Team:OLS Canmore AB CA/Lab Successes.html

From 2014hs.igem.org

(Difference between revisions)
Line 192: Line 192:
</td>
</td>
   </tr>
   </tr>
-
  <tr>
+
-
    <td><blockquote>
+
-
      <p align="left">- Successful completion of the 3A Protocols, including:<br>
+
-
      </p>
+
-
    </blockquote></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td><blockquote>
+
-
      <blockquote>
+
-
        <p align="left">- Restriction digest of plasmids<br>
+
-
          - Gel electrophoresis of plasmid digest (NB: No evidence of DNA on gel)<br>
+
-
          - Ligation protocol </p>
+
-
      </blockquote>
+
-
    </blockquote></td>
+
-
  </tr>
+
</table>
</table>
   </article>
   </article>

Revision as of 20:01, 20 June 2014

Project

 

Lab Protocol Successes - Team OLeSsence

 

  • - Mastered use of pressure-steam autoclave for all sterilization/decontamination

- Successful completion of the 3A Protocols, including:

- Restriction digest of plasmids
- Gel electrophoresis of plasmid digest (NB: No evidence of DNA on gel)
- Ligation protocol

  • - Routinely preparing our own LB-agar plates (with and without antibiotic) and LB broth for cell culture.

  • - Successful plating techniques for E. coli – routinely growing single colonies free from contamination using aseptic technique.
  • - Successfully growing cell cultures from individual colonies in DIY shaker/incubator

  • - Successfully grew E. coli cells (provided from iGEM) containing our parts (K098995 and J45119), including cell cultures.
  • - Mastered use of DIY Dremelfuge (confirming RPM with photogate) to pellet DNA
  • - Successfully mini-prepped both our parts to extract plasmid DNA

  • - Successfully used restriction digest protocols to cut our plasmids of interest, confirming presence of DNA strands of expected size via gel electrophoresis
*Need to build gel illuminator next year
  • - Successfully transformed RFP plasmid into competent cells (prepped by our mentors) to grow RFP colonies.

2014 Our Lady of the Snows iGEM