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Team:Lambert GA/Project
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| + | <html> | ||
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| + | <body> | ||
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| + | <h1>Our Project</h1> | ||
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| + | <h2>Project Calendar:</h2> | ||
| + | <p> | ||
| + | <td><img src="https://static.igem.org/mediawiki/2014hs/1/15/Calendar.png" style="display:block; margin-left:auto; margin-right:auto;"> | ||
| + | </p> | ||
| + | |||
| + | <h2>How we got our idea...</h2> | ||
| + | <p> | ||
| + | <td><img src="https://static.igem.org/mediawiki/2014hs/1/10/LambertSlide1.PNG" style="display:block; margin-left:auto; margin-right:auto;"> | ||
| + | </p> | ||
| + | |||
| + | <h2>What we did in the lab...</h2> | ||
| + | <p> | ||
| + | <td><img src="https://static.igem.org/mediawiki/2014hs/8/8a/LambertSlide2.PNG" style="display:block; margin-left:auto; margin-right:auto;"> | ||
| + | </p> | ||
| + | |||
| + | <h2>CDA Methodology and Results</h2> | ||
| + | <p> | ||
| + | <h3>pMAL Protein Expression Kit</h3> | ||
| + | <td><img src="https://static.igem.org/mediawiki/2014hs/4/42/LambertSlide3.PNG" width="722" height="542" style="display:block; margin-left:auto; margin-right:auto;"> | ||
| + | <h3>Biobrick</h3> | ||
| + | <td><img src="https://static.igem.org/mediawiki/2014hs/7/79/Biobrick_Slide_1.png" width="722" height="542" style="display:block; margin-left:auto; margin-right:auto;"> | ||
| + | <td><img src="https://static.igem.org/mediawiki/2014hs/8/8f/Biobrick_Slide_2.png" width="722" height="542" style="display:block; margin-left:auto; margin-right:auto;"> | ||
| + | </p> | ||
| + | |||
| + | <h2>Assay Methodology and Results</h2> | ||
| + | <p> | ||
| + | <td><img src="https://static.igem.org/mediawiki/2014hs/7/7c/LambertSlide4.PNG" style="display:block; margin-left:auto; margin-right:auto;"> | ||
| + | <td><img src="https://static.igem.org/mediawiki/2014hs/a/ad/LambertSlide5.PNG" style="display:block; margin-left:auto; margin-right:auto;"> | ||
| + | </p> | ||
| + | <h2> References</h2> | ||
| + | <p>Anitha, A., Sowmya, S., Kumar, P. T. S., Deepthi, S., Chennazhi, K. P., Ehrlich, H., . . . Jayakumar, R. Chitin and chitosan in selected biomedical applications. Progress in Polymer Science(0). doi: http://dx.doi.org/10.1016/j.progpolymsci.2014.02.008</p> | ||
| + | <p>Christodoulidou, A., Bouriotis, V., & Thireos, G. (1996). Two sporulation-specific chitin deacetylase-encoding genes are required for the ascospore wall rigidity of Saccharomyces cerevisiae. J Biol Chem, 271(49), 31420-31425.</p> | ||
| + | <p>Christodoulidou, A., Briza, P., Ellinger, A., & Bouriotis, V. (1999). Yeast ascospore wall assembly requires two chitin deacetylase isozymes. FEBS Lett, 460(2), 275-279. </p> | ||
| + | <p>Christodoulidou, A., Briza, P., Ellinger, A., & Bouriotis, V. (1999). Yeast ascospore wall assembly requires two chitin deacetylase isozymes. FEBS Lett, 460(2), 275-279. doi: http://dx.doi.org/10.1016/S0014-5793(99)01334-4</p> | ||
| + | <p>Jarmila, V., & Vavrikova, E. (2011). Chitosan derivatives with antimicrobial, antitumour and antioxidant activities--a review. Curr Pharm Des, 17(32), 3596-3607.</p> | ||
| + | <p>Liu, N., Chen, X.-G., Park, H.-J., Liu, C.-G., Liu, C.-S., Meng, X.-H., & Yu, L.-J. (2006). Effect of MW and concentration of chitosan on antibacterial activity of Escherichia coli. Carbohydrate Polymers, 64(1), 60-65. doi: http://dx.doi.org/10.1016/j.carbpol.2005.10.028</p> | ||
| + | <p>Martínez, J. P., Falomir, M. P., & Gozalbo, D. (2001). Chitin: A Structural Biopolysaccharide eLS: John Wiley & Sons, Ltd.</p> | ||
| + | <p>Martinou, A., Koutsioulis, D., & Bouriotis, V. (2003). Cloning and expression of a chitin deacetylase gene (CDA2) from Saccharomyces cerevisiae in Escherichia coli: Purification and characterization of the cobalt-dependent recombinant enzyme. Enzyme and Microbial Technology, 32(6), 757-763. doi: http://dx.doi.org/10.1016/S0141-0229(03)00048-6</p> | ||
| + | <p>Moussa, S. H., Tayel, A. A., & Al-Turki, A. I. (2013). Evaluation of fungal chitosan as a biocontrol and antibacterial agent using fluorescence-labeling. International Journal of Biological Macromolecules, 54(0), 204-208. doi:http://dx.doi.org/10.1016/j.ijbiomac.2012.12.029</p> | ||
| + | <p>Tsigos, I., Martinou, A., Kafetzopoulos, D., & Bouriotis, V. (2000). Chitin deacetylases: new, versatile tools in biotechnology. Trends in Biotechnology, 18(7), 305-312. doi: http://dx.doi.org/10.1016/S0167-7799(00)01462-1</p> | ||
| + | <p>Zakrzewska, A., Boorsma, A., Brul, S., Hellingwerf, K. J., & Klis, F. M. (2005). Transcriptional response of Saccharomyces cerevisiae to the plasma membrane-perturbing compound chitosan. Eukaryot Cell, 4(4), 703-715. doi: 10.1128/ec.4.4.703-715.2005</p> | ||
| + | <p>Zhang, H., Li, R., & Liu, W. (2011). Effects of Chitin and Its Derivative Chitosan on Postharvest Decay of Fruits: A Review. International Journal of Molecular Sciences, 12(2), 917-934.</p> | ||
| + | <p> (Anitha et al.; Christodoulidou, Bouriotis, & Thireos, 1996; A. Christodoulidou, P. Briza, A. Ellinger, & V. Bouriotis, 1999; Anna Christodoulidou, Peter Briza, Adi Ellinger, & Vassilis Bouriotis, 1999; Jarmila & Vavrikova, 2011; Liu et al., 2006; Martínez, Falomir, & Gozalbo, 2001; Martinou, Koutsioulis, & Bouriotis, 2003; Moussa, Tayel, & Al-Turki, 2013; Tsigos, Martinou, Kafetzopoulos, & Bouriotis, 2000; Zakrzewska, Boorsma, Brul, Hellingwerf, & Klis, 2005; Zhang, Li, & Liu, 2011)</p> | ||
| + | <p>Lõoke M, Kristjuhan K, Kristjuhan A. Extraction of genomic DNA from yeasts for PCR-based applications.. doi:10.2144/000113672. PubMed PMID: 21548894.</p> | ||
| + | <p>El Hadrami, A.; Adam, L.R.; El Hadrami, I.; Daayf, F. Chitosan in Plant Protection. Mar. Drugs 2010, 8, 968-987.</p> | ||
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| + | |||
| + | |||
| + | <div class="clear"> | ||
| + | </div> | ||
| + | <DIV class="navigater" align="center"> | ||
| + | <UL> | ||
| + | <LI class="column" id="HOME"><p><a href="https://2014hs.igem.org/Team:Lambert_GA"><img src="https://static.igem.org/mediawiki/2014hs/5/57/Lambertlogo.png" width="100px" height="100px"></a></p></LI> | ||
| + | <LI class="column" id="PROJECT"><p><a href="https://2014hs.igem.org/Team:Lambert_GA/Project"><img src="https://static.igem.org/mediawiki/2014hs/e/e2/Peach.png" width="100px" height="100px"></a></p></LI> | ||
| + | <LI class="column" id="SPONSORS"><p><a href="https://2014hs.igem.org/Team:Lambert_GA/Sponsors"><img src="https://static.igem.org/mediawiki/2014hs/6/6d/Strawberry.png" width="100px" height="100px"></a></p></LI> | ||
| + | <LI class="column" id="TEAM"><p><a href="https://2014hs.igem.org/Team:Lambert_GA/Team"><img src="https://static.igem.org/mediawiki/2014hs/c/c3/Blackberries.png" width="100px" height="100px"></a></p></LI> | ||
| + | <LI class="column" id="SAFETY"><p><a href="https://2014hs.igem.org/Team:Lambert_GA/Safety"><img src="https://static.igem.org/mediawiki/2014hs/0/0d/Raspberries-transparent.jpg" width="100px" height="100px"></a></p></LI> | ||
| + | <LI class="column" id="HUMAN PRACTICES"><p><a href="https://2014hs.igem.org/Team:Lambert_GA/HumanPractices"><img src="https://static.igem.org/mediawiki/2014hs/3/39/Grapes.png" width="100px" height="100px"></a></p></LI> | ||
| + | <LI class="column" id="FUN"><p><a href="https://2014hs.igem.org/Team:Lambert_GA/Fun"><img src="https://static.igem.org/mediawiki/2014hs/7/7d/Apple.png" width="100px" height="100px"></a></p></LI> | ||
| + | <LI class="column" id="NOTEBOOK"><p><a href="https://2014hs.igem.org/Team:Lambert_GA/NoteBook"><img src="https://static.igem.org/mediawiki/2014hs/b/b0/Pear.png" width="100px" height="100px"></a></p></LI> | ||
| + | </UL> | ||
| + | </DIV> | ||
| + | |||
| + | </body> | ||
| + | </html> | ||
Latest revision as of 01:11, 21 June 2014
Our Project
Project Calendar:
How we got our idea...
What we did in the lab...
CDA Methodology and Results
pMAL Protein Expression Kit
Biobrick
Assay Methodology and Results
References
Anitha, A., Sowmya, S., Kumar, P. T. S., Deepthi, S., Chennazhi, K. P., Ehrlich, H., . . . Jayakumar, R. Chitin and chitosan in selected biomedical applications. Progress in Polymer Science(0). doi: http://dx.doi.org/10.1016/j.progpolymsci.2014.02.008
Christodoulidou, A., Bouriotis, V., & Thireos, G. (1996). Two sporulation-specific chitin deacetylase-encoding genes are required for the ascospore wall rigidity of Saccharomyces cerevisiae. J Biol Chem, 271(49), 31420-31425.
Christodoulidou, A., Briza, P., Ellinger, A., & Bouriotis, V. (1999). Yeast ascospore wall assembly requires two chitin deacetylase isozymes. FEBS Lett, 460(2), 275-279.
Christodoulidou, A., Briza, P., Ellinger, A., & Bouriotis, V. (1999). Yeast ascospore wall assembly requires two chitin deacetylase isozymes. FEBS Lett, 460(2), 275-279. doi: http://dx.doi.org/10.1016/S0014-5793(99)01334-4
Jarmila, V., & Vavrikova, E. (2011). Chitosan derivatives with antimicrobial, antitumour and antioxidant activities--a review. Curr Pharm Des, 17(32), 3596-3607.
Liu, N., Chen, X.-G., Park, H.-J., Liu, C.-G., Liu, C.-S., Meng, X.-H., & Yu, L.-J. (2006). Effect of MW and concentration of chitosan on antibacterial activity of Escherichia coli. Carbohydrate Polymers, 64(1), 60-65. doi: http://dx.doi.org/10.1016/j.carbpol.2005.10.028
Martínez, J. P., Falomir, M. P., & Gozalbo, D. (2001). Chitin: A Structural Biopolysaccharide eLS: John Wiley & Sons, Ltd.
Martinou, A., Koutsioulis, D., & Bouriotis, V. (2003). Cloning and expression of a chitin deacetylase gene (CDA2) from Saccharomyces cerevisiae in Escherichia coli: Purification and characterization of the cobalt-dependent recombinant enzyme. Enzyme and Microbial Technology, 32(6), 757-763. doi: http://dx.doi.org/10.1016/S0141-0229(03)00048-6
Moussa, S. H., Tayel, A. A., & Al-Turki, A. I. (2013). Evaluation of fungal chitosan as a biocontrol and antibacterial agent using fluorescence-labeling. International Journal of Biological Macromolecules, 54(0), 204-208. doi:http://dx.doi.org/10.1016/j.ijbiomac.2012.12.029
Tsigos, I., Martinou, A., Kafetzopoulos, D., & Bouriotis, V. (2000). Chitin deacetylases: new, versatile tools in biotechnology. Trends in Biotechnology, 18(7), 305-312. doi: http://dx.doi.org/10.1016/S0167-7799(00)01462-1
Zakrzewska, A., Boorsma, A., Brul, S., Hellingwerf, K. J., & Klis, F. M. (2005). Transcriptional response of Saccharomyces cerevisiae to the plasma membrane-perturbing compound chitosan. Eukaryot Cell, 4(4), 703-715. doi: 10.1128/ec.4.4.703-715.2005
Zhang, H., Li, R., & Liu, W. (2011). Effects of Chitin and Its Derivative Chitosan on Postharvest Decay of Fruits: A Review. International Journal of Molecular Sciences, 12(2), 917-934.
(Anitha et al.; Christodoulidou, Bouriotis, & Thireos, 1996; A. Christodoulidou, P. Briza, A. Ellinger, & V. Bouriotis, 1999; Anna Christodoulidou, Peter Briza, Adi Ellinger, & Vassilis Bouriotis, 1999; Jarmila & Vavrikova, 2011; Liu et al., 2006; Martínez, Falomir, & Gozalbo, 2001; Martinou, Koutsioulis, & Bouriotis, 2003; Moussa, Tayel, & Al-Turki, 2013; Tsigos, Martinou, Kafetzopoulos, & Bouriotis, 2000; Zakrzewska, Boorsma, Brul, Hellingwerf, & Klis, 2005; Zhang, Li, & Liu, 2011)
Lõoke M, Kristjuhan K, Kristjuhan A. Extraction of genomic DNA from yeasts for PCR-based applications.. doi:10.2144/000113672. PubMed PMID: 21548894.
El Hadrami, A.; Adam, L.R.; El Hadrami, I.; Daayf, F. Chitosan in Plant Protection. Mar. Drugs 2010, 8, 968-987.
