Team:HTHS Trussville AL/Team Log

From 2014hs.igem.org

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(Created page with "1.)Determine if the PhO sensor is the one that we want to use. 2.)Find the yeast plasmid vector to put the sequence into 3.)Determine the restriction enzymes needed to cut the pl...")
(→September 23, 2013)
 
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1.)Determine if the PhO sensor is the one that we want to use.
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== August 26, 2013==
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2.)Find the yeast plasmid vector to put the sequence into
+
August 26, 2013:
-
3.)Determine the restriction enzymes needed to cut the plasmid and put the sequence in.
+
 
-
4.)Get the red fluorescent protein (RFP) DNA sequence
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1)Determine if the Pho Sensor is the one the group wants to use
-
5.)Find and determine if the invertor DNA sequence will work
+
 
-
6.)Gibson Assembly
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2)Find the yeast plasmid vector to put the sequence into
-
7.)Put plasmid through PCR
+
 
-
8.)Test with electrophoresis
+
3)Determine the restriction enzyme needed to cut the plasmid (and put the sequence in)
-
9.)Cut sequences out of gels if working
+
 
-
10.)Inject plasmid into yeast
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4)Get RFP (Red Fluorescent Protein) DNA sequence
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11.)Grow yeast
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12.)Test yeast
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5)Find and determine if the inverter DNA sequence will work
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13.)Develop standardization test for red color
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14.)Water quality testing
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6)Gibson Assembly
 +
 
 +
7)Put plasmid through PCR
 +
 
 +
8)Test with electrophoresis  
 +
 
 +
9)Cut sequences out of gels if working  
 +
 
 +
10)Inject plasmid into yeast
 +
 
 +
11)Grow Yeast
 +
 
 +
12)Test Yeast
 +
 
 +
13)Develop standardization test for red color
 +
 
 +
14)Water quality test
 +
 
 +
== September 17, 2013 ==
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To Dos:
 +
 
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1.)Download new software (Tinkercell and GENtle)
 +
 
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2.)Re-do Gantt chart
 +
 
 +
3.)Redraw plasmid
 +
 
 +
4.)Start a list of potential questions and problems
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 +
5.)Finish scholarly article reviews
 +
 
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6.)Put DNA sequences into software
 +
 
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-Find Pho 5 sequence and promoter
 +
 
 +
-Find RFP sequence
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 +
-Find plasmid
 +
 
 +
--Verify origin of replication
 +
--Verify antibiotic resistance
 +
--Antibiotic vs. antifungal
 +
 
 +
7.)Discuss restriction enzymes
 +
*See Figure 1: First Plasmid Model
 +
 
 +
== September 23, 2013 ==
 +
 
 +
First skype meeting with Dr. Zahorchak
 +
 
 +
Remove inverter from the plasmid

Latest revision as of 03:01, 21 June 2014

August 26, 2013

August 26, 2013:

1)Determine if the Pho Sensor is the one the group wants to use

2)Find the yeast plasmid vector to put the sequence into

3)Determine the restriction enzyme needed to cut the plasmid (and put the sequence in)

4)Get RFP (Red Fluorescent Protein) DNA sequence

5)Find and determine if the inverter DNA sequence will work

6)Gibson Assembly

7)Put plasmid through PCR

8)Test with electrophoresis

9)Cut sequences out of gels if working

10)Inject plasmid into yeast

11)Grow Yeast

12)Test Yeast

13)Develop standardization test for red color

14)Water quality test

September 17, 2013

To Dos:

1.)Download new software (Tinkercell and GENtle)

2.)Re-do Gantt chart

3.)Redraw plasmid

4.)Start a list of potential questions and problems

5.)Finish scholarly article reviews

6.)Put DNA sequences into software

-Find Pho 5 sequence and promoter

-Find RFP sequence

-Find plasmid

--Verify origin of replication --Verify antibiotic resistance --Antibiotic vs. antifungal

7.)Discuss restriction enzymes

  • See Figure 1: First Plasmid Model

September 23, 2013

First skype meeting with Dr. Zahorchak

Remove inverter from the plasmid

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