"
Team:HTHS Trussville AL/Team Log
From 2014hs.igem.org
Chloe.wilks (Talk | contribs) (→August , 2013) |
Chloe.wilks (Talk | contribs) (→September 23, 2013) |
||
| (2 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
== August 26, 2013== | == August 26, 2013== | ||
August 26, 2013: | August 26, 2013: | ||
| + | |||
1)Determine if the Pho Sensor is the one the group wants to use | 1)Determine if the Pho Sensor is the one the group wants to use | ||
| + | |||
2)Find the yeast plasmid vector to put the sequence into | 2)Find the yeast plasmid vector to put the sequence into | ||
| + | |||
3)Determine the restriction enzyme needed to cut the plasmid (and put the sequence in) | 3)Determine the restriction enzyme needed to cut the plasmid (and put the sequence in) | ||
| + | |||
4)Get RFP (Red Fluorescent Protein) DNA sequence | 4)Get RFP (Red Fluorescent Protein) DNA sequence | ||
| + | |||
5)Find and determine if the inverter DNA sequence will work | 5)Find and determine if the inverter DNA sequence will work | ||
| + | |||
6)Gibson Assembly | 6)Gibson Assembly | ||
| + | |||
7)Put plasmid through PCR | 7)Put plasmid through PCR | ||
| + | |||
8)Test with electrophoresis | 8)Test with electrophoresis | ||
| + | |||
9)Cut sequences out of gels if working | 9)Cut sequences out of gels if working | ||
| + | |||
10)Inject plasmid into yeast | 10)Inject plasmid into yeast | ||
| + | |||
11)Grow Yeast | 11)Grow Yeast | ||
| + | |||
12)Test Yeast | 12)Test Yeast | ||
| + | |||
13)Develop standardization test for red color | 13)Develop standardization test for red color | ||
| + | |||
14)Water quality test | 14)Water quality test | ||
| Line 43: | Line 57: | ||
7.)Discuss restriction enzymes | 7.)Discuss restriction enzymes | ||
| + | *See Figure 1: First Plasmid Model | ||
| + | |||
| + | == September 23, 2013 == | ||
| - | + | First skype meeting with Dr. Zahorchak | |
| - | + | Remove inverter from the plasmid | |
Latest revision as of 03:01, 21 June 2014
August 26, 2013
August 26, 2013:
1)Determine if the Pho Sensor is the one the group wants to use
2)Find the yeast plasmid vector to put the sequence into
3)Determine the restriction enzyme needed to cut the plasmid (and put the sequence in)
4)Get RFP (Red Fluorescent Protein) DNA sequence
5)Find and determine if the inverter DNA sequence will work
6)Gibson Assembly
7)Put plasmid through PCR
8)Test with electrophoresis
9)Cut sequences out of gels if working
10)Inject plasmid into yeast
11)Grow Yeast
12)Test Yeast
13)Develop standardization test for red color
14)Water quality test
September 17, 2013
To Dos:
1.)Download new software (Tinkercell and GENtle)
2.)Re-do Gantt chart
3.)Redraw plasmid
4.)Start a list of potential questions and problems
5.)Finish scholarly article reviews
6.)Put DNA sequences into software
-Find Pho 5 sequence and promoter
-Find RFP sequence
-Find plasmid
--Verify origin of replication --Verify antibiotic resistance --Antibiotic vs. antifungal
7.)Discuss restriction enzymes
- See Figure 1: First Plasmid Model
September 23, 2013
First skype meeting with Dr. Zahorchak
Remove inverter from the plasmid
