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| <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA">Home </a> | | <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA">Home </a> |
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| <li><a href="#">Project</a> | | <li><a href="#">Project</a> |
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| <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Project.html">Description</a></li> | | <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Project.html">Description</a></li> |
| <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Timeline">Timeline</a></li> | | <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Timeline">Timeline</a></li> |
| + | <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Lab_Successes.html">Lab Successes</a></li> |
| <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Lab_Notebook.html">Lab Notebook</a></li> | | <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Lab_Notebook.html">Lab Notebook</a></li> |
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| <p align="left"><strong>Environmental Safety</strong><br> | | <p align="left"><strong>Environmental Safety</strong><br> |
| Our project poses minimal threat to the environment. Strains like <em>E. coli</em> K12 are well-adapted to the laboratory environment, and, unlike wild type strains, have lost their ability to thrive in non-laboratory environments. Many lab strains lose their ability to form biofilms (Vidal O, Longin R, Prigent-Combaret C, Dorel C, Hooreman M, Lejeune P (1998)) meaning, if the bacteria somehow entered the environment the bacteria would not likely be able to thrive. Even so, there is always an inherent risk of releasing genetically modified organisms into the wild and for this reason proper lab decontamination procedures are always followed.</p> | | Our project poses minimal threat to the environment. Strains like <em>E. coli</em> K12 are well-adapted to the laboratory environment, and, unlike wild type strains, have lost their ability to thrive in non-laboratory environments. Many lab strains lose their ability to form biofilms (Vidal O, Longin R, Prigent-Combaret C, Dorel C, Hooreman M, Lejeune P (1998)) meaning, if the bacteria somehow entered the environment the bacteria would not likely be able to thrive. Even so, there is always an inherent risk of releasing genetically modified organisms into the wild and for this reason proper lab decontamination procedures are always followed.</p> |
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| <h2>Do any of the new BioBrick parts (or devices) that you made this year raise safety issues? If yes, did you document these issues in the Registry? How did you manage to handle the safety issue? How could other teams learn from your experience?</h2> | | <h2>Do any of the new BioBrick parts (or devices) that you made this year raise safety issues? If yes, did you document these issues in the Registry? How did you manage to handle the safety issue? How could other teams learn from your experience?</h2> |
- | <p align="left">We did not encounter any safety issues for the temperature promoter and or wintergreen enzyme generator as described in the parts registry; no other team who had previously used the same parts expressed any safety issues. Neither of these parts pose significant danger. The first part we are using is a generator of methyl salicylate—which is an organic ester naturally produced by many species of plants. Since methyl salicylate is an organic compound and it is used in many commercial fragrances, foods and, beverages, this biobrick was considered safe. The second part is a heat-activated promoter. Being a promoter, its safety is dependent on what it is promoting. As we are using it to promote the generation of methyl salicylate its use in this project did not raise any safety concerns.<br> | + | <p align="left">We did not encounter any safety issues for the temperature promoter and or wintergreen enzyme generator as described in the parts registry; no other team who had previously used the same parts expressed any safety issues. Neither of these parts pose significant danger. The first part we are using is a generator of methyl salicylate—which is an organic ester naturally produced by many species of plants. Since methyl salicylate is an organic compound and it is used in many commercial fragrances, foods and beverages, this biobrick was considered safe. The second part is a heat-activated promoter. Being a promoter, its safety is dependent on what it is promoting. As we are using it to promote the generation of methyl salicylate its use in this project did not raise any safety concerns.<br> |
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| <h2>Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project? If no, which specific biosafety rules or guidelines do you have to consider in your country?</h2></p> | | <h2>Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project? If no, which specific biosafety rules or guidelines do you have to consider in your country?</h2></p> |
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| We have moved recycling out of the lab prep area and support staff access is restricted to emptying any regular garbage that we may have. Regular lab prep for high school science labs has its own separate counter space.<br> | | We have moved recycling out of the lab prep area and support staff access is restricted to emptying any regular garbage that we may have. Regular lab prep for high school science labs has its own separate counter space.<br> |
| </p> | | </p> |
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| <h2>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices, and systems be made even safer through biosafety engineering?</h2> | | <h2>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices, and systems be made even safer through biosafety engineering?</h2> |
| + | <p> </p> |
| <p align="left">Our DIY dremelfuge is operated behind safety glass and inside of a paint can operated with a direct plug-in outside of the fume hood. iGEM teams considering utilizing this DIY plan in the future are encouraged to consider the safety requirements of such high RPM with potential for a tube to become dislodged at high speeds. DIY Incubator/shaker table risk of electrical short is minimized with fuses on the turntable power supply, breaker on the power bar, and GCFI wall plug in.</p> | | <p align="left">Our DIY dremelfuge is operated behind safety glass and inside of a paint can operated with a direct plug-in outside of the fume hood. iGEM teams considering utilizing this DIY plan in the future are encouraged to consider the safety requirements of such high RPM with potential for a tube to become dislodged at high speeds. DIY Incubator/shaker table risk of electrical short is minimized with fuses on the turntable power supply, breaker on the power bar, and GCFI wall plug in.</p> |
| <p align="left">We are concerned with the treatment of hazardous waste like that of DNA staining dyes. Although we are dealing with microliters of staining dye per gel, the disposal of such type of waste is outside the scope of our small municipality. Our team has made arrangements for proper disposal of such hazardous waste in Calgary. We would encourage other start-up high school iGEM teams to consider ahead of time their disposal needs, as it can be challenging in small communities to treat specific wastes appropriately. </p></td> | | <p align="left">We are concerned with the treatment of hazardous waste like that of DNA staining dyes. Although we are dealing with microliters of staining dye per gel, the disposal of such type of waste is outside the scope of our small municipality. Our team has made arrangements for proper disposal of such hazardous waste in Calgary. We would encourage other start-up high school iGEM teams to consider ahead of time their disposal needs, as it can be challenging in small communities to treat specific wastes appropriately. </p></td> |