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Team:HTHS Trussville AL/Team Log
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== August , 2013 == | == August , 2013 == | ||
| + | August 26, 2013: | ||
| + | Determine if the Pho Sensor is the one the group wants to use | ||
| + | Find the yeast plasmid vector to put the sequence into | ||
| + | Determine the restriction enzyme needed to cut the plasmid (and put the sequence in) | ||
| + | Get RFP (Red Fluorescent Protein) DNA sequence | ||
| + | Find and determine if the inverter DNA sequence will work | ||
| + | Gibson Assembly | ||
| + | Put plasmid through PCR | ||
| + | Test with electrophoresis | ||
| + | Cut sequences out of gels if working | ||
| + | Inject plasmid into yeast | ||
| + | Grow Yeast | ||
| + | Test Yeast | ||
| + | Develop standardization test for red color | ||
| + | Water quality test | ||
== September 17, 2013 == | == September 17, 2013 == | ||
Revision as of 02:51, 21 June 2014
August , 2013
August 26, 2013: Determine if the Pho Sensor is the one the group wants to use Find the yeast plasmid vector to put the sequence into Determine the restriction enzyme needed to cut the plasmid (and put the sequence in) Get RFP (Red Fluorescent Protein) DNA sequence Find and determine if the inverter DNA sequence will work Gibson Assembly Put plasmid through PCR Test with electrophoresis Cut sequences out of gels if working Inject plasmid into yeast Grow Yeast Test Yeast Develop standardization test for red color Water quality test
September 17, 2013
To Dos:
1.)Download new software (Tinkercell and GENtle)
2.)Re-do Gantt chart
3.)Redraw plasmid
4.)Start a list of potential questions and problems
5.)Finish scholarly article reviews
6.)Put DNA sequences into software
-Find Pho 5 sequence and promoter
-Find RFP sequence
-Find plasmid
--Verify origin of replication --Verify antibiotic resistance --Antibiotic vs. antifungal
7.)Discuss restriction enzymes
April 16, 2014
Ran a flash gel on the Pho5 plasmid with shaken and incubated samples.
