Notebook
Our documentation of activities, labs, and lectures for each day.
November 22nd, 2013
| Mission Statement
| We wanted to implement a new attendance sign-in setup, announce participation consent forms (principle consent form), and organize sub-teams.
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| Meeting Overview
| We started by electronically taking attendance and introducing the two sub-teams. All members were allowed to choose their sub-teams (may cut down R&D later). We talked about fundraising because of the December 31st deadline of registration and payment.
Possible fundraising ideas:
- Hot chocolate
- Snow-hat sales
- ShopRite Bagging
It was also the last “introductory” meeting. We announced the deadline for leadership applications due Wednesday, November 27 and reminded members to fill out consent forms.
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| Goals/Tasks for Next Meeting
| We planned to come up with an idea for fundraising and collect all the participation consent forms.
Finished principle consent form
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| Other Notes
| If members tried to look for sponsors, they had to send iGEM write-ups to them.
We found sponsors quickly and focused on small companies in Montgomery.
In addition, we checked with the supervisor to see whether he had any ideas or grants.
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December 13th, 2013
| Mission Statement
| We aimed to effectively organize the team and keep progressing with the fundraising and sponsorship efforts, as well as assemble the 2013-2014 board members.
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| Meeting Overview
| We began the meeting by announcing the 2013/2014 board members, followed by showing a video about synthetic biology to help members understand the importance of the team. The team will consist of the board as well as two sub-teams. The board collected emails and planned for fundraising and registration.
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| Goals/Tasks for Next Meeting
| We hoped to start teaching the basics of synthetic biology, organizing the ShopRite bagging fundraiser, creating online accounts for everyone, and cleaning up attendance lists.
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December 20th, 2013
| Mission Statement
| To design a biological circuit from DNA sequence.
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| Meeting Overview
| We received confirmation from ShopRite. The college advisor, Gavin from Purdue University came in to discuss synthetic biology and safety regulations. The R&D team learned how to use micropipettes while the operations discussed fundraiser ideas. .
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| Goals/Tasks for Next Meeting
| To organize and execute the Shoprite bagging fundraiser.
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January 17th, 2014
| Mission Statement
| We learned information and became organized for the future.
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| Meeting Overview
| The meeting began with a presentation on synthetic biology. We discussed cutting down the R&D team, introduced plasmids and gene cloning, and explained the operations team structure. We also researched wiki websites.
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| Goals/Tasks for Next Meeting
| There were two goals: we went over the lab procedure for the pGLO lab and we established a bank account for Montgomery iGEM.
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| Other Notes
| We created a curriculum for the next few weeks, began discussing T-shirt designs and organized a board meeting at the Princeton Public Library on Sunday, 1/19/14.
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January 24th, 2014
| Mission Statement
| Teach members about pGLO Lab.
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| Meeting Overview
| We explained how the iGEM google drive folders work and showed a bacterial transformation video. We ran a Q&A session. Each member had to demonstrate knowledge of the steps of the pGLO lab.
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| Goals/Tasks for Next Meeting
| We will understand the process of bacterial transformation and introduce laboratory techniques
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| Other Notes
| T-shirt design should be done by Feb 14th. Design winner gets free t-shirt
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January 31th, 2014
| Mission Statement
| Go over pGLO lab technique and properly inform team members of all safety procedures.
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| Meeting Overview
| Review aseptic technique and the pGLO lab.
Went over how to clean with a Bunsen Burner (if metal use plastic loop).
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| Goals/Tasks for Next Meeting
| We accomplished the pGLO lab successfully without contamination and learned to make our own plasmids to put into bacteria.
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February 3rd, 2014
| Mission Statement
| Continue preparations for pGLO Lab.
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| Meeting Overview
| We continued preparations for the pGLO lab. Members reviewed how the the lab procedure and also reviewed how the plasmid would be affected during the process.
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| Goals/Tasks for Next Meeting
| There was a complication with the materials which didn’t allow us to do the lab at the next meeting.
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February 20th, 2014
| Mission Statement
| We changed the dynamics of iGEM and administered the lab practical.
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| Meeting Overview
| We collected t-shirt money, and Robert gave a speech on dynamics. There was a Q&A session about the lab practical and iGEM
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| Goals/Tasks for Next Meeting
| Prepare for pGLO lab
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| Other Notes
| Shoprite bagging in March
Need to take yearbook photo soon.
Meetings are mandatory.
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March 7th, 2014
| Mission Statement
| We finished the pGLO lab today. We organized the operations team.
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| Meeting Overview
| We formatted team organization, finalize acne project description, and administered the practice lab documentations. Board member headshots were also taken.
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| Goals/Tasks for Next Meeting
| We started DNA ligation lessons and researched more on the acne project.
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| Other Notes
| We started website structure.
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March 14th, 2014
| Mission Statement
| We played an icebreaker to unify the team, went over lab results, and did post-lab calculations.
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| Meeting Overview
| We went over t-shirt sizes, reviewed team members' names and favorite Pokemon, went over results from pGLO lab, counted number of colonies growing under UV light, and calculated transformation efficiency.
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| Goals/Tasks for Next Meeting
| We will learn about DNA ligation.
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| Other Notes
| Although some students failed, they worked hard on the lab.
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March 14th, 2014
| Mission Statement
| We played an icebreaker to unify the team, went over lab results, and did post-lab calculations.
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| Meeting Overview
| We went over t-shirt sizes, reviewed team members' names and favorite Pokemon, went over results from pGLO lab, counted number of colonies growing under UV light, and calculated transformation efficiency.
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| Goals/Tasks for Next Meeting
| We will learn about DNA ligation.
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| Other Notes
| Lab results turned out to be rather favorable despite a few students failed attempts.
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March 21th, 2014
| Mission Statement
| Learn about DNA Ligation
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| Meeting Overview
| We received a lesson on DNA Ligation from Mr.Resch.
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April 4th, 2014
| Mission Statement
| Continue research on project.
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| Meeting Overview
| We continued researching appropriate promoters, ribosome binding sites, and terminators on the iGem registry.
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| Goals/Tasks for Next Meeting
| We will receive a lesson on mathematical modeling from Dr. Fishman.
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April 11th, 2014
| Mission Statement
| We had a presentation of mathematical modeling by Dr.Fishman, our school's AP calculus teacher
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| Meeting Overview
| Dr. Fishman came to for an overview of mathematical modeling.
R&D researched further for an enzyme that would break down fatty acids.
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| Goals/Tasks for Next Meeting
| We hope to further understand mathematical modeling.
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May 30th, 2014
| Mission Statement
| Refine the Team Wiki and order the materials for our project.
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| Meeting Overview
| Board Members and other juniors gathered to finalize the wiki page as well as take care of materials. We all continued researching promoters and ribosome binding sites for our project.
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| Goals/Tasks for Next Meeting
| Continue progressing through the steps needed to finalize the project and synthesize our desired protein.
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June 14th, 2014
| Mission Statement
| To make as much progress on the wiki as well as the poster.
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| Meeting Overview
| Board Members worked on adding information and editing the wiki. We also streaked bacteria onto agar plates to grow so that we will have more of the plasmids inside them. We were also able to continue to add content to the poster.
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| Goals/Tasks for Next Meeting
| Continue to finalize the wiki and poster.
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