"
2014hs.igem.org/team:Charlottesville RS/Notebook/041114
From 2014hs.igem.org
(Difference between revisions)
Ingamename (Talk | contribs) (→March 11th, 2014) |
Ingamename (Talk | contribs) |
||
| (6 intermediate revisions not shown) | |||
| Line 370: | Line 370: | ||
<ul class="DropDownMenu" id="MB1-DDM1"> | <ul class="DropDownMenu" id="MB1-DDM1"> | ||
<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project"><span><span>Overall Project</span></span></a></li> | <li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project"><span><span>Overall Project</span></span></a></li> | ||
| - | <li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/material"><span><span>Material & Methods</span></span></a> | + | <li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/material"><span><span>Material & Methods</span></span></a></li> |
<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project/Applications"><span><span>Applications</span></span></a></li> | <li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project/Applications"><span><span>Applications</span></span></a></li> | ||
</ul> | </ul> | ||
| Line 376: | Line 376: | ||
</li> | </li> | ||
<li> | <li> | ||
| - | <a href="https://2014hs.igem.org/Team:Charlottesville_RS/ | + | <a href="https://2014hs.igem.org/Team:Charlottesville_RS/Safety" style="color:white">Safety<!--[if gt IE 6]><!--></a><!--<![endif]--> |
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]--> | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]--> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
<li> | <li> | ||
<a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/Overview" style="color: white">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]--> | <a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/Overview" style="color: white">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]--> | ||
| Line 402: | Line 396: | ||
</html> | </html> | ||
| - | == | + | == April 11th, 2014 == |
''John Grammer'' | ''John Grammer'' | ||
Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10 | Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10 | ||
| + | |||
| + | ---- | ||
''Alli Ambrosini, Lauren Ewell'' | ''Alli Ambrosini, Lauren Ewell'' | ||
| - | + | Performed the transformation efficiencies kit sent by iGEM, to see how well out competent cells could transform the DNA. Five different concentrations of DNA- .5uL, 5uL, 10uL, 25uL, and 50uL were used. Expecting to see at least some red cells in the 50uL plates, and possibly none in our .5uL plates. | |
| + | |||
| + | ---- | ||
| Line 419: | Line 417: | ||
Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20. | Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20. | ||
| + | |||
| + | ---- | ||
''Becky Wilbur'' | ''Becky Wilbur'' | ||
| - | Plated transformed cells from transformation efficiency kit. | + | Plated transformed cells from transformation efficiency kit. Followed step #7 on page 29. |
| + | |||
| + | [[https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/Overview Back]] | ||
Latest revision as of 13:59, 20 June 2014
April 11th, 2014
John Grammer
Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10
Alli Ambrosini, Lauren Ewell
Performed the transformation efficiencies kit sent by iGEM, to see how well out competent cells could transform the DNA. Five different concentrations of DNA- .5uL, 5uL, 10uL, 25uL, and 50uL were used. Expecting to see at least some red cells in the 50uL plates, and possibly none in our .5uL plates.
Becky Wilbur
Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20.
Becky Wilbur
Plated transformed cells from transformation efficiency kit. Followed step #7 on page 29.
[Back]
