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From 2014hs.igem.org

1.)Determine if the PhO sensor is the one that we want to use. 2.)Find the yeast plasmid vector to put the sequence into 3.)Determine the restriction enzymes needed to cut the plasmid and put the sequence in. 4.)Get the red fluorescent protein (RFP) DNA sequence 5.)Find and determine if the invertor DNA sequence will work 6.)Gibson Assembly 7.)Put plasmid through PCR 8.)Test with electrophoresis 9.)Cut sequences out of gels if working 10.)Inject plasmid into yeast 11.)Grow yeast 12.)Test yeast 13.)Develop standardization test for red color 14.)Water quality testing