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From 2014hs.igem.org

Lambert iGEM NoteBook

Monthly tasks

Fall 2013: Our team visited a local farm to determine needs in our community. From the visit, we began formulating the idea of a fruit preservative.

November and December 2013: We began to work on sterile techniques and safety training. Also began research into Chitin to Chitosan Pathway as well as applications of chitosan.

January 2014: Began research into source organisms for the Chitin Deacytelase gene.

February 2014: iGEM kit arrived.

March 2014: Began extracting genomic yeast DNA. Used a protocol from Looke Article, Biotechnology Volume 50. using lithium acetate etc. Results were inconsistent and yields were low.

April 2014: Used Pierce Thermoscientific Yeast DNA Extraction Kit to extract genomic DNA from S.cerevisiae. PCR using pMAL primers. Began Ligations.

May 2014: Continued ligations using Quick Ligation, Overnight ligation and T4ligase. Further research determined low efficiency of blunt ended ligations.

June 2014: Began Biobricking CDA gene. Designed Biobrick primers for CDA, PCR, digested, ligated with PSB1C3 and transformations with DH10beta competent cells.

Lab Notebook/Protocols

Visit our laboratory notebook!

Our labwork committee worked at the Lambert High School lab and also at the Styczynski lab at the Georgia Institute of Technology. The notes and protocols are all located in the link!