Team:Elan Vital South Korea/l pcr

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                                <a href="https://2014hs.igem.org/Team:Elan_Vital_South_Korea/l_overview">Overview</a>
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                                <a href="https://2014hs.igem.org/Team:Elan_Vital_South_Korea/l_transformation">Transformation</a>
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                                <a href="https://2014hs.igem.org/Team:Elan_Vital_South_Korea/l_miniprep">Miniprep</a>
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                                <a href="https://2014hs.igem.org/Team:Elan_Vital_South_Korea/l_pcr">PCR</a>
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                         <h1 class="title">PCR</h1>
                         <h1 class="title">PCR</h1>
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                         <p class="listing">a. Prepare 10㎕ of cyber green, 0.5㎕ of sample DNA, 0.3㎕*(number of primer) of primer, and (9.5-0.3)*(number of primer)㎕ of distilled water</p>
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                         <p class="listing">a. Add 10μl of syber green to each PCR tube.</p>
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                         <p class="listing">b .Put each of the reagents and sample DNA in each of the pcr tube, regarding the corresponding number.</p>
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                        <p class="listing">b. Add 0.3μl of each primer to each PCR tube according to the picture.</p>
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                         <p class="listing">c. Go through PCR.</p>
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                        <p class="listing">c. Add distilled water until the mixture reaches 19.5μl.</p>
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                         <p class="listing">d. Choose some sample according to the results of the PCR and run gel electrophoresis.</p>
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                         <p class="listing">d. Add 0.5μl of sample DNA to each PCR tube according to the picture.</p>
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                         <p class="listing">e. Go through PCR.</p>
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                        <img class="wiki_img" src="https://static.igem.org/mediawiki/2014hs/f/f3/Elan_vital_process_2.png" />
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                        <p class="wiki_caption">How PCR works</p>
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                        <img class="wiki_img" src="https://static.igem.org/mediawiki/2014hs/9/96/Elan_vital_PCR_3.png" />
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                        <p class="wiki_caption">PCR machine</p>
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                        <img class="wiki_img" src="https://static.igem.org/mediawiki/2014hs/a/a6/Elan_vital_PCR_1.png" />
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                        <p class="wiki_caption">PCR program</p>
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                        <img class="wiki_img" src="https://static.igem.org/mediawiki/2014hs/b/bf/Elan_vital_PCR_2.png" />
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                        <p class="wiki_caption">PCR tube mixture</p>
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                         <p class="listing bold">7 Amp, Genta, Kana, Tetra: DNA of MRSA from patient 7 grown in ampicillin, gentamycin, kanamycin, and tetracycline, respectively.</p>
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                        <p class="listing bold">8 Amp, Genta, Kana, Tetra: DNA of MRSA from patient 8 grown in ampicillin, gentamycin, kanamycin, and tetracycline, respectively.</p>
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                        <p class="listing bold">7 AA, GA, KA, TA: DNA of E. coli grown in ampicillin, which were transformed with the DNA of MRSA from patient 7 grown in ampicillin, gentamycin, kanamycin, and tetracycline, respectively.</p>
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                        <p class="listing bold">8 GK, KK: DNA of E. coli grown in kanamycin, which were transformed with the DNA of MRSA from patient 8 grown in gentamycin and kanamycin, respectively.</p>
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                        <p class="listing bold">8 GG, KG: DNA of E. coli grown in gentamycin, which were transformed with the DNA of MRSA from patient 8 grown in gentamycin and kanamycin, respectively.</p>
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                    <p>&copy;2014 Elan Vital</p>
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Latest revision as of 13:07, 17 June 2014

PCR

a. Add 10μl of syber green to each PCR tube.

b. Add 0.3μl of each primer to each PCR tube according to the picture.

c. Add distilled water until the mixture reaches 19.5μl.

d. Add 0.5μl of sample DNA to each PCR tube according to the picture.

e. Go through PCR.

How PCR works

PCR machine

PCR program

PCR tube mixture

7 Amp, Genta, Kana, Tetra: DNA of MRSA from patient 7 grown in ampicillin, gentamycin, kanamycin, and tetracycline, respectively.

8 Amp, Genta, Kana, Tetra: DNA of MRSA from patient 8 grown in ampicillin, gentamycin, kanamycin, and tetracycline, respectively.

7 AA, GA, KA, TA: DNA of E. coli grown in ampicillin, which were transformed with the DNA of MRSA from patient 7 grown in ampicillin, gentamycin, kanamycin, and tetracycline, respectively.

8 GK, KK: DNA of E. coli grown in kanamycin, which were transformed with the DNA of MRSA from patient 8 grown in gentamycin and kanamycin, respectively.

8 GG, KG: DNA of E. coli grown in gentamycin, which were transformed with the DNA of MRSA from patient 8 grown in gentamycin and kanamycin, respectively.

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