Error Analysis

As a first year team, we have run into many problems throughout the course of the season from financial hardship to laboratory mistakes. Here we share our experiences throughout the year in hopes that other teams can learn from our mistakes and have an easier time through the season.

Administrative Problems

Because we are in the nascent period of the STEM program, we did not receive adequate pecuniary support from our school district. In order to earn money to purchase the registry parts and lab equipment, we reached out to our local supermarkets and stores. As we informed our community of our iGEM project, we asked for their donations to contribute to our cause. One way we earned money was through our Shoprite fundraiser. Members of Montgomery iGEM volunteered their time in two hour shifts and helped bag for our local Shoprite. However, our mistake lay in our failure to find a corporate sponsor or similar sponsor. The support would have given us much more time to worry about the scientific side of iGEM and allowed us to purchase more advanced equipment and accommodate for multiple reactions.

Laboratory Issues

Growing Cultures

In our first attempt to culture the bacteria, we made the mistake of trying to culture the bacteria on a Ampicillin and Kanamycin LB Agar plate. We were following the protocol from the 3A assembly but forgot that the parts we ordered were not exactly identical to the parts in the distribution. We waited two or three days for the bacteria to grow before we began analyzing what we had done wrong (since expected growth should happen within 24 hours of inoculation). Upon closer analysis, we realized that the parts we had streaked were not antibiotic resistant and repeated the inoculation on plan LB Agar plates. Two days later, we had our bacteria.

Contamination

We also need to take into account the contamination that occurs during lab procedures. Although we follow all safety cautions to maintain accuracy of our lab results and keep ourselves safe from the deleterious chemicals, the environment where we perform our lab experiments is not as pristine as a professional laboratory, so errors due to contamination were unavoidable. Since our facility is seldom open over the weekends, our competent bacteria grew extremely rapidly throughout the days we were absent. When we came back to check on the growth, there were various samples of mold inside the plates. This demonstrates the fact that the plates were initially contaminated. We bleached these out for disposal and were more careful in our future endeavors.