Team:FHS Frederick MD/Notebook
From 2014hs.igem.org
(Difference between revisions)
(→Lab Notebook) |
(Added footer) |
||
Line 24: | Line 24: | ||
* [http://arsbiotechnica.org/w/Record:002-029 09 Jun 2014]: Submitted samples for sequencing | * [http://arsbiotechnica.org/w/Record:002-029 09 Jun 2014]: Submitted samples for sequencing | ||
* [http://arsbiotechnica.org/w/Record:002-030 11 Jun 2014]: Resubmitted DNA for sequencing | * [http://arsbiotechnica.org/w/Record:002-030 11 Jun 2014]: Resubmitted DNA for sequencing | ||
+ | {{:Team:FHS_Frederick_MD/Footer}} |
Revision as of 01:29, 21 June 2014
Lab Notebook
- 20 Feb 2014: Received iGEM supplies
- 03 Apr 2014: Digested NirB and LOV inserts
- 10 Apr 2014: Ligated NirB and LOV genes into pSB1C3
- 14 Apr 2014: Transformed E. coli with LOV and NirB constructs
- 21 Apr 2014: Ligated NirB and LOV genes into pSB1C3
- 28 Apr 2014: Transformed E. coli with LOV and NirB constructs
- 01 May 2014: Prepared TSS buffer
- 05 May 2014: Ligatied the RFP plasmid
- 08 May 2014: Subcultured E. coli NEB10 Beta
- 08 May 2014: Tested transformation efficiency of competent cells
- 12 May 2014: Digested NirB, LOV, pSB1C3 DNA fragments with EcoRI and PstI
- 15 May 2014: Ligated NirB and LOV into pSB1C3
- 20 May 2014: Transformed E. coli with LOV and NirB constructs
- 29 May 2014: Cultured individual NirB and LOV colonies
- 02 Jun 2014: Purified NirB and LOV plasmids
- 03 Jun 2014: Visualized NirB and LOV plasmids on a gel
- 04 Jun 2014: Recultured NirB and LOV transformants
- 05 Jun 2014: Extracted plasmid DNA from bacterial transformants
- 06 Jun 2014: Observed plasmid DNA on a gel
- 09 Jun 2014: Submitted samples for sequencing
- 11 Jun 2014: Resubmitted DNA for sequencing