1.A Gel Diffusion Assay for Visualization and Quantification of Chitinase Activity
Xiaohong Zou, Hiroyuki Nonogaki, and Gregory E. Welbaum
Molecular Biotechnology, Volume 22, 2002

2.A pilot study of N-acetyl glucosamine, a nutritional substrate for glycosaminoglycan synthesis, in paediatric chronic inflammatory bowel disease
S. Salvatore, R. Heuschkel, S. Tomlin, S. E. Davies, S. Edwards, J. A. Walker-Smith, I. French and S. H. Murch
Alimentary Pharmacology & Therapeutics Alimentary Pharmacology & Therapeutics Volume 14, Issue 12, pages 1567-1579, December 2000 (Article first published online: 2 JAN 2002)

3.Cloning and Expression of a Chitinase Gene from Sanguibacter sp. C4
Tao Yong, Jin Hong, Long Zhang-Fu, Zhang Li, Ding Xiu-Qiong, Tao Ke, Liu Shi-Gui
Acta Genetica Sinica, November 2006

4.Expression and efficient secretion of a functional chitinase from Chromobacterium violaceum in Escherichia coli
Marina Duarte Pinto Lobo, Fredy Davi Albuquerque Silva, Patrícia Gadelha de Castro Landim, Paloma Ribeiro da Cruz, Thaís Lima de Brito, Suelen Carneiro de Medeiros, José Tadeu Abreu Oliveira, Ilka Maria Vasconcelos, Humberto D’Muniz Pereira and Thalles Barbosa Grangeiro
Lobo et al. BMC Biotechnology 2013

5. Metabolic engineering of Escherichia coli for industrial production of glucosamine and N-acetylglucosamine
Ming-De Deng,David K. Severson,Alan D. Grund,Sarah L. Wassink, Richard P. Burlingame,Alan Berry,Jeffrey A. Running,Candice A. Kunesh, Linsheng Song,Thomas A. Jerrell,Reinhardt A. Rosson
Bio-Technical Resources, 1035 South 7th Street, Manitowoc, WI 54220, USA
Received 17 September 2004; accepted 8 February 2005
Available online 23 March 2005

6.Molecular Cloning, Structural Analysis, and Expression in Escherichia coli of a Chitinase Gene from Enterobacter agglomerans
Leonid S. Chernin, Leonardo de la Fuente, Vladimir Sobolev, Shoshan Haran, Constantin E. Vorgias, Amos B. Oppenheim, Ilan Chet
Applied and Environmental Microbiology, Mar. 1997, p. 834-839

7.N-Acetylglucosamine: Production and Applications
Jeen-Kuan Chen , Chia-Rui Shen  and Chao-Lin Liu
Marine Drugs, Received: 22 March 2010; in revised form: 19 April 2010 / Accepted: 23 April 2010 / Published: 15 September 2010

8.Optimization of glucose feeding approaches for enhanced
glucosamine and N-acetylglucosamine production
by an engineered Escherichia coli
Xin Chen • Long Liu • Jianghua Li •Jie Liu • Guocheng Du • Jian Chen
Received: 19 August 2011 / Accepted: 30 September 2011 / Published online: 19 October 2011 Society for Industrial Microbiology 2011

9.Purification and Antifungal Activity of Recombinant Chitinase from Escherichia coli Carrying the Family 19 Chitinase Gene of Streptomyces sp. J-13-3
Yousuke Yamashita and Katsurichiro Okazaki
Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan
Biosci. Biotechnol. Biochem., 68 (10), 2193-2196, 2004

10.Role of Chitinases in Human Stomach for Chitin Digestion: AMCase in the Gastric Digestion of Chitin and Chit in Gastric Pathologies
Maurizio G. Paoletti, Lorenzo Norberto, Elisa Cozzarini and Salvatore Musumeci
Binomium Chitin-Chitinase: Recent Issues

11.The Metabolism of Exogenous N-Acetyl-D-glucosamine
Biochem. J. (1961) 80, 5726-O[35S]-Sulphate in the Normal Rat
Department of Biochemi8try, Univer8ity College, Newport Road, Cardiff (Received 6 February 1961)

Find a Vitamin or Supplement N - ACETYL GLUCOSAMINE


Glucosamine Clinical Trial Summaries

Heres How N Acetylglucosamine May Help Crohns (by Brad Chase)

Insulin Resistance and Glucosamine Supplementation

N-Acetyl-D-glucosamine & Glucosamine AbsorptionDistribution And Metabolism <>

N-acetylglucosaminidase, alpha

NAGLU »  Alpha-N-acetylglucosaminidase


Quorum sensing and Chromobacterium violaceum: exploitation of violacein production
and inhibition for the detection of N-acylhomoserine lactones
Kay H. McClean, Michael K. Winson, Leigh Fish, Adrian Taylor, Siri Ram Chhabra, Miguel
Camara, Mavis Daykin, John H. Lamb, Simon Swift, Barrie W. Bycroft, Gordon S. A. B. Stewart
and Paul Williams
Microbiology, Issue 143, Pages 3703–3711 , 1997

Cloning, Sequencing, and Expression of the Chitinase Gene chiA74 from Bacillus
J. Eleazar Barboza-Corona, Elizabeth Nieto-Mazzocco, [...], and Jorge E. Ibarra
Applied and Enviromental Microbiology, Issue 69(2), Pages 1023-1029, February 2003

Cloning of chitinase gene from Bacillus thuringiensis
T R Usharani* and T K S Gowda
Indian Journal of Biotechnology
Vol 10, July 2011, pp 264-269

Other sources

Plasmid data sheet

A biokémia és molekuláris biológia alapjai (könyv)

Plasmid map – pZA31-Luc vector

Bacillus thuringiensis chitinase (chi) gene, complete cds, GenBank: AF424979.1

Chitinase [Bacillus thuringiensis], GenBank: AAL17867.1

Bacillus thuringiensis chitinase (chi74) gene, complete cds, GenBank: EF197878.1

Chitinase [Bacillus thuringiensis serovar kurstaki], GenBank: ABQ65137.2$=prottop&blast_rank=9&RID=TTBWRNZD014

Chitinase production by Bacillus thuringiensis and Bacillus licheniformis: their potential in antifungal biocontrol.

Bacterial Transformation

DNA Ligation

Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning)

Restriction Digest of Plasmid DNA

Popular Plasmids


Sequence: AF424979.1 : Bacillus thuringiensis chitinase (chi) gene, complete cds.

Coding: AAL17867.1 : Bacillus thuringiensis chitinase

NCAIM 0025 Nutrient agar


Hungarian legislation

Assembly:Standard assembly

An Introduction to BioBricks

BioBrick Prefix and Suffix


Assembly:RBS-CDS issues


Prefix and Suffix


Laboratory biosafety manual - ISBN 92 4 154650 6

Safety – iGEM

iGEM 2013 Basic Safety Form

Antibiotic usage

Bacterial Genomic DNA

Product information - ThermoScientific DreamTaq Green PCR Master Mix (2X) # K1081 200