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Team:Elan Vital South Korea/l pcr
From 2014hs.igem.org
PCR
a. Add 10μl of syber green to each PCR tube.
b. Add 0.3μl of each primer to each PCR tube according to the picture.
c. Add distilled water until the mixture reaches 19.5μl.
d. Add 0.5μl of sample DNA to each PCR tube according to the picture.
e. Go through PCR.
How PCR works
PCR machine
PCR program
PCR tube mixture
7 Amp, Genta, Kana, Tetra: DNA of MRSA from patient 7 grown in ampicillin, gentamycin, kanamycin, and tetracycline, respectively.
8 Amp, Genta, Kana, Tetra: DNA of MRSA from patient 8 grown in ampicillin, gentamycin, kanamycin, and tetracycline, respectively.
7 AA, GA, KA, TA: DNA of E. coli grown in ampicillin, which were transformed with the DNA of MRSA from patient 7 grown in ampicillin, gentamycin, kanamycin, and tetracycline, respectively.
8 GK, KK: DNA of E. coli grown in kanamycin, which were transformed with the DNA of MRSA from patient 8 grown in gentamycin and kanamycin, respectively.
8 GG, KG: DNA of E. coli grown in gentamycin, which were transformed with the DNA of MRSA from patient 8 grown in gentamycin and kanamycin, respectively.
