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<br><font style="font-size:32px" color="#E7C9CF" face="Baskerville Old Face"><b>Sources</b></font>
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<br/>
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<br><br><br>
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<script type="text/javascript">
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<font style="font-size:32px" color="#E7C9CF" face="Baskerville Old Face"><b>Sources</b></font>
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<font style="font-size:16px" color="#E0BAC1" face="Baskerville Old Face"><b><br>
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<br><br>
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1.A Gel Diffusion Assay for Visualization and Quantification of Chitinase Activity<br>
-
</script>
+
Xiaohong Zou, Hiroyuki Nonogaki, and Gregory E. Welbaum<br>
-
 
+
Molecular Biotechnology, Volume 22, 2002<br>
-
 
+
-
</head><body>
+
<br>
<br>
<br>
<br>
 +
2.A pilot study of N-acetyl glucosamine, a nutritional substrate for glycosaminoglycan synthesis, in paediatric chronic inflammatory bowel disease <br>
 +
S. Salvatore, R. Heuschkel, S. Tomlin, S. E. Davies, S. Edwards, J. A. Walker-Smith, I. French and S. H. Murch <br>
 +
Alimentary Pharmacology &amp; Therapeutics Alimentary Pharmacology &amp; Therapeutics Volume 14, Issue 12, pages 1567-1579, December 2000 (Article first published online: 2 JAN 2002)<br>
<br>
<br>
-
 
-
<div id="container">
 
-
 
<br>
<br>
 +
3.Cloning and Expression of a Chitinase Gene from Sanguibacter sp. C4<br>
 +
Tao Yong, Jin Hong, Long Zhang-Fu, Zhang Li, Ding Xiu-Qiong, Tao Ke, Liu Shi-Gui<br>
 +
Acta Genetica Sinica, November 2006<br>
<br>
<br>
<br>
<br>
-
<div id="bv_Text1" style="margin:0;padding:0;position:absolute;left:400px;top:20px;width:150px;height:36px;text-align:left;z-index:0;"><font style="font-size:32px" color="#E7C9CF" face="Baskerville Old Face"><b>Sources</b></font></div><div id="bv_Text2" style="margin:0;padding:0;position:absolute;left:73px;top:147px;width:150px;height:36px;text-align:left;z-index:1;">&nbsp;</div><div id="bv_Text3" style="margin:0;padding:0;position:absolute;left:0px;top:0px;width:975px;height:2199px;background-color:#A64A5C;text-align:left;z-index:2;"><br><br>
+
4.Expression and efficient secretion of a functional chitinase from Chromobacterium violaceum in Escherichia coli<br>
 +
Marina Duarte Pinto Lobo, Fredy Davi Albuquerque Silva, Patrícia Gadelha de Castro Landim, Paloma Ribeiro da Cruz, Thaís Lima de Brito, Suelen Carneiro de Medeiros, José Tadeu Abreu Oliveira, Ilka Maria Vasconcelos, Humberto D’Muniz Pereira and Thalles Barbosa Grangeiro<br>
 +
Lobo et al. BMC Biotechnology 2013<br>
<br>
<br>
<br>
<br>
 +
5. Metabolic engineering of Escherichia coli for industrial production of glucosamine and N-acetylglucosamine<br>
 +
Ming-De Deng,David K. Severson,Alan D. Grund,Sarah L. Wassink, Richard P. Burlingame,Alan Berry,Jeffrey A. Running,Candice A. Kunesh, Linsheng Song,Thomas A. Jerrell,Reinhardt A. Rosson<br>
 +
Bio-Technical Resources, 1035 South 7th Street, Manitowoc, WI 54220, USA<br>
 +
Received 17 September 2004; accepted 8 February 2005<br>
 +
Available online 23 March 2005<br>
<br>
<br>
<br>
<br>
-
<div id="bv_Text1" style="margin:0;padding:0;position:absolute;left:400px;top:20px;width:150px;height:36px;text-align:left;z-index:0;"><br>
+
6.Molecular Cloning, Structural Analysis, and Expression in Escherichia coli of a Chitinase Gene from Enterobacter agglomerans<br>
 +
Leonid S. Chernin, Leonardo de la Fuente, Vladimir Sobolev, Shoshan Haran, Constantin E. Vorgias, Amos B. Oppenheim, Ilan Chet<br>
 +
Applied and Environmental Microbiology, Mar. 1997, p. 834-839<br>
<br>
<br>
<br>
<br>
 +
7.N-Acetylglucosamine: Production and Applications<br>
 +
Jeen-Kuan Chen , Chia-Rui Shen&nbsp; and Chao-Lin Liu<br>
 +
Marine Drugs, Received: 22 March 2010; in revised form: 19 April 2010 / Accepted: 23 April 2010 / Published: 15 September 2010<br>
<br>
<br>
<br>
<br>
-
<br><font style="font-size:32px" color="#E7C9CF" face="Baskerville Old Face"><b>Sources</b></font></div>
+
8.Optimization of glucose feeding approaches for enhanced<br>
-
 
+
glucosamine and N-acetylglucosamine production<br>
 +
by an engineered Escherichia coli<br>
 +
Xin Chen • Long Liu • Jianghua Li •Jie Liu • Guocheng Du • Jian Chen<br>
 +
Received: 19 August 2011 / Accepted: 30 September 2011 / Published online: 19 October 2011 Society for Industrial Microbiology 2011<br>
 +
<br>
 +
<br>
 +
9.Purification and Antifungal Activity of Recombinant Chitinase from Escherichia coli Carrying the Family 19 Chitinase Gene of Streptomyces sp. J-13-3<br>
 +
Yousuke Yamashita and Katsurichiro Okazaki<br>
 +
Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan<br>
 +
Biosci. Biotechnol. Biochem., 68 (10), 2193-2196, 2004<br>
 +
<br>
 +
<br>
 +
10.Role of Chitinases in Human Stomach for Chitin Digestion: AMCase in the Gastric Digestion of Chitin and Chit in Gastric Pathologies <br>
 +
Maurizio G. Paoletti, Lorenzo Norberto, Elisa Cozzarini and Salvatore Musumeci<br>
 +
Binomium Chitin-Chitinase: Recent Issues <br>
 +
<br>
 +
<br>
 +
11.The Metabolism of Exogenous N-Acetyl-D-glucosamine <br>
 +
Biochem. J. (1961) 80, 5726-O[35S]-Sulphate in the Normal Rat<br>
 +
BY A. G. LLOYD<br>
 +
Department of Biochemi8try, Univer8ity College, Newport Road, Cardiff (Received 6 February 1961)<br>
 +
<br>
 +
<br>
 +
Find a Vitamin or Supplement N - ACETYL GLUCOSAMINE <br>
 +
<u><a href="http://www.webmd.com/vitamins-supplements/ingredientmono-619-N-ACETYL+GLUCOSAMINE.aspx?activeIngredientId=619&activeIngredientName=N-ACETYL+GLUCOSAMINE " target="http://www.webmd.com/vitamins-supplements/ingredientmono-619-N-ACETYL+GLUCOSAMINE.aspx?activeIngredientId=619&activeIngredientName=N-ACETYL+GLUCOSAMINE ">&lt;http://www.webmd.com/vitamins-supplements/ingredientmono-619-N-ACETYL+GLUCOSAMINE.aspx?activeIngredientId=619&amp;activeIngredientName=N-ACETYL+GLUCOSAMINE&gt;</u> <br>
 +
</a><br>
 +
<br>
 +
Glucosamine <br>
 +
<u><a href="http://health.yahoo.net/natstandardcontent/glucosamine" target="http://health.yahoo.net/natstandardcontent/glucosamine">&lt;http://health.yahoo.net/natstandardcontent/glucosamine&gt;</u> </a><br>
 +
<br>
 +
<br>
 +
Glucosamine Clinical Trial Summaries <br>
 +
<u><a href="http://www.ultimateglucosamine.com/consumers/clinicaltrials.shtml" target="http://www.ultimateglucosamine.com/consumers/clinicaltrials.shtml">&lt;http://www.ultimateglucosamine.com/consumers/clinicaltrials.shtml&gt;</u> <br>
 +
</a><br>
 +
<br>
 +
Heres How N Acetylglucosamine May Help Crohns (by Brad Chase)<br>
 +
<u><a href="http://www.progressivehealth.com/learn-how-n-acetylglucosamine-can-help-crohns.htm" target="http://www.progressivehealth.com/learn-how-n-acetylglucosamine-can-help-crohns.htm">&lt;http://www.progressivehealth.com/learn-how-n-acetylglucosamine-can-help-crohns.htm&gt;</u><br>
 +
</a><br>
 +
<br>
 +
Insulin Resistance and Glucosamine Supplementation<br>
 +
<u><a href="http://www.ultimateglucosamine.com/consumers/insulinresistance.shtml" target="http://www.ultimateglucosamine.com/consumers/insulinresistance.shtml">&lt;http://www.ultimateglucosamine.com/consumers/insulinresistance.shtml&gt;</u><br>
 +
</a><br>
 +
<br>
 +
N-Acetyl-D-glucosamine &amp; Glucosamine AbsorptionDistribution And Metabolism <u><a href="http://www.ultimateglucosamine.com/consumers/metabolism.shtml" target="http://www.ultimateglucosamine.com/consumers/metabolism.shtml">&lt;http://www.ultimateglucosamine.com/consumers/metabolism.shtml&gt;</u> <br>
 +
</a><br>
 +
<br>
 +
N-acetylglucosaminidase, alpha<br>
 +
<u><a href="http://www.ebi.ac.uk/s4/summary/molecular?term=n-acetyl-glucosaminidase&classification=9606&tid=gSynAT5G13690" target="http://www.ebi.ac.uk/s4/summary/molecular?term=n-acetyl-glucosaminidase&classification=9606&tid=gSynAT5G13690">&lt;http://www.ebi.ac.uk/s4/summary/molecular?term=n-acetyl-glucosaminidase&amp;classification=9606&amp;tid=gSynAT5G13690&gt;</u> <br>
 +
</a><br>
 +
<br>
 +
NAGLU »&nbsp; Alpha-N-acetylglucosaminidase <br>
 +
<u><a href="http://www.nextprot.org/db/entry/NX_P54802/expression" target="http://www.nextprot.org/db/entry/NX_P54802/expression">&lt;http://www.nextprot.org/db/entry/NX_P54802/expression&gt;</u> <br>
 +
</a><br>
 +
</b></font><font style="font-size:24px" color="#E0BAC1" face="Baskerville Old Face"><b>Articles</b></font><font style="font-size:16px" color="#E0BAC1" face="Baskerville Old Face"><b><br>
 +
<br>
 +
Quorum sensing and Chromobacterium violaceum: exploitation of violacein production<br>
 +
and inhibition for the detection of N-acylhomoserine lactones<br>
 +
Kay H. McClean, Michael K. Winson, Leigh Fish, Adrian Taylor, Siri Ram Chhabra, Miguel<br>
 +
Camara, Mavis Daykin, John H. Lamb, Simon Swift, Barrie W. Bycroft, Gordon S. A. B. Stewart <br>
 +
and Paul Williams<br>
 +
Microbiology, Issue 143, Pages 3703–3711 , 1997<br>
 +
<a href="http://www.redmicrobios.com.ar/pdf/22-CV026-1.pdf" target="http://www.redmicrobios.com.ar/pdf/22-CV026-1.pdf">http://www.redmicrobios.com.ar/pdf/22-CV026-1.pdf</a><br>
 +
<br>
 +
Cloning, Sequencing, and Expression of the Chitinase Gene chiA74 from Bacillus <br>
 +
thuringiensis<br>
 +
J. Eleazar Barboza-Corona, Elizabeth Nieto-Mazzocco, [...], and Jorge E. Ibarra<br>
 +
Applied and Enviromental Microbiology, Issue 69(2), Pages 1023-1029, February 2003<br>
 +
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC143672/" target="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC143672/">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC143672/</a><br>
 +
<br>
 +
Cloning of chitinase gene from Bacillus thuringiensis <br>
 +
T R Usharani* and T K S Gowda<br>
 +
Indian Journal of Biotechnology<br>
 +
Vol 10, July 2011, pp 264-269<br>
 +
<a href="http://nopr.niscair.res.in/bitstream/123456789/12108/1/IJBT%2010%283%29%20264-269.pdf" target="http://nopr.niscair.res.in/bitstream/123456789/12108/1/IJBT%2010%283%29%20264-269.pdf">http://nopr.niscair.res.in/bitstream/123456789/12108/1/IJBT%2010%283%29%20264-269.pdf</a><br>
 +
<br>
 +
</b></font><font style="font-size:24px" color="#E0BAC1" face="Baskerville Old Face"><b>Other sources </b></font><font style="font-size:16px" color="#E0BAC1" face="Baskerville Old Face"><b><br>
 +
<br>
 +
Plasmid data sheet <br>
 +
<a href="http://www.expressys.com/pza31luc.pdf" target="http://www.expressys.com/pza31luc.pdf">http://www.expressys.com/pza31luc.pdf</a><br>
 +
<br>
 +
A biokémia és molekuláris biológia alapjai (könyv)<br>
 +
<a href="http://elte.prompt.hu/sites/default/files/tananyagok/biokemia_alapjai/BIO7_Biokemia_alapjai.pdf" target="http://elte.prompt.hu/sites/default/files/tananyagok/biokemia_alapjai/BIO7_Biokemia_alapjai.pdf">http://elte.prompt.hu/sites/default/files/tananyagok/biokemia_alapjai/BIO7_Biokemia_alapjai.pdf</a><br>
 +
<br>
 +
Plasmid map – pZA31-Luc vector<br>
 +
<a href="https://benchling.com/bhussey/vectors/MgM-pza31-luc/" target="https://benchling.com/bhussey/vectors/MgM-pza31-luc/">https://benchling.com/bhussey/vectors/MgM-pza31-luc/</a><br>
 +
<br>
 +
Bacillus thuringiensis chitinase (chi) gene, complete cds, GenBank: AF424979.1<br>
 +
<a href="http://www.ncbi.nlm.nih.gov/nuccore/16326732" target="http://www.ncbi.nlm.nih.gov/nuccore/16326732">http://www.ncbi.nlm.nih.gov/nuccore/16326732</a><br>
 +
<br>
 +
Chitinase [Bacillus thuringiensis], GenBank: AAL17867.1<br>
 +
<a href="http://www.ncbi.nlm.nih.gov/protein/16326733" target="http://www.ncbi.nlm.nih.gov/protein/16326733">http://www.ncbi.nlm.nih.gov/protein/16326733</a><br>
 +
<br>
 +
Bacillus thuringiensis chitinase (chi74) gene, complete cds, GenBank: EF197878.1<br>
 +
<a href="http://www.ncbi.nlm.nih.gov/nuccore/EF197878.1" target="http://www.ncbi.nlm.nih.gov/nuccore/EF197878.1">http://www.ncbi.nlm.nih.gov/nuccore/EF197878.1</a><br>
 +
<br>
 +
Chitinase [Bacillus thuringiensis serovar kurstaki], GenBank: ABQ65137.2<br>
 +
<a href="http://www.ncbi.nlm.nih.gov/protein/149087537?report=genbank&log$=prottop&blast_rank=9&RID=TTBWRNZD014" target="http://www.ncbi.nlm.nih.gov/protein/149087537?report=genbank&log$=prottop&blast_rank=9&RID=TTBWRNZD014">http://www.ncbi.nlm.nih.gov/protein/149087537?report=genbank&amp;log$=prottop&amp;blast_rank=9&amp;RID=TTBWRNZD014</a><br>
 +
<br>
 +
Chitinase production by Bacillus thuringiensis and Bacillus licheniformis: their potential in antifungal biocontrol.<br>
 +
<a href="http://www.ncbi.nlm.nih.gov/pubmed/22367944" target="http://www.ncbi.nlm.nih.gov/pubmed/22367944">http://www.ncbi.nlm.nih.gov/pubmed/22367944</a><br>
 +
<br>
 +
Bacterial Transformation <br>
 +
<a href="http://www.addgene.org/plasmid_protocols/bacterial_transformation/" target="http://www.addgene.org/plasmid_protocols/bacterial_transformation/">http://www.addgene.org/plasmid_protocols/bacterial_transformation/</a><br>
 +
<br>
 +
DNA Ligation<br>
 +
<a href="http://www.addgene.org/plasmid_protocols/DNA_ligation/" target="http://www.addgene.org/plasmid_protocols/DNA_ligation/">http://www.addgene.org/plasmid_protocols/DNA_ligation/</a><br>
 +
<br>
 +
Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning)<br>
 +
<a href="http://www.addgene.org/plasmid_protocols/subcloning/" target="http://www.addgene.org/plasmid_protocols/subcloning/">http://www.addgene.org/plasmid_protocols/subcloning/<br>
 +
</a><br>
 +
Restriction Digest of Plasmid DNA<br>
 +
<a href="http://www.addgene.org/plasmid_protocols/restriction_digest/" target="http://www.addgene.org/plasmid_protocols/restriction_digest/">http://www.addgene.org/plasmid_protocols/restriction_digest/</a><br>
 +
<br>
 +
Popular Plasmids<br>
 +
<a href="https://www.addgene.org/popular-plasmids/" target="https://www.addgene.org/popular-plasmids/">https://www.addgene.org/popular-plasmids/</a><br>
 +
<br>
 +
BLAST<br>
 +
<a href="http://blast.ncbi.nlm.nih.gov/Blast.cgi#149087537" target="http://blast.ncbi.nlm.nih.gov/Blast.cgi#149087537">http://blast.ncbi.nlm.nih.gov/Blast.cgi#149087537</a><br>
 +
<br>
 +
Sequence: AF424979.1 : Bacillus thuringiensis chitinase (chi) gene, complete cds.<br>
 +
<a href="http://www.ebi.ac.uk/ena/data/view/AF424979" target="http://www.ebi.ac.uk/ena/data/view/AF424979">http://www.ebi.ac.uk/ena/data/view/AF424979</a><br>
 +
<br>
 +
Coding: AAL17867.1 : Bacillus thuringiensis chitinase<br>
 +
<a href="http://www.ebi.ac.uk/ena/data/view/AAL17867" target="http://www.ebi.ac.uk/ena/data/view/AAL17867">http://www.ebi.ac.uk/ena/data/view/AAL17867</a><br>
 +
<br>
 +
NCAIM 0025 Nutrient agar<br>
 +
<a href="http://web.uni-corvinus.hu:8089/NCAIM/ncaim/XmediumDetail.jsp?MID=23" target="http://web.uni-corvinus.hu:8089/NCAIM/ncaim/XmediumDetail.jsp?MID=23">http://web.uni-corvinus.hu:8089/NCAIM/ncaim/XmediumDetail.jsp?MID=23</a><br>
 +
<br>
 +
DESIGN PCR PRIMERS<br>
 +
<a href="http://molbiol-tools.ca/PCR.htm" target="http://molbiol-tools.ca/PCR.htm">http://molbiol-tools.ca/PCR.htm</a><br>
 +
<br>
 +
Hungarian legislation<br>
 +
<a href="http://gmo.kormany.hu/hungarian-legislation" target="http://gmo.kormany.hu/hungarian-legislation">http://gmo.kormany.hu/hungarian-legislation</a><br>
 +
<br>
 +
Assembly:Standard assembly<br>
 +
<a href="http://parts.igem.org/Assembly:Standard_assembly" target="http://parts.igem.org/Assembly:Standard_assembly">http://parts.igem.org/Assembly:Standard_assembly</a><br>
 +
<br>
 +
An Introduction to BioBricks<br>
 +
<a href="http://parts.igem.org/Help:An_Introduction_to_BioBricks" target="http://parts.igem.org/Help:An_Introduction_to_BioBricks">http://parts.igem.org/Help:An_Introduction_to_BioBricks</a><br>
 +
<br>
 +
BioBrick Prefix and Suffix<br>
 +
<a href="http://parts.igem.org/Help:BioBrick_Prefix_and_Suffix" target="http://parts.igem.org/Help:BioBrick_Prefix_and_Suffix">http://parts.igem.org/Help:BioBrick_Prefix_and_Suffix<br>
 +
</a><br>
 +
Standardization<br>
 +
<a href="http://parts.igem.org/Help:Standardization" target="http://parts.igem.org/Help:Standardization">http://parts.igem.org/Help:Standardization</a><br>
 +
<br>
 +
Assembly:RBS-CDS issues<br>
 +
<a href="http://parts.igem.org/Assembly:RBS-CDS_issues" target="http://parts.igem.org/Assembly:RBS-CDS_issues">http://parts.igem.org/Assembly:RBS-CDS_issues</a><br>
 +
<br>
 +
Part:pSB1C3:Design<br>
 +
<a href="http://parts.igem.org/Part:pSB1C3:Design" target="http://parts.igem.org/Part:pSB1C3:Design">http://parts.igem.org/Part:pSB1C3:Design</a><br>
 +
<br>
 +
Prefix and Suffix<br>
 +
<a href="http://parts.igem.org/Help:Prefix-Suffix" target="http://parts.igem.org/Help:Prefix-Suffix">http://parts.igem.org/Help:Prefix-Suffix</a><br>
 +
<br>
 +
Part:BBa_J04450<br>
 +
<a href="http://parts.igem.org/Part:BBa_J04450" target="http://parts.igem.org/Part:BBa_J04450">http://parts.igem.org/Part:BBa_J04450</a><br>
 +
<br>
 +
Laboratory biosafety manual - ISBN 92 4 154650 6<br>
 +
<a href="http://www.who.int/csr/resources/publications/biosafety/Biosafety7.pdf" target="http://www.who.int/csr/resources/publications/biosafety/Biosafety7.pdf">http://www.who.int/csr/resources/publications/biosafety/Biosafety7.pdf</a><br>
 +
<br>
 +
Safety – iGEM <br>
 +
<a href="https://2014hs.igem.org/Safety" target="https://2014hs.igem.org/Safety">https://2014hs.igem.org/Safety</a><br>
 +
<br>
 +
iGEM 2013 Basic Safety Form<br>
 +
<a href="https://static.igem.org/mediawiki/2013/8/86/IGEM_2013_Basic_Safety_Form_BLANK.pdf" target="https://static.igem.org/mediawiki/2013/8/86/IGEM_2013_Basic_Safety_Form_BLANK.pdf">https://static.igem.org/mediawiki/2013/8/86/IGEM_2013_Basic_Safety_Form_BLANK.pdf</a><br>
<br>
<br>
 +
Antibiotic usage<br>
 +
<a href="https://www.k-state.edu/hermanlab/protocols/AntibioticUsage.html" target="https://www.k-state.edu/hermanlab/protocols/AntibioticUsage.html">https://www.k-state.edu/hermanlab/protocols/AntibioticUsage.html</a><br>
<br>
<br>
 +
Bacterial Genomic DNA<br>
 +
<a href="http://www.dsmz.de/services/services-microorganisms/bacterial-genomic-dna.html" target="http://www.dsmz.de/services/services-microorganisms/bacterial-genomic-dna.html">http://www.dsmz.de/services/services-microorganisms/bacterial-genomic-dna.html</a><br>
<br>
<br>
-
<br><font style="font-size:16px" color="#E0BAC1" face="Baskerville Old Face"><b>
+
Product information - ThermoScientific DreamTaq Green PCR Master Mix (2X) # K1081 200<br>
-
1.A Gel Diffusion Assay for Visualization and Quantification of Chitinase Activity<br>Xiaohong Zou, Hiroyuki Nonogaki, and Gregory E. Welbaum<br>Molecular Biotechnology, Volume 22, 2002<br><br><br>2.A pilot study of N-acetyl glucosamine, a nutritional substrate for glycosaminoglycan synthesis, in paediatric chronic inflammatory bowel disease<br>S. Salvatore, R. Heuschkel, S. Tomlin, S. E. Davies, S. Edwards, J. A. Walker-Smith, I. French and S. H. Murch<br>Alimentary Pharmacology &amp; Therapeutics Alimentary Pharmacology &amp; Therapeutics Volume 14, Issue 12, pages 1567-1579, December 2000 (Article first published online: 2 JAN 2002)<br><br><br>3.Cloning and Expression of a Chitinase Gene from Sanguibacter sp. C4<br>Tao Yong, Jin Hong, Long Zhang-Fu, Zhang Li, Ding Xiu-Qiong, Tao Ke, Liu Shi-Gui<br>Acta Genetica Sinica, November 2006<br><br><br>4.Expression and efficient secretion of a functional chitinase from Chromobacterium violaceum in Escherichia coli<br>Marina Duarte Pinto Lobo, Fredy Davi Albuquerque Silva, Patrícia Gadelha de Castro Landim, Paloma Ribeiro da Cruz, Thaís Lima de Brito, Suelen Carneiro de Medeiros, José Tadeu Abreu Oliveira, Ilka Maria Vasconcelos, Humberto D’Muniz Pereira and Thalles Barbosa Grangeiro<br>Lobo et al. BMC Biotechnology 2013<br><br><br>5. Metabolic engineering of Escherichia coli for industrial production of glucosamine and N-acetylglucosamine<br>Ming-De Deng,David K. Severson,Alan D. Grund,Sarah L. Wassink, Richard P. Burlingame,Alan Berry,Jeffrey A. Running,Candice A. Kunesh, Linsheng Song,Thomas A. Jerrell,Reinhardt A. Rosson<br>Bio-Technical Resources, 1035 South 7th Street, Manitowoc, WI 54220, USA<br>Received 17 September 2004; accepted 8 February 2005<br>Available online 23 March 2005<br><br><br>6.Molecular Cloning, Structural Analysis, and Expression in Escherichia coli of a Chitinase Gene from Enterobacter agglomerans<br>Leonid S. Chernin, Leonardo de la Fuente, Vladimir Sobolev, Shoshan Haran, Constantin E. Vorgias, Amos B. Oppenheim, Ilan Chet<br>Applied and Environmental Microbiology, Mar. 1997, p. 834-839<br><br><br>7.N-Acetylglucosamine: Production and Applications<br>Jeen-Kuan Chen , Chia-Rui Shen&nbsp; and Chao-Lin Liu<br>Marine Drugs, Received: 22 March 2010; in revised form: 19 April 2010 / Accepted: 23 April 2010 / Published: 15 September 2010<br><br><br>8.Optimization of glucose feeding approaches for enhanced<br>glucosamine and N-acetylglucosamine production<br>by an engineered Escherichia coli<br>Xin Chen • Long Liu • Jianghua Li •Jie Liu • Guocheng Du • Jian Chen<br>Received: 19 August 2011 / Accepted: 30 September 2011 / Published online: 19 October 2011 Society for Industrial Microbiology 2011<br><br><br>9.Purification and Antifungal Activity of Recombinant Chitinase from Escherichia coli Carrying the Family 19 Chitinase Gene of Streptomyces sp. J-13-3<br>Yousuke Yamashita and Katsurichiro Okazaki<br>Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan<br>Biosci. Biotechnol. Biochem., 68 (10), 2193-2196, 2004<br><br><br>10.Role of Chitinases in Human Stomach for Chitin Digestion: AMCase in the Gastric Digestion of Chitin and Chit in Gastric Pathologies<br>Maurizio G. Paoletti, Lorenzo Norberto, Elisa Cozzarini and Salvatore Musumeci<br>Binomium Chitin-Chitinase: Recent Issues<br><br><br>11.The Metabolism of Exogenous N-Acetyl-D-glucosamine<br>Biochem. J. (1961) 80, 5726-O[35S]-Sulphate in the Normal Rat<br>BY A. G. LLOYD<br>Department of Biochemi8try, Univer8ity College, Newport Road, Cardiff (Received 6 February 1961)<br><br><br>Find a Vitamin or Supplement N - ACETYL GLUCOSAMINE<br><u><a href="http://www.webmd.com/vitamins-supplements/ingredientmono-619-N-ACETYL+GLUCOSAMINE.aspx?activeIngredientId=619&activeIngredientName=N-ACETYL+GLUCOSAMINE " target="http://www.webmd.com/vitamins-supplements/ingredientmono-619-N-ACETYL+GLUCOSAMINE.aspx?activeIngredientId=619&activeIngredientName=N-ACETYL+GLUCOSAMINE ">&lt;http://www.webmd.com/vitamins-supplements/ingredientmono-619-N-ACETYL+GLUCOSAMINE.aspx?activeIngredientId=619&amp;activeIngredientName=N-ACETYL+GLUCOSAMINE&gt;</u><br></a><br><br>Glucosamine<br><u><a href="http://health.yahoo.net/natstandardcontent/glucosamine" target="http://health.yahoo.net/natstandardcontent/glucosamine">&lt;http://health.yahoo.net/natstandardcontent/glucosamine&gt;</u> </a><br><br><br>Glucosamine Clinical Trial Summaries<br><u><a href="http://www.ultimateglucosamine.com/consumers/clinicaltrials.shtml" target="http://www.ultimateglucosamine.com/consumers/clinicaltrials.shtml">&lt;http://www.ultimateglucosamine.com/consumers/clinicaltrials.shtml&gt;</u><br></a><br><br>Heres How N Acetylglucosamine May Help Crohns (by Brad Chase)<br><u><a href="http://www.progressivehealth.com/learn-how-n-acetylglucosamine-can-help-crohns.htm" target="http://www.progressivehealth.com/learn-how-n-acetylglucosamine-can-help-crohns.htm">&lt;http://www.progressivehealth.com/learn-how-n-acetylglucosamine-can-help-crohns.htm&gt;</u><br></a><br><br>Insulin Resistance and Glucosamine Supplementation<br><u><a href="http://www.ultimateglucosamine.com/consumers/insulinresistance.shtml" target="http://www.ultimateglucosamine.com/consumers/insulinresistance.shtml">&lt;http://www.ultimateglucosamine.com/consumers/insulinresistance.shtml&gt;</u><br></a><br><br>N-Acetyl-D-glucosamine &amp; Glucosamine AbsorptionDistribution And Metabolism <u><a href="http://www.ultimateglucosamine.com/consumers/metabolism.shtml" target="http://www.ultimateglucosamine.com/consumers/metabolism.shtml">&lt;http://www.ultimateglucosamine.com/consumers/metabolism.shtml&gt;</u><br></a><br><br>N-acetylglucosaminidase, alpha<br><u><a href="http://www.ebi.ac.uk/s4/summary/molecular?term=n-acetyl-glucosaminidase&classification=9606&tid=gSynAT5G13690" target="http://www.ebi.ac.uk/s4/summary/molecular?term=n-acetyl-glucosaminidase&classification=9606&tid=gSynAT5G13690">&lt;http://www.ebi.ac.uk/s4/summary/molecular?term=n-acetyl-glucosaminidase&amp;classification=9606&amp;tid=gSynAT5G13690&gt;</u><br></a><br><br>NAGLU »&nbsp; Alpha-N-acetylglucosaminidase<br><u><a href="http://www.nextprot.org/db/entry/NX_P54802/expression" target="http://www.nextprot.org/db/entry/NX_P54802/expression">&lt;http://www.nextprot.org/db/entry/NX_P54802/expression&gt;</u><br></a><br><br><br><br><br></b></font></div></div></body></html><!--
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<a href="http://www.thermoscientificbio.com/uploadedFiles/Resources/k1081-product-information.pdf" target="http://www.thermoscientificbio.com/uploadedFiles/Resources/k1081-product-information.pdf">http://www.thermoscientificbio.com/uploadedFiles/Resources/k1081-product-information.pdf</a><br>
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Latest revision as of 01:09, 21 June 2014


Sources




Sources


1.A Gel Diffusion Assay for Visualization and Quantification of Chitinase Activity
Xiaohong Zou, Hiroyuki Nonogaki, and Gregory E. Welbaum
Molecular Biotechnology, Volume 22, 2002


2.A pilot study of N-acetyl glucosamine, a nutritional substrate for glycosaminoglycan synthesis, in paediatric chronic inflammatory bowel disease
S. Salvatore, R. Heuschkel, S. Tomlin, S. E. Davies, S. Edwards, J. A. Walker-Smith, I. French and S. H. Murch
Alimentary Pharmacology & Therapeutics Alimentary Pharmacology & Therapeutics Volume 14, Issue 12, pages 1567-1579, December 2000 (Article first published online: 2 JAN 2002)


3.Cloning and Expression of a Chitinase Gene from Sanguibacter sp. C4
Tao Yong, Jin Hong, Long Zhang-Fu, Zhang Li, Ding Xiu-Qiong, Tao Ke, Liu Shi-Gui
Acta Genetica Sinica, November 2006


4.Expression and efficient secretion of a functional chitinase from Chromobacterium violaceum in Escherichia coli
Marina Duarte Pinto Lobo, Fredy Davi Albuquerque Silva, Patrícia Gadelha de Castro Landim, Paloma Ribeiro da Cruz, Thaís Lima de Brito, Suelen Carneiro de Medeiros, José Tadeu Abreu Oliveira, Ilka Maria Vasconcelos, Humberto D’Muniz Pereira and Thalles Barbosa Grangeiro
Lobo et al. BMC Biotechnology 2013


5. Metabolic engineering of Escherichia coli for industrial production of glucosamine and N-acetylglucosamine
Ming-De Deng,David K. Severson,Alan D. Grund,Sarah L. Wassink, Richard P. Burlingame,Alan Berry,Jeffrey A. Running,Candice A. Kunesh, Linsheng Song,Thomas A. Jerrell,Reinhardt A. Rosson
Bio-Technical Resources, 1035 South 7th Street, Manitowoc, WI 54220, USA
Received 17 September 2004; accepted 8 February 2005
Available online 23 March 2005


6.Molecular Cloning, Structural Analysis, and Expression in Escherichia coli of a Chitinase Gene from Enterobacter agglomerans
Leonid S. Chernin, Leonardo de la Fuente, Vladimir Sobolev, Shoshan Haran, Constantin E. Vorgias, Amos B. Oppenheim, Ilan Chet
Applied and Environmental Microbiology, Mar. 1997, p. 834-839


7.N-Acetylglucosamine: Production and Applications
Jeen-Kuan Chen , Chia-Rui Shen  and Chao-Lin Liu
Marine Drugs, Received: 22 March 2010; in revised form: 19 April 2010 / Accepted: 23 April 2010 / Published: 15 September 2010


8.Optimization of glucose feeding approaches for enhanced
glucosamine and N-acetylglucosamine production
by an engineered Escherichia coli
Xin Chen • Long Liu • Jianghua Li •Jie Liu • Guocheng Du • Jian Chen
Received: 19 August 2011 / Accepted: 30 September 2011 / Published online: 19 October 2011 Society for Industrial Microbiology 2011


9.Purification and Antifungal Activity of Recombinant Chitinase from Escherichia coli Carrying the Family 19 Chitinase Gene of Streptomyces sp. J-13-3
Yousuke Yamashita and Katsurichiro Okazaki
Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan
Biosci. Biotechnol. Biochem., 68 (10), 2193-2196, 2004


10.Role of Chitinases in Human Stomach for Chitin Digestion: AMCase in the Gastric Digestion of Chitin and Chit in Gastric Pathologies
Maurizio G. Paoletti, Lorenzo Norberto, Elisa Cozzarini and Salvatore Musumeci
Binomium Chitin-Chitinase: Recent Issues


11.The Metabolism of Exogenous N-Acetyl-D-glucosamine
Biochem. J. (1961) 80, 5726-O[35S]-Sulphate in the Normal Rat
BY A. G. LLOYD
Department of Biochemi8try, Univer8ity College, Newport Road, Cardiff (Received 6 February 1961)


Find a Vitamin or Supplement N - ACETYL GLUCOSAMINE
<http://www.webmd.com/vitamins-supplements/ingredientmono-619-N-ACETYL+GLUCOSAMINE.aspx?activeIngredientId=619&activeIngredientName=N-ACETYL+GLUCOSAMINE>


Glucosamine
<http://health.yahoo.net/natstandardcontent/glucosamine>


Glucosamine Clinical Trial Summaries
<http://www.ultimateglucosamine.com/consumers/clinicaltrials.shtml>


Heres How N Acetylglucosamine May Help Crohns (by Brad Chase)
<http://www.progressivehealth.com/learn-how-n-acetylglucosamine-can-help-crohns.htm>


Insulin Resistance and Glucosamine Supplementation
<http://www.ultimateglucosamine.com/consumers/insulinresistance.shtml>


N-Acetyl-D-glucosamine & Glucosamine AbsorptionDistribution And Metabolism <http://www.ultimateglucosamine.com/consumers/metabolism.shtml>


N-acetylglucosaminidase, alpha
<http://www.ebi.ac.uk/s4/summary/molecular?term=n-acetyl-glucosaminidase&classification=9606&tid=gSynAT5G13690>


NAGLU »  Alpha-N-acetylglucosaminidase
<http://www.nextprot.org/db/entry/NX_P54802/expression>

Articles

Quorum sensing and Chromobacterium violaceum: exploitation of violacein production
and inhibition for the detection of N-acylhomoserine lactones
Kay H. McClean, Michael K. Winson, Leigh Fish, Adrian Taylor, Siri Ram Chhabra, Miguel
Camara, Mavis Daykin, John H. Lamb, Simon Swift, Barrie W. Bycroft, Gordon S. A. B. Stewart
and Paul Williams
Microbiology, Issue 143, Pages 3703–3711 , 1997
http://www.redmicrobios.com.ar/pdf/22-CV026-1.pdf

Cloning, Sequencing, and Expression of the Chitinase Gene chiA74 from Bacillus
thuringiensis
J. Eleazar Barboza-Corona, Elizabeth Nieto-Mazzocco, [...], and Jorge E. Ibarra
Applied and Enviromental Microbiology, Issue 69(2), Pages 1023-1029, February 2003
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC143672/

Cloning of chitinase gene from Bacillus thuringiensis
T R Usharani* and T K S Gowda
Indian Journal of Biotechnology
Vol 10, July 2011, pp 264-269
http://nopr.niscair.res.in/bitstream/123456789/12108/1/IJBT%2010%283%29%20264-269.pdf

Other sources

Plasmid data sheet
http://www.expressys.com/pza31luc.pdf

A biokémia és molekuláris biológia alapjai (könyv)
http://elte.prompt.hu/sites/default/files/tananyagok/biokemia_alapjai/BIO7_Biokemia_alapjai.pdf

Plasmid map – pZA31-Luc vector
https://benchling.com/bhussey/vectors/MgM-pza31-luc/

Bacillus thuringiensis chitinase (chi) gene, complete cds, GenBank: AF424979.1
http://www.ncbi.nlm.nih.gov/nuccore/16326732

Chitinase [Bacillus thuringiensis], GenBank: AAL17867.1
http://www.ncbi.nlm.nih.gov/protein/16326733

Bacillus thuringiensis chitinase (chi74) gene, complete cds, GenBank: EF197878.1
http://www.ncbi.nlm.nih.gov/nuccore/EF197878.1

Chitinase [Bacillus thuringiensis serovar kurstaki], GenBank: ABQ65137.2
http://www.ncbi.nlm.nih.gov/protein/149087537?report=genbank&log$=prottop&blast_rank=9&RID=TTBWRNZD014

Chitinase production by Bacillus thuringiensis and Bacillus licheniformis: their potential in antifungal biocontrol.
http://www.ncbi.nlm.nih.gov/pubmed/22367944

Bacterial Transformation
http://www.addgene.org/plasmid_protocols/bacterial_transformation/

DNA Ligation
http://www.addgene.org/plasmid_protocols/DNA_ligation/

Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning)
http://www.addgene.org/plasmid_protocols/subcloning/

Restriction Digest of Plasmid DNA
http://www.addgene.org/plasmid_protocols/restriction_digest/

Popular Plasmids
https://www.addgene.org/popular-plasmids/

BLAST
http://blast.ncbi.nlm.nih.gov/Blast.cgi#149087537

Sequence: AF424979.1 : Bacillus thuringiensis chitinase (chi) gene, complete cds.
http://www.ebi.ac.uk/ena/data/view/AF424979

Coding: AAL17867.1 : Bacillus thuringiensis chitinase
http://www.ebi.ac.uk/ena/data/view/AAL17867

NCAIM 0025 Nutrient agar
http://web.uni-corvinus.hu:8089/NCAIM/ncaim/XmediumDetail.jsp?MID=23

DESIGN PCR PRIMERS
http://molbiol-tools.ca/PCR.htm

Hungarian legislation
http://gmo.kormany.hu/hungarian-legislation

Assembly:Standard assembly
http://parts.igem.org/Assembly:Standard_assembly

An Introduction to BioBricks
http://parts.igem.org/Help:An_Introduction_to_BioBricks

BioBrick Prefix and Suffix
http://parts.igem.org/Help:BioBrick_Prefix_and_Suffix

Standardization
http://parts.igem.org/Help:Standardization

Assembly:RBS-CDS issues
http://parts.igem.org/Assembly:RBS-CDS_issues

Part:pSB1C3:Design
http://parts.igem.org/Part:pSB1C3:Design

Prefix and Suffix
http://parts.igem.org/Help:Prefix-Suffix

Part:BBa_J04450
http://parts.igem.org/Part:BBa_J04450

Laboratory biosafety manual - ISBN 92 4 154650 6
http://www.who.int/csr/resources/publications/biosafety/Biosafety7.pdf

Safety – iGEM
https://2014hs.igem.org/Safety

iGEM 2013 Basic Safety Form
https://static.igem.org/mediawiki/2013/8/86/IGEM_2013_Basic_Safety_Form_BLANK.pdf

Antibiotic usage
https://www.k-state.edu/hermanlab/protocols/AntibioticUsage.html

Bacterial Genomic DNA
http://www.dsmz.de/services/services-microorganisms/bacterial-genomic-dna.html

Product information - ThermoScientific DreamTaq Green PCR Master Mix (2X) # K1081 200
http://www.thermoscientificbio.com/uploadedFiles/Resources/k1081-product-information.pdf