- Mastered use of pressure-steam autoclave for all sterilization/decontamination
- Successful completion of the 3A Protocols, including:
- Restriction digest of plasmids
- Gel electrophoresis of plasmid digest (NB: No evidence of DNA on gel)
- Ligation protocol
- Routinely preparing our own LB-agar plates (with and without antibiotic) and LB broth for cell culture.
- Successful plating techniques for E. coli – routinely growing single colonies free from contamination using aseptic technique.
- Successfully growing cell cultures from individual colonies in DIY shaker/incubator
- Successfully grew E. coli cells (provided from iGEM) containing our parts (K098995 and J45119), including cell cultures.
- Mastered use of DIY Dremelfuge (confirming RPM with photogate) to pellet DNA
- Successfully mini-prepped both our parts to extract plasmid DNA
- Successfully used restriction digest protocols to cut our plasmids of interest, confirming presence of DNA strands of expected size via gel electrophoresis
*Need to build gel illuminator next year
- Successfully transformed RFP plasmid into competent cells (prepped by our mentors) to grow RFP colonies.
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