Lab Protocol Successes - Team OLeSsence
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*Need to build gel illuminator next year |
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<td><ul> | <td><ul> | ||
- | <li>- Mastered use of pressure-steam autoclave for all sterilization/decontamination</li> | + | <li align="left">- Mastered use of pressure-steam autoclave for all sterilization/decontamination</li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><ul> | <td><ul> | ||
- | <li>- Routinely preparing our own LB-agar plates (with and without antibiotic) and LB broth for cell culture.</li> | + | <li align="left">- Routinely preparing our own LB-agar plates (with and without antibiotic) and LB broth for cell culture.</li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
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<td><ul> | <td><ul> | ||
- | <li>- Successful plating techniques for E. coli – routinely growing single colonies free from contamination using aseptic technique.</li> | + | <li align="left">- Successful plating techniques for E. coli – routinely growing single colonies free from contamination using aseptic technique.</li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
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<td><ul> | <td><ul> | ||
- | <li>- Successfully growing cell cultures from individual colonies in DIY shaker/incubator</li> | + | <li align="left">- Successfully growing cell cultures from individual colonies in DIY shaker/incubator</li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
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<td><ul> | <td><ul> | ||
- | <li>- Successfully grew E. coli cells (provided from iGEM) containing our parts (K098995 and J45119), including cell cultures.</li> | + | <li align="left">- Successfully grew E. coli cells (provided from iGEM) containing our parts (K098995 and J45119), including cell cultures.</li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><ul> | <td><ul> | ||
- | <li>- Mastered use of DIY Dremelfuge (confirming RPM with photogate) to pellet DNA</li> | + | <li align="left">- Mastered use of DIY Dremelfuge (confirming RPM with photogate) to pellet DNA</li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><ul> | <td><ul> | ||
- | <li>- Successfully mini-prepped both our parts to extract plasmid DNA</li> | + | <li align="left">- Successfully mini-prepped both our parts to extract plasmid DNA</li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
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<td><ul> | <td><ul> | ||
- | <li> - Successfully used restriction digest protocols to cut our plasmids of interest, confirming presence of DNA strands of expected size via gel electrophoresis</li> | + | <li align="left"> - Successfully used restriction digest protocols to cut our plasmids of interest, confirming presence of DNA strands of expected size via gel electrophoresis</li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
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<table width="100%" border="0" cellspacing="1" cellpadding="10"> | <table width="100%" border="0" cellspacing="1" cellpadding="10"> | ||
<tr> | <tr> | ||
- | <td>*Need to build gel illuminator next year</td> | + | <td align="left">*Need to build gel illuminator next year</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><ul> | <td><ul> | ||
- | <li>- Successfully transformed RFP plasmid into competent cells (prepped by our mentors) to grow RFP colonies.</li> | + | <li align="left">- Successfully transformed RFP plasmid into competent cells (prepped by our mentors) to grow RFP colonies.</li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
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<table width="100%" border="0" cellspacing="1" cellpadding="10"> | <table width="100%" border="0" cellspacing="1" cellpadding="10"> | ||
<tr> | <tr> | ||
- | <td>=============================================================================</td> | + | <td>=======================================================================================================</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><blockquote> | <td><blockquote> | ||
- | <p>- Successful completion of the 3A Protocols, including:<br> | + | <p align="left">- Successful completion of the 3A Protocols, including:<br> |
</p> | </p> | ||
</blockquote></td> | </blockquote></td> | ||
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<td><blockquote> | <td><blockquote> | ||
<blockquote> | <blockquote> | ||
- | <p>- Restriction digest of plasmids<br> | + | <p align="left">- Restriction digest of plasmids<br> |
- Gel electrophoresis of plasmid digest (NB: No evidence of DNA on gel)<br> | - Gel electrophoresis of plasmid digest (NB: No evidence of DNA on gel)<br> | ||
- Ligation protocol </p> | - Ligation protocol </p> |
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*Need to build gel illuminator next year |
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======================================================================================================= |
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