2014hs.igem.org/team:Charlottesville RS/Notebook/041514

From 2014hs.igem.org

April 15th, 2014

Nick Keen, Anders Beaurline

Took cells off of plate that were incubating overnight. Suspended them in calcium chloride and put them on ice for 15 minutes. Switched from Carolina procedure to our own starting at step 6 of the “producing competent cells” sheet, then centrifuged and resuspended cells in cold calcium chloride.

Anders Beaurline

Mixed competent cells with DNA of concentrations (in pg/uL): 50, 25, 10, 5, .5. Incubated on ice for 30 minutes, then heat shocked, then put back on ice.

Becky Wilbur

Plated transformed (we hope) cells as in step number 7 on page 29 in the iGem procedure notebook and put in 37 degrees (Celsius) incubator.