Team:Montgomery Cougars NJUSA/Team Log
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{{Montgomery_Cougars_NJUSA/Layout:Two_Column}} | {{Montgomery_Cougars_NJUSA/Layout:Two_Column}} | ||
+ | =Team Log= | ||
+ | The Montgomery iGEM's teams documentation of activities, labs, and lectures for each day. | ||
+ | {{anchor|22nov2013}} | ||
{| class="wikitable" style="text-align:left; width:100%; height:200px;" | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
|- | |- | ||
- | !colspan="2" style="background: #ffdead;" | | + | !colspan="2" style="background: #ffdead;" |November 22nd 2013 |
|- | |- | ||
! width="140" | Mission Statement | ! width="140" | Mission Statement | ||
- | | | + | | We wanted to implement a new attendance sign-in setup, announce participation consent forms (principle consent form), and organize sub-teams. |
|- | |- | ||
! width="140" |Meeting Overview | ! width="140" |Meeting Overview | ||
- | | We | + | | We started by electronically taking attendance and introducing the two sub-teams. All members were allowed to choose their sub-teams (may cut down R&D later). We talked about fundraising because of the December 31st deadline of registration and payment. |
+ | Possible fundraising ideas: | ||
+ | - Hot chocolate | ||
+ | - Snow-hat sales | ||
+ | - ShopRite Bagging | ||
+ | It was also the last “introductory” meeting. We announced the deadline for leadership applications due Wednesday, November 27 and reminded members to fill out consent forms. | ||
|- | |- | ||
! width="140" |Goals/Tasks for Next Meeting | ! width="140" |Goals/Tasks for Next Meeting | ||
- | | We | + | | We planned to come up with an idea for fundraising and collect all the participation consent forms. |
+ | |||
+ | Finished principle consent form | ||
+ | |- | ||
+ | ! width="140" |Other Notes | ||
+ | | If members tried to look for sponsors, they had to send iGEM write-ups to them. | ||
+ | We found sponsors quickly and focused on small companies in Montgomery. | ||
+ | In addition, we checked with the supervisor to see whether he had any ideas or grants. | ||
|- | |- | ||
|} | |} | ||
+ | {{anchor|13dec2013}} | ||
{| class="wikitable" style="text-align:left; width:100%; height:200px;" | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
|- | |- | ||
- | !colspan="2" style="background: #ffdead;" | | + | !colspan="2" style="background: #ffdead;" |December 13th 2013 |
|- | |- | ||
! width="140" | Mission Statement | ! width="140" | Mission Statement | ||
- | | | + | |We aimed to effectively organize the team and keep progressing with the fundraising and sponsorship efforts, as well as assemble the 2013-2014 board members. |
|- | |- | ||
! width="140" |Meeting Overview | ! width="140" |Meeting Overview | ||
- | | | + | | We began the meeting by announcing the 2013/2014 board members, followed by showing a video about synthetic biology to help members understand the importance of the team. The team will consist of the board as well as two sub-teams. The board collected emails and planned for fundraising and registration. |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
|- | |- | ||
! width="140" |Goals/Tasks for Next Meeting | ! width="140" |Goals/Tasks for Next Meeting | ||
- | | | + | | We hoped to start teaching the basics of synthetic biology, organizing the ShopRite bagging fundraiser, creating online accounts for everyone, and cleaning up attendance lists. |
- | + | ||
- | + | ||
- | + | ||
|- | |- | ||
- | + | |} | |
- | + | ||
- | + | {{anchor|20dec2013}} | |
- | + | ||
- | + | ||
- | + | ||
- | |} | + | |
{| class="wikitable" style="text-align:left; width:100%; height:200px;" | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
|- | |- | ||
Line 50: | Line 53: | ||
|- | |- | ||
! width="140" |Mission Statement | ! width="140" |Mission Statement | ||
- | | | + | |We designed biological circuit from DNA sequence |
|- | |- | ||
! width="140" |Meeting Overview | ! width="140" |Meeting Overview | ||
Line 59: | Line 62: | ||
|- | |- | ||
|} | |} | ||
+ | |||
+ | {{anchor|17jan2014}} | ||
{| class="wikitable" style="text-align:left; width:100%; height:200px;" | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
|- | |- | ||
- | !colspan="2" style="background: #ffdead;" |January 17th | + | !colspan="2" style="background: #ffdead;" |January 17th 2014 |
|- | |- | ||
! width="140" | Mission Statement | ! width="140" | Mission Statement | ||
- | | | + | | We learned information and became organized for the future. |
|- | |- | ||
! width="140" |Meeting Overview | ! width="140" |Meeting Overview | ||
Line 77: | Line 82: | ||
|} | |} | ||
+ | |||
+ | {{anchor|24jan2014}} | ||
{| class="wikitable" style="text-align:left; width:100%; height:200px;" | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
|- | |- | ||
Line 95: | Line 102: | ||
|} | |} | ||
+ | {{anchor|31jan2014}} | ||
{| class="wikitable" style="text-align:left; width:100%; height:200px;" | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
|- | |- | ||
Line 100: | Line 108: | ||
|- | |- | ||
! width="140" | Mission Statement | ! width="140" | Mission Statement | ||
- | | | + | | Go over lab technique. |
|- | |- | ||
! width="140" |Meeting Overview | ! width="140" |Meeting Overview | ||
- | | | + | | Aseptic technique, review pGLO lab. |
- | + | ||
- | How to clean with a Bunsen Burner(if metal use plastic loop. | + | How to clean with a Bunsen Burner(if metal use plastic loop). |
|- | |- | ||
! width="140" |Goals/Tasks for Next Meeting | ! width="140" |Goals/Tasks for Next Meeting | ||
- | | | + | | We accomplished the pGLO lab successfully without contamination and learned to make our own plasmids to put into bacteria. |
+ | |- | ||
+ | |} | ||
+ | |||
+ | {{anchor|3feb2014}} | ||
+ | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
+ | |- | ||
+ | !colspan="2" style="background: #ffdead;" | February 3rd 2014 | ||
+ | |- | ||
+ | ! width="140" | Mission Statement | ||
+ | | Continue preparations for pGLO Lab. | ||
+ | |- | ||
+ | ! width="140" |Meeting Overview | ||
+ | | We continued preparations for the pGLO lab. Members reviewed how the the lab procedure and also reviewed how the plasmid would be affected during the process. | ||
+ | |- | ||
+ | ! width="140" |Goals/Tasks for Next Meeting | ||
+ | | There was a complication with the materials which didn’t allow us to do the lab at the next meeting. | ||
+ | |- | ||
+ | |} | ||
+ | |||
+ | {{anchor|20feb2014}} | ||
+ | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
+ | |- | ||
+ | !colspan="2" style="background: #ffdead;" |February 20th 2014 | ||
+ | |- | ||
+ | ! width="140" | Mission Statement | ||
+ | | We changed the dynamics of iGEM and administered the lab practical. | ||
+ | |- | ||
+ | ! width="140" |Meeting Overview | ||
+ | | We collected t-shirt money, and Robert gave a speech on dynamics. There was a Q&A session about the lab practical and iGEM | ||
+ | |||
+ | |- | ||
+ | ! width="140" |Goals/Tasks for Next Meeting | ||
+ | | Prepare for pGLO lab | ||
|- | |- | ||
! width="140" |Other Notes | ! width="140" |Other Notes | ||
- | | | + | | Shoprite bagging in March |
+ | Need to take yearbook photo soon. | ||
+ | Meetings are mandatory. | ||
+ | |- | ||
+ | |} | ||
+ | |||
+ | {{anchor|7mar2014}} | ||
+ | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
+ | |- | ||
+ | !colspan="2" style="background: #ffdead;" |March 7th 2014 | ||
+ | |- | ||
+ | ! width="140" | Mission Statement | ||
+ | | We finished the pGLO lab today. We organized the operations team. | ||
+ | |- | ||
+ | ! width="140" |Meeting Overview | ||
+ | | We formatted team organization, finalize acne project description, and administered the practice lab documentations. Board member headshots were also taken. | ||
+ | |||
+ | |- | ||
+ | ! width="140" |Goals/Tasks for Next Meeting | ||
+ | | We started DNA ligation lessons and researched more on the acne project. | ||
+ | |- | ||
+ | ! width="140" |Other Notes | ||
+ | | We started website structure. | ||
+ | |- | ||
+ | |} | ||
+ | |||
+ | {{anchor|14mar2014}} | ||
+ | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
+ | |- | ||
+ | !colspan="2" style="background: #ffdead;" |March 14th 2014 | ||
+ | |- | ||
+ | ! width="140" | Mission Statement | ||
+ | | We played an icebreaker to unify the team, went over lab results, and did post-lab calculations. | ||
+ | |- | ||
+ | ! width="140" |Meeting Overview | ||
+ | | We went over t-shirt sizes, reviewed team members' names and favorite Pokemon, went over results from pGLO lab, counted number of colonies growing under UV light, and calculated transformation efficiency. | ||
+ | |||
+ | |- | ||
+ | ! width="140" |Goals/Tasks for Next Meeting | ||
+ | | We will learn about DNA ligation. | ||
+ | |- | ||
+ | ! width="140" |Other Notes | ||
+ | | Although some students failed, they worked hard on the lab. | ||
+ | |- | ||
+ | |} | ||
+ | |||
+ | |||
+ | {{anchor|14mar2014}} | ||
+ | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
+ | |- | ||
+ | !colspan="2" style="background: #ffdead;" |March 14th 2014 | ||
+ | |- | ||
+ | ! width="140" | Mission Statement | ||
+ | | We played an icebreaker to unify the team, went over lab results, and did post-lab calculations. | ||
+ | |- | ||
+ | ! width="140" |Meeting Overview | ||
+ | | We went over t-shirt sizes, reviewed team members' names and favorite Pokemon, went over results from pGLO lab, counted number of colonies growing under UV light, and calculated transformation efficiency. | ||
+ | |||
+ | |- | ||
+ | ! width="140" |Goals/Tasks for Next Meeting | ||
+ | | We will learn about DNA ligation. | ||
+ | |- | ||
+ | ! width="140" |Other Notes | ||
+ | | Although some students failed, they worked hard on the lab. | ||
+ | |- | ||
+ | |} | ||
+ | |||
+ | {{anchor|21mar2014}} | ||
+ | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
+ | |- | ||
+ | !colspan="2" style="background: #ffdead;" |March 21th 2014 | ||
+ | |- | ||
+ | ! width="140" | Mission Statement | ||
+ | | Learn about DNA Ligation | ||
+ | |- | ||
+ | ! width="140" |Meeting Overview | ||
+ | | We received a lesson on DNA Ligation from Mr.Resch. | ||
+ | |- | ||
+ | |} | ||
+ | |||
+ | {{anchor|4apr2014}} | ||
+ | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
+ | |- | ||
+ | !colspan="2" style="background: #ffdead;" |April 4th 2014 | ||
+ | |- | ||
+ | ! width="140" | Mission Statement | ||
+ | | Continue research on project. | ||
+ | |- | ||
+ | ! width="140" |Meeting Overview | ||
+ | | We continued researching appropriate promoters, ribosome binding sites, and terminators on the iGem registry. | ||
+ | |- | ||
+ | ! width="140" |Goals/Tasks for Next Meeting | ||
+ | | We will receive a lesson on mathematical modeling from Dr. Fishman. | ||
+ | |- | ||
+ | |} | ||
+ | |||
+ | {{anchor|11apr2014}} | ||
+ | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
+ | |- | ||
+ | !colspan="2" style="background: #ffdead;" |April 11th 2014 | ||
+ | |- | ||
+ | ! width="140" | Mission Statement | ||
+ | | We had a presentation of mathematical modeling by Dr.Fishman. | ||
+ | |- | ||
+ | ! width="140" |Meeting Overview | ||
+ | | Dr. Fishman came to for an overview of mathematical modeling. | ||
+ | R&D researched further for an enzyme that would break down fatty acids. | ||
+ | |||
+ | |- | ||
+ | ! width="140" |Goals/Tasks for Next Meeting | ||
+ | | We hope to further understand mathematical modeling. | ||
+ | |- | ||
+ | |} | ||
+ | |||
+ | {{anchor|30may2014}} | ||
+ | {| class="wikitable" style="text-align:left; width:100%; height:200px;" | ||
+ | |- | ||
+ | !colspan="2" style="background: #ffdead;" |May 30th 2014 | ||
+ | |- | ||
+ | ! width="140" | Mission Statement | ||
+ | | Refine the Team Wiki and order the materials for our project. | ||
+ | |- | ||
+ | ! width="140" |Meeting Overview | ||
+ | | Board Members and other juniors gathered to finalize the wiki page as well as take care of materials. We all continued researching promoters and ribosome binding sites for our project. | ||
+ | |- | ||
+ | ! width="140" |Goals/Tasks for Next Meeting | ||
+ | | Continue progressing through the steps needed to finalize the project and synthesize our desired protein. | ||
|- | |- | ||
|} | |} |
Latest revision as of 19:55, 3 June 2014
Team Log
The Montgomery iGEM's teams documentation of activities, labs, and lectures for each day.
November 22nd 2013 | |
---|---|
Mission Statement | We wanted to implement a new attendance sign-in setup, announce participation consent forms (principle consent form), and organize sub-teams. |
Meeting Overview | We started by electronically taking attendance and introducing the two sub-teams. All members were allowed to choose their sub-teams (may cut down R&D later). We talked about fundraising because of the December 31st deadline of registration and payment.
Possible fundraising ideas: - Hot chocolate - Snow-hat sales - ShopRite Bagging It was also the last “introductory” meeting. We announced the deadline for leadership applications due Wednesday, November 27 and reminded members to fill out consent forms. |
Goals/Tasks for Next Meeting | We planned to come up with an idea for fundraising and collect all the participation consent forms.
Finished principle consent form |
Other Notes | If members tried to look for sponsors, they had to send iGEM write-ups to them.
We found sponsors quickly and focused on small companies in Montgomery. In addition, we checked with the supervisor to see whether he had any ideas or grants. |
December 13th 2013 | |
---|---|
Mission Statement | We aimed to effectively organize the team and keep progressing with the fundraising and sponsorship efforts, as well as assemble the 2013-2014 board members. |
Meeting Overview | We began the meeting by announcing the 2013/2014 board members, followed by showing a video about synthetic biology to help members understand the importance of the team. The team will consist of the board as well as two sub-teams. The board collected emails and planned for fundraising and registration. |
Goals/Tasks for Next Meeting | We hoped to start teaching the basics of synthetic biology, organizing the ShopRite bagging fundraiser, creating online accounts for everyone, and cleaning up attendance lists. |
December 20th 2013 | |
---|---|
Mission Statement | We designed biological circuit from DNA sequence |
Meeting Overview | We received confirmation from ShopRite. The college advisor, Gavin from Purdue University came in to discuss synthetic biology and safety regulations. The R&D team learned how to use micropipettes while the operations discussed fundraiser ideas. . |
Goals/Tasks for Next Meeting | ShopRite bagging fundraiser |
January 17th 2014 | |
---|---|
Mission Statement | We learned information and became organized for the future. |
Meeting Overview | The meeting began with a presentation on synthetic biology. We discussed cutting down the R&D team, introduced plasmids and gene cloning, and explained the operations team structure. We also researched wiki websites. |
Goals/Tasks for Next Meeting | There were two goals: we went over the lab procedure for the pGLO lab and we established a bank account for Montgomery iGEM. |
Other Notes | We created a curriculum for the next few weeks, began discussing T-shirt designs and organized a board meeting at the Princeton Public Library on Sunday, 1/19/14. |
January 24th 2014 | |
---|---|
Mission Statement | Teach members about pGLO Lab. |
Meeting Overview | We explained how the iGEM google drive folders work and showed a bacterial transformation video. We ran a Q&A session. Each member had to demonstrate knowledge of the steps of the pGLO lab. |
Goals/Tasks for Next Meeting | We will understand the process of bacterial transformation and introduce laboratory techniques |
Other Notes | T-shirt design should be done by Feb 14th. Design winner gets free t-shirt |
January 31th 2014 | |
---|---|
Mission Statement | Go over lab technique. |
Meeting Overview | Aseptic technique, review pGLO lab.
How to clean with a Bunsen Burner(if metal use plastic loop). |
Goals/Tasks for Next Meeting | We accomplished the pGLO lab successfully without contamination and learned to make our own plasmids to put into bacteria. |
February 3rd 2014 | |
---|---|
Mission Statement | Continue preparations for pGLO Lab. |
Meeting Overview | We continued preparations for the pGLO lab. Members reviewed how the the lab procedure and also reviewed how the plasmid would be affected during the process. |
Goals/Tasks for Next Meeting | There was a complication with the materials which didn’t allow us to do the lab at the next meeting. |
February 20th 2014 | |
---|---|
Mission Statement | We changed the dynamics of iGEM and administered the lab practical. |
Meeting Overview | We collected t-shirt money, and Robert gave a speech on dynamics. There was a Q&A session about the lab practical and iGEM |
Goals/Tasks for Next Meeting | Prepare for pGLO lab |
Other Notes | Shoprite bagging in March
Need to take yearbook photo soon. Meetings are mandatory. |
March 7th 2014 | |
---|---|
Mission Statement | We finished the pGLO lab today. We organized the operations team. |
Meeting Overview | We formatted team organization, finalize acne project description, and administered the practice lab documentations. Board member headshots were also taken. |
Goals/Tasks for Next Meeting | We started DNA ligation lessons and researched more on the acne project. |
Other Notes | We started website structure. |
March 14th 2014 | |
---|---|
Mission Statement | We played an icebreaker to unify the team, went over lab results, and did post-lab calculations. |
Meeting Overview | We went over t-shirt sizes, reviewed team members' names and favorite Pokemon, went over results from pGLO lab, counted number of colonies growing under UV light, and calculated transformation efficiency. |
Goals/Tasks for Next Meeting | We will learn about DNA ligation. |
Other Notes | Although some students failed, they worked hard on the lab. |
March 14th 2014 | |
---|---|
Mission Statement | We played an icebreaker to unify the team, went over lab results, and did post-lab calculations. |
Meeting Overview | We went over t-shirt sizes, reviewed team members' names and favorite Pokemon, went over results from pGLO lab, counted number of colonies growing under UV light, and calculated transformation efficiency. |
Goals/Tasks for Next Meeting | We will learn about DNA ligation. |
Other Notes | Although some students failed, they worked hard on the lab. |
March 21th 2014 | |
---|---|
Mission Statement | Learn about DNA Ligation |
Meeting Overview | We received a lesson on DNA Ligation from Mr.Resch. |
April 4th 2014 | |
---|---|
Mission Statement | Continue research on project. |
Meeting Overview | We continued researching appropriate promoters, ribosome binding sites, and terminators on the iGem registry. |
Goals/Tasks for Next Meeting | We will receive a lesson on mathematical modeling from Dr. Fishman. |
April 11th 2014 | |
---|---|
Mission Statement | We had a presentation of mathematical modeling by Dr.Fishman. |
Meeting Overview | Dr. Fishman came to for an overview of mathematical modeling.
R&D researched further for an enzyme that would break down fatty acids. |
Goals/Tasks for Next Meeting | We hope to further understand mathematical modeling. |
May 30th 2014 | |
---|---|
Mission Statement | Refine the Team Wiki and order the materials for our project. |
Meeting Overview | Board Members and other juniors gathered to finalize the wiki page as well as take care of materials. We all continued researching promoters and ribosome binding sites for our project. |
Goals/Tasks for Next Meeting | Continue progressing through the steps needed to finalize the project and synthesize our desired protein. |
Our Project
- Project Overview
- Pathogenesis of Acne
- Selecting the Gene
- Registry Parts
- Mathematical Model
- Error Analysis