*(number of primer)㎕ of distilled water</p> | + | <p class="listing">a. Prepare 10㎕ of cyber green, 0.5㎕ of sample DNA, 0.3㎕ * (number of primer) of primer, and (9.5-0.3) * (number of primer)㎕ of distilled water</p> |
<p class="listing">b .Put each of the reagents and sample DNA in each of the pcr tube, regarding the corresponding number.</p> | <p class="listing">b .Put each of the reagents and sample DNA in each of the pcr tube, regarding the corresponding number.</p> | ||
<p class="listing">c. Go through PCR.</p> | <p class="listing">c. Go through PCR.</p> | ||
Revision as of 02:41, 6 June 2014
PCR
a. Prepare 10㎕ of cyber green, 0.5㎕ of sample DNA, 0.3㎕ * (number of primer) of primer, and (9.5-0.3) * (number of primer)㎕ of distilled water
b .Put each of the reagents and sample DNA in each of the pcr tube, regarding the corresponding number.
c. Go through PCR.
d. Choose some sample according to the results of the PCR and run gel electrophoresis.
