Team:HTHS Trussville AL/Team Log

From 2014hs.igem.org

(Difference between revisions)
(Created page with "1.)Determine if the PhO sensor is the one that we want to use. 2.)Find the yeast plasmid vector to put the sequence into 3.)Determine the restriction enzymes needed to cut the pl...")
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1.)Determine if the PhO sensor is the one that we want to use.
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To Dos:
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2.)Find the yeast plasmid vector to put the sequence into
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3.)Determine the restriction enzymes needed to cut the plasmid and put the sequence in.
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1.)Download new software (Tinkercell and GENtle)
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4.)Get the red fluorescent protein (RFP) DNA sequence
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5.)Find and determine if the invertor DNA sequence will work
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2.)Re-do Gantt chart
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6.)Gibson Assembly
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7.)Put plasmid through PCR
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3.)Redraw plasmid
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8.)Test with electrophoresis
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9.)Cut sequences out of gels if working
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4.)Start a list of potential questions and problems
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10.)Inject plasmid into yeast
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11.)Grow yeast
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5.)Finish scholarly article reviews
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12.)Test yeast
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13.)Develop standardization test for red color
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6.)Put DNA sequences into software
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14.)Water quality testing
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-Find Pho 5 sequence and promoter
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-Find RFP sequence
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-Find plasmid
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--Verify origin of replication
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--Verify antibiotic resistance
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--Antibiotic vs. antifungal
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7.)Discuss restriction enzymes

Revision as of 15:19, 5 June 2014

To Dos:

1.)Download new software (Tinkercell and GENtle)

2.)Re-do Gantt chart

3.)Redraw plasmid

4.)Start a list of potential questions and problems

5.)Finish scholarly article reviews

6.)Put DNA sequences into software

-Find Pho 5 sequence and promoter

-Find RFP sequence

-Find plasmid

--Verify origin of replication --Verify antibiotic resistance --Antibiotic vs. antifungal

7.)Discuss restriction enzymes

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