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Revision as of 13:16, 14 April 2014

April 11th, 2014

John Grammer

Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10

Alli Ambrosini, Lauren Ewell

We did the transformation efficiencies kit sent by iGem, to see how well out competent cells could transform the DNA. We used 5 different concentrations of DNA- .5mL, 5mL, 10mL, 25mL, and 50mL. We definitely should see at least some red cells in the 50mL plates, and possibly none in our .5mL plates.

Becky Wilbur

Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20.

Becky Wilbur

Plated transformed cells from transformation efficiency kit. Fellowed step #7 on page 29.