2014hs.igem.org/team:Charlottesville RS/Notebook/041114

From 2014hs.igem.org

(Difference between revisions)
(April 11th, 2014)
 
(2 intermediate revisions not shown)
Line 370: Line 370:
<ul class="DropDownMenu" id="MB1-DDM1">
<ul class="DropDownMenu" id="MB1-DDM1">
<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project"><span><span>Overall Project</span></span></a></li>
<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project"><span><span>Overall Project</span></span></a></li>
-
<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/material"><span><span>Material & Methods</span></span></a>
+
<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/material"><span><span>Material & Methods</span></span></a></li>
<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project/Applications"><span><span>Applications</span></span></a></li>
<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project/Applications"><span><span>Applications</span></span></a></li>
</ul>
</ul>
Line 376: Line 376:
</li>
</li>
<li>
<li>
-
<a href="https://2014hs.igem.org/Team:Charlottesville_RS/Parts" style="color: white">Parts<!--[if gt IE 6]><!--></a><!--<![endif]-->
+
<a href="https://2014hs.igem.org/Team:Charlottesville_RS/Safety" style="color:white">Safety<!--[if gt IE 6]><!--></a><!--<![endif]-->
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
-
<ul class="DropDownMenu" id="MB1-DDM2">
 
-
<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Parts"><span><span>Submitted Parts</span></span></a></li>
 
-
<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Parts/Characterization"><span><span>Characterization</span></span></a></li>
 
-
</ul>
 
-
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
 
-
</li>
 
<li>
<li>
<a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/Overview" style="color: white">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]-->
<a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/Overview" style="color: white">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]-->
Line 413: Line 407:
''Alli Ambrosini, Lauren Ewell''
''Alli Ambrosini, Lauren Ewell''
-
We did the transformation efficiency kit sent by iGEM, to see how well out competent cells could transform the DNA. We used 5 different concentrations of DNA- .5mL, 5mL, 10mL, 25mL, and 50mL. We definitely should see at least some red cells in the 50mL plates, and possibly none in our .5mL plates.  
+
Performed the transformation efficiencies kit sent by iGEM, to see how well out competent cells could transform the DNA. Five different concentrations of DNA- .5uL, 5uL, 10uL, 25uL, and 50uL were used. Expecting to see at least some red cells in the 50uL plates, and possibly none in our .5uL plates.  

Latest revision as of 13:59, 20 June 2014

April 11th, 2014

John Grammer

Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10




Alli Ambrosini, Lauren Ewell

Performed the transformation efficiencies kit sent by iGEM, to see how well out competent cells could transform the DNA. Five different concentrations of DNA- .5uL, 5uL, 10uL, 25uL, and 50uL were used. Expecting to see at least some red cells in the 50uL plates, and possibly none in our .5uL plates.




Becky Wilbur

Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20.




Becky Wilbur

Plated transformed cells from transformation efficiency kit. Followed step #7 on page 29.

[Back]