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| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Elegant"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Professional"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Subtle 1"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Subtle 2"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Web 1"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Web 2"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Web 3"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Balloon Text"/>
| |
| - | <w:LsdException Locked="false" Priority="39" Name="Table Grid"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Theme"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" Name="Placeholder Text"/>
| |
| - | <w:LsdException Locked="false" Priority="1" QFormat="true" Name="No Spacing"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 1"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" Name="Revision"/>
| |
| - | <w:LsdException Locked="false" Priority="34" QFormat="true"
| |
| - | Name="List Paragraph"/>
| |
| - | <w:LsdException Locked="false" Priority="29" QFormat="true" Name="Quote"/>
| |
| - | <w:LsdException Locked="false" Priority="30" QFormat="true"
| |
| - | Name="Intense Quote"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="19" QFormat="true"
| |
| - | Name="Subtle Emphasis"/>
| |
| - | <w:LsdException Locked="false" Priority="21" QFormat="true"
| |
| - | Name="Intense Emphasis"/>
| |
| - | <w:LsdException Locked="false" Priority="31" QFormat="true"
| |
| - | Name="Subtle Reference"/>
| |
| - | <w:LsdException Locked="false" Priority="32" QFormat="true"
| |
| - | Name="Intense Reference"/>
| |
| - | <w:LsdException Locked="false" Priority="33" QFormat="true" Name="Book Title"/>
| |
| - | <w:LsdException Locked="false" Priority="37" SemiHidden="true"
| |
| - | UnhideWhenUsed="true" Name="Bibliography"/>
| |
| - | <w:LsdException Locked="false" Priority="39" SemiHidden="true"
| |
| - | UnhideWhenUsed="true" QFormat="true" Name="TOC Heading"/>
| |
| - | <w:LsdException Locked="false" Priority="41" Name="Plain Table 1"/>
| |
| - | <w:LsdException Locked="false" Priority="42" Name="Plain Table 2"/>
| |
| - | <w:LsdException Locked="false" Priority="43" Name="Plain Table 3"/>
| |
| - | <w:LsdException Locked="false" Priority="44" Name="Plain Table 4"/>
| |
| - | <w:LsdException Locked="false" Priority="45" Name="Plain Table 5"/>
| |
| - | <w:LsdException Locked="false" Priority="40" Name="Grid Table Light"/>
| |
| - | <w:LsdException Locked="false" Priority="46" Name="Grid Table 1 Light"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark"/>
| |
| - | <w:LsdException Locked="false" Priority="51" Name="Grid Table 6 Colorful"/>
| |
| - | <w:LsdException Locked="false" Priority="52" Name="Grid Table 7 Colorful"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="46" Name="List Table 1 Light"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark"/>
| |
| - | <w:LsdException Locked="false" Priority="51" Name="List Table 6 Colorful"/>
| |
| - | <w:LsdException Locked="false" Priority="52" Name="List Table 7 Colorful"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 6"/>
| |
| - | </w:LatentStyles>
| |
| - | </xml><![endif]-->
| |
| - | <style>
| |
| - | <!--
| |
| - | /* Font Definitions */
| |
| - | @font-face
| |
| - | {font-family:"Cambria Math";
| |
| - | panose-1:2 4 5 3 5 4 6 3 2 4;
| |
| - | mso-font-charset:1;
| |
| - | mso-generic-font-family:roman;
| |
| - | mso-font-format:other;
| |
| - | mso-font-pitch:variable;
| |
| - | mso-font-signature:0 0 0 0 0 0;}
| |
| - | @font-face
| |
| - | {font-family:Calibri;
| |
| - | panose-1:2 15 5 2 2 2 4 3 2 4;
| |
| - | mso-font-charset:162;
| |
| - | mso-generic-font-family:swiss;
| |
| - | mso-font-pitch:variable;
| |
| - | mso-font-signature:-536870145 1073786111 1 0 415 0;}
| |
| - | @font-face
| |
| - | {font-family:"Trebuchet MS";
| |
| - | panose-1:2 11 6 3 2 2 2 2 2 4;
| |
| - | mso-font-charset:162;
| |
| - | mso-generic-font-family:swiss;
| |
| - | mso-font-pitch:variable;
| |
| - | mso-font-signature:647 3 0 0 159 0;}
| |
| - | /* Style Definitions */
| |
| - | p.MsoNormal, li.MsoNormal, div.MsoNormal
| |
| - | {mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-parent:"";
| |
| - | margin:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | font-size:11.0pt;
| |
| - | font-family:"Arial","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:minor-fareast;
| |
| - | color:black;}
| |
| - | h1
| |
| - | {mso-style-priority:9;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-link:"Başlık 1 Char";
| |
| - | margin-top:10.0pt;
| |
| - | margin-right:0cm;
| |
| - | margin-bottom:0cm;
| |
| - | margin-left:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | page-break-after:avoid;
| |
| - | mso-outline-level:1;
| |
| - | font-size:16.0pt;
| |
| - | font-family:"Trebuchet MS","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:minor-fareast;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | color:black;
| |
| - | font-weight:normal;}
| |
| - | h2
| |
| - | {mso-style-priority:9;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-link:"Başlık 2 Char";
| |
| - | margin-top:10.0pt;
| |
| - | margin-right:0cm;
| |
| - | margin-bottom:0cm;
| |
| - | margin-left:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | page-break-after:avoid;
| |
| - | mso-outline-level:2;
| |
| - | font-size:13.0pt;
| |
| - | font-family:"Trebuchet MS","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:minor-fareast;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | color:black;}
| |
| - | h3
| |
| - | {mso-style-priority:9;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-link:"Başlık 3 Char";
| |
| - | margin-top:8.0pt;
| |
| - | margin-right:0cm;
| |
| - | margin-bottom:0cm;
| |
| - | margin-left:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | page-break-after:avoid;
| |
| - | mso-outline-level:3;
| |
| - | font-size:12.0pt;
| |
| - | font-family:"Trebuchet MS","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:minor-fareast;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | color:#666666;}
| |
| - | h4
| |
| - | {mso-style-priority:9;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-link:"Başlık 4 Char";
| |
| - | margin-top:8.0pt;
| |
| - | margin-right:0cm;
| |
| - | margin-bottom:0cm;
| |
| - | margin-left:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | page-break-after:avoid;
| |
| - | mso-outline-level:4;
| |
| - | font-size:11.0pt;
| |
| - | font-family:"Trebuchet MS","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:minor-fareast;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | color:#666666;
| |
| - | font-weight:normal;
| |
| - | text-decoration:underline;
| |
| - | text-underline:single;}
| |
| - | h5
| |
| - | {mso-style-priority:9;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-link:"Başlık 5 Char";
| |
| - | margin-top:8.0pt;
| |
| - | margin-right:0cm;
| |
| - | margin-bottom:0cm;
| |
| - | margin-left:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | page-break-after:avoid;
| |
| - | mso-outline-level:5;
| |
| - | font-size:11.0pt;
| |
| - | font-family:"Trebuchet MS","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:minor-fareast;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | color:#666666;
| |
| - | font-weight:normal;}
| |
| - | h6
| |
| - | {mso-style-priority:9;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-link:"Başlık 6 Char";
| |
| - | margin-top:8.0pt;
| |
| - | margin-right:0cm;
| |
| - | margin-bottom:0cm;
| |
| - | margin-left:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | page-break-after:avoid;
| |
| - | mso-outline-level:6;
| |
| - | font-size:11.0pt;
| |
| - | font-family:"Trebuchet MS","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:minor-fareast;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | color:#666666;
| |
| - | font-weight:normal;
| |
| - | font-style:italic;}
| |
| - | p.MsoTitle, li.MsoTitle, div.MsoTitle
| |
| - | {mso-style-priority:10;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-link:"Konu Başlığı Char";
| |
| - | margin:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | page-break-after:avoid;
| |
| - | font-size:21.0pt;
| |
| - | font-family:"Trebuchet MS","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:minor-fareast;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | color:black;}
| |
| - | p.MsoSubtitle, li.MsoSubtitle, div.MsoSubtitle
| |
| - | {mso-style-priority:11;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-link:"Altyazı Char";
| |
| - | margin-top:0cm;
| |
| - | margin-right:0cm;
| |
| - | margin-bottom:10.0pt;
| |
| - | margin-left:0cm;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | page-break-after:avoid;
| |
| - | font-size:13.0pt;
| |
| - | font-family:"Trebuchet MS","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:minor-fareast;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | color:#666666;
| |
| - | font-style:italic;}
| |
| - | span.Balk1Char
| |
| - | {mso-style-name:"Başlık 1 Char";
| |
| - | mso-style-priority:9;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-locked:yes;
| |
| - | mso-style-link:"Başlık 1";
| |
| - | mso-ansi-font-size:16.0pt;
| |
| - | mso-bidi-font-size:16.0pt;
| |
| - | font-family:"Calibri Light","sans-serif";
| |
| - | mso-ascii-font-family:"Calibri Light";
| |
| - | mso-ascii-theme-font:major-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:major-fareast;
| |
| - | mso-hansi-font-family:"Calibri Light";
| |
| - | mso-hansi-theme-font:major-latin;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | mso-bidi-theme-font:major-bidi;
| |
| - | color:#2E74B5;
| |
| - | mso-themecolor:accent1;
| |
| - | mso-themeshade:191;}
| |
| - | span.Balk2Char
| |
| - | {mso-style-name:"Başlık 2 Char";
| |
| - | mso-style-noshow:yes;
| |
| - | mso-style-priority:9;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-locked:yes;
| |
| - | mso-style-link:"Başlık 2";
| |
| - | mso-ansi-font-size:13.0pt;
| |
| - | mso-bidi-font-size:13.0pt;
| |
| - | font-family:"Calibri Light","sans-serif";
| |
| - | mso-ascii-font-family:"Calibri Light";
| |
| - | mso-ascii-theme-font:major-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:major-fareast;
| |
| - | mso-hansi-font-family:"Calibri Light";
| |
| - | mso-hansi-theme-font:major-latin;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | mso-bidi-theme-font:major-bidi;
| |
| - | color:#2E74B5;
| |
| - | mso-themecolor:accent1;
| |
| - | mso-themeshade:191;}
| |
| - | span.Balk3Char
| |
| - | {mso-style-name:"Başlık 3 Char";
| |
| - | mso-style-noshow:yes;
| |
| - | mso-style-priority:9;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-locked:yes;
| |
| - | mso-style-link:"Başlık 3";
| |
| - | mso-ansi-font-size:12.0pt;
| |
| - | mso-bidi-font-size:12.0pt;
| |
| - | font-family:"Calibri Light","sans-serif";
| |
| - | mso-ascii-font-family:"Calibri Light";
| |
| - | mso-ascii-theme-font:major-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:major-fareast;
| |
| - | mso-hansi-font-family:"Calibri Light";
| |
| - | mso-hansi-theme-font:major-latin;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | mso-bidi-theme-font:major-bidi;
| |
| - | color:#1F4D78;
| |
| - | mso-themecolor:accent1;
| |
| - | mso-themeshade:127;}
| |
| - | span.Balk4Char
| |
| - | {mso-style-name:"Başlık 4 Char";
| |
| - | mso-style-noshow:yes;
| |
| - | mso-style-priority:9;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-locked:yes;
| |
| - | mso-style-link:"Başlık 4";
| |
| - | mso-ansi-font-size:11.0pt;
| |
| - | mso-bidi-font-size:11.0pt;
| |
| - | font-family:"Calibri Light","sans-serif";
| |
| - | mso-ascii-font-family:"Calibri Light";
| |
| - | mso-ascii-theme-font:major-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:major-fareast;
| |
| - | mso-hansi-font-family:"Calibri Light";
| |
| - | mso-hansi-theme-font:major-latin;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | mso-bidi-theme-font:major-bidi;
| |
| - | color:#2E74B5;
| |
| - | mso-themecolor:accent1;
| |
| - | mso-themeshade:191;
| |
| - | font-style:italic;}
| |
| - | span.Balk5Char
| |
| - | {mso-style-name:"Başlık 5 Char";
| |
| - | mso-style-noshow:yes;
| |
| - | mso-style-priority:9;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-locked:yes;
| |
| - | mso-style-link:"Başlık 5";
| |
| - | mso-ansi-font-size:11.0pt;
| |
| - | mso-bidi-font-size:11.0pt;
| |
| - | font-family:"Calibri Light","sans-serif";
| |
| - | mso-ascii-font-family:"Calibri Light";
| |
| - | mso-ascii-theme-font:major-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:major-fareast;
| |
| - | mso-hansi-font-family:"Calibri Light";
| |
| - | mso-hansi-theme-font:major-latin;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | mso-bidi-theme-font:major-bidi;
| |
| - | color:#2E74B5;
| |
| - | mso-themecolor:accent1;
| |
| - | mso-themeshade:191;}
| |
| - | span.Balk6Char
| |
| - | {mso-style-name:"Başlık 6 Char";
| |
| - | mso-style-noshow:yes;
| |
| - | mso-style-priority:9;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-locked:yes;
| |
| - | mso-style-link:"Başlık 6";
| |
| - | mso-ansi-font-size:11.0pt;
| |
| - | mso-bidi-font-size:11.0pt;
| |
| - | font-family:"Calibri Light","sans-serif";
| |
| - | mso-ascii-font-family:"Calibri Light";
| |
| - | mso-ascii-theme-font:major-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:major-fareast;
| |
| - | mso-hansi-font-family:"Calibri Light";
| |
| - | mso-hansi-theme-font:major-latin;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | mso-bidi-theme-font:major-bidi;
| |
| - | color:#1F4D78;
| |
| - | mso-themecolor:accent1;
| |
| - | mso-themeshade:127;}
| |
| - | span.KonuBalChar
| |
| - | {mso-style-name:"Konu Başlığı Char";
| |
| - | mso-style-priority:10;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-locked:yes;
| |
| - | mso-style-link:"Konu Başlığı";
| |
| - | mso-ansi-font-size:28.0pt;
| |
| - | mso-bidi-font-size:28.0pt;
| |
| - | font-family:"Calibri Light","sans-serif";
| |
| - | mso-ascii-font-family:"Calibri Light";
| |
| - | mso-ascii-theme-font:major-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:major-fareast;
| |
| - | mso-hansi-font-family:"Calibri Light";
| |
| - | mso-hansi-theme-font:major-latin;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | mso-bidi-theme-font:major-bidi;
| |
| - | color:windowtext;
| |
| - | letter-spacing:-.5pt;
| |
| - | mso-font-kerning:14.0pt;}
| |
| - | p.msotitlecxspfirst, li.msotitlecxspfirst, div.msotitlecxspfirst
| |
| - | {mso-style-name:msotitlecxspfirst;
| |
| - | mso-style-unhide:no;
| |
| - | margin:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | page-break-after:avoid;
| |
| - | font-size:21.0pt;
| |
| - | font-family:"Trebuchet MS","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:minor-fareast;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | color:black;}
| |
| - | p.msotitlecxspmiddle, li.msotitlecxspmiddle, div.msotitlecxspmiddle
| |
| - | {mso-style-name:msotitlecxspmiddle;
| |
| - | mso-style-unhide:no;
| |
| - | margin:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | page-break-after:avoid;
| |
| - | font-size:21.0pt;
| |
| - | font-family:"Trebuchet MS","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:minor-fareast;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | color:black;}
| |
| - | p.msotitlecxsplast, li.msotitlecxsplast, div.msotitlecxsplast
| |
| - | {mso-style-name:msotitlecxsplast;
| |
| - | mso-style-unhide:no;
| |
| - | margin:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | page-break-after:avoid;
| |
| - | font-size:21.0pt;
| |
| - | font-family:"Trebuchet MS","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:minor-fareast;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | color:black;}
| |
| - | span.AltyazChar
| |
| - | {mso-style-name:"Altyazı Char";
| |
| - | mso-style-priority:11;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-locked:yes;
| |
| - | mso-style-link:Altyazı;
| |
| - | mso-ansi-font-size:11.0pt;
| |
| - | mso-bidi-font-size:11.0pt;
| |
| - | font-family:"Calibri","sans-serif";
| |
| - | mso-ascii-font-family:Calibri;
| |
| - | mso-ascii-theme-font:minor-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:minor-fareast;
| |
| - | mso-hansi-font-family:Calibri;
| |
| - | mso-hansi-theme-font:minor-latin;
| |
| - | mso-bidi-font-family:"Times New Roman";
| |
| - | mso-bidi-theme-font:minor-bidi;
| |
| - | color:#5A5A5A;
| |
| - | mso-themecolor:text1;
| |
| - | mso-themetint:165;
| |
| - | letter-spacing:.75pt;}
| |
| - | p.msosubtitlecxspfirst, li.msosubtitlecxspfirst, div.msosubtitlecxspfirst
| |
| - | {mso-style-name:msosubtitlecxspfirst;
| |
| - | mso-style-unhide:no;
| |
| - | margin:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
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| - | <p class=MsoNormal style='text-align:justify'><span style='font-size:16.0pt;
| |
| - | line-height:115%;font-family:"Calibri","sans-serif"'>CLONING THE TOP10 STRAINS</span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-size:16.0pt;
| |
| - | line-height:115%;font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-size:16.0pt;
| |
| - | line-height:115%;font-family:"Calibri","sans-serif"'>TRANSFORMATION</span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span class=SpellE><span
| |
| - | style='font-family:"Calibri","sans-serif"'>Plasmids</span></span><span
| |
| - | style='font-family:"Calibri","sans-serif"'> <span class=SpellE>containing</span>
| |
| - | <span class=SpellE>the</span> BBa_K1291071 (<span class=SpellE>Horseradish</span>
| |
| - | <span class=SpellE>peroxidase</span> <span class=SpellE>composite</span> <span
| |
| - | class=SpellE>part</span>) <span class=SpellE>and</span> BBa_K1291299 (Lignin <span
| |
| - | class=SpellE>Peroxidase</span> <span class=SpellE>composite</span> <span
| |
| - | class=SpellE>part</span>) <span class=SpellE>parts</span> <span class=SpellE>were</span>
| |
| - | <span class=SpellE>transferred</span> <span class=SpellE>into</span> <span
| |
| - | class=SpellE>our</span> T10 <span class=SpellE>strains</span>, <span
| |
| - | class=SpellE>which</span> <span class=SpellE>were</span> <span class=SpellE>prepared</span>
| |
| - | <span class=SpellE>through</span> <span class=SpellE>the</span> <span
| |
| - | class=SpellE>competent</span> <span class=SpellE>cell</span> <span
| |
| - | class=SpellE>preparation</span> <span class=SpellE>protocol</span>, <span
| |
| - | class=SpellE>using</span> <span class=SpellE>transformation</span> <span
| |
| - | class=SpellE>protocols</span>. <span class=SpellE>Colonies</span> <span
| |
| - | class=SpellE>were</span> <span class=SpellE>seen</span> in <span class=SpellE>agar</span>
| |
| - | <span class=SpellE>plates</span> <span class=SpellE>that</span> had <span
| |
| - | class=SpellE>the</span> <span class=SpellE>appropriate</span> <span
| |
| - | class=SpellE>antibiotics</span> <span class=SpellE>after</span> a 16 <span
| |
| - | class=SpellE>hour</span> <span class=SpellE>long</span> <span class=SpellE>incubation</span>
| |
| - | <span class=SpellE>process</span>. </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-size:16.0pt;
| |
| - | line-height:115%;font-family:"Calibri","sans-serif"'>PLASMID ISOLATION</span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'> <span
| |
| - | class=SpellE>The</span> <span class=SpellE>colonies</span> <span class=SpellE>taken</span>
| |
| - | <span class=SpellE>from</span> <span class=SpellE>plates</span> <span
| |
| - | class=SpellE>were</span> <span class=SpellE>incubated</span> in LB <span
| |
| - | class=SpellE>Broth</span> <span class=SpellE>containing</span> AMP <span
| |
| - | class=SpellE>for</span> 16 <span class=SpellE>hours</span>. DNA <span
| |
| - | class=SpellE>isolation</span> <span class=SpellE>was</span> <span class=GramE>done</span>
| |
| - | on <span class=SpellE>the</span> <span class=SpellE>bacteria</span> <span
| |
| - | class=SpellE>culture</span> <span class=SpellE>to</span> <span class=SpellE>check</span>
| |
| - | <span class=SpellE>the</span> <span class=SpellE>efficiency</span>. <span
| |
| - | class=SpellE>The</span> <span class=SpellE>adequacy</span> of <span
| |
| - | class=SpellE>the</span> <span class=SpellE>amount</span> of <span class=SpellE>the</span>
| |
| - | <span class=SpellE>nucleic</span> <span class=SpellE>acid</span> <span
| |
| - | class=SpellE>concentration</span> <span class=SpellE>for</span> <span
| |
| - | class=SpellE>digestion</span> <span class=SpellE>was</span> met in <span
| |
| - | class=SpellE>the</span> <span class=SpellE>acquired</span> <span class=SpellE>samples</span>
| |
| - | <span class=SpellE>and</span> it <span class=SpellE>was</span> <span
| |
| - | class=SpellE>seen</span> <span class=SpellE>that</span> <span class=SpellE>the</span>
| |
| - | <span class=SpellE>samples</span> had <span class=SpellE>peaks</span> at OD
| |
| - | 260, <span class=SpellE>which</span> <span class=SpellE>was</span> <span
| |
| - | class=SpellE>captured</span> <span class=SpellE>by</span> <span class=SpellE>the</span>
| |
| - | <span class=SpellE>nanodrop</span>.</span><span style='left:-20px;position:
| |
| - | relative;top:0px;z-index:251658240'> </p>
| |
| - |
| |
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| - | <td width=15 style='width:11.25pt;padding:0cm 0cm 0cm 0cm'></td>
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| - | <td width=290 style='width:217.5pt;padding:0cm 0cm 0cm 0cm'></td>
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| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span class=SpellE><span
| |
| - | style='font-size:16.0pt;line-height:115%;font-family:"Calibri","sans-serif"'>Digestion</span></span><span
| |
| - | style='font-size:16.0pt;line-height:115%;font-family:"Calibri","sans-serif"'> <span
| |
| - | class=SpellE>and</span> DNA <span class=SpellE>Electrophoresis</span></span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>Gel
| |
| - | <span class=SpellE>electrophoresis</span> <span class=SpellE>was</span> <span
| |
| - | class=SpellE>conducted</span> on <span class=SpellE>the</span> <span
| |
| - | class=SpellE>samples</span> <span class=SpellE>so</span> <span class=SpellE>that</span>
| |
| - | <span class=SpellE>the</span> <span class=SpellE>enzymes</span> <span
| |
| - | class=SpellE>which</span> <span class=SpellE>were</span> <span class=SpellE>digested</span>
| |
| - | <span class=SpellE>with</span> <span class=SpellE>the</span> <span
| |
| - | class=SpellE>EcoRI</span> <span class=SpellE>and</span> <span class=SpellE>PstI</span>
| |
| - | <span class=SpellE>could</span> be <span class=SpellE>verified</span> in <span
| |
| - | class=SpellE>terms</span> of <span class=SpellE>their</span> <span
| |
| - | class=SpellE>wavelength</span>. <span class=SpellE>The</span> <span
| |
| - | class=SpellE>acquired</span> <span class=SpellE>results</span> <span
| |
| - | class=SpellE>showed</span> <span class=SpellE>that</span> <span class=SpellE>the</span>
| |
| - | <span class=SpellE>plasmid</span> transfer <span class=SpellE>process</span> <span
| |
| - | class=SpellE>was</span> <span class=SpellE>successful</span> <span
| |
| - | class=SpellE>for</span> <span class=SpellE>the</span> Top10 <span class=SpellE>strains</span>
| |
| - | <span class=SpellE>while</span> <span class=SpellE>the</span> NEB10 <span
| |
| - | class=SpellE>strains</span>, <span class=SpellE>which</span> <span
| |
| - | class=SpellE>were</span> <span class=SpellE>taken</span> <span class=SpellE>from</span>
| |
| - | <span class=SpellE>the</span> <span class=SpellE>stock</span>, <span
| |
| - | class=SpellE>were</span> <span class=SpellE>disfunctional</span> <span
| |
| - | class=SpellE>thus</span> <span class=SpellE>were</span> <span class=SpellE>unusable</span>.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-size:16.0pt;
| |
| - | line-height:115%;font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-size:16.0pt;
| |
| - | line-height:115%;font-family:"Calibri","sans-serif"'>CLONING THE BL-21 STRAINS</span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>Since
| |
| - | <span class=SpellE>we</span> <span class=SpellE>require</span> a <span
| |
| - | class=SpellE>high</span> <span class=SpellE>concentration</span> of <span
| |
| - | class=SpellE>proteins</span> in <span class=SpellE>our</span> <span
| |
| - | class=SpellE>project</span>, <span class=SpellE>we</span> <span class=SpellE>decided</span>
| |
| - | <span class=SpellE>to</span> <span class=SpellE>use</span> <span class=SpellE>the</span>
| |
| - | BL-21 <span class=SpellE>strain</span> of <span class=SpellE>E.coli</span> <span
| |
| - | class=SpellE>instead</span> of Top10 <span class=SpellE>strains</span> <span
| |
| - | class=SpellE>due</span> <span class=SpellE>to</span> <span class=SpellE>its</span>
| |
| - | <span class=SpellE>enhanced</span> protein <span class=SpellE>synthesis</span> <span
| |
| - | class=SpellE>capacity</span>. <span class=SpellE>Plasmids</span> <span
| |
| - | class=SpellE>containing</span> <span class=SpellE>the</span> BBa_K1291071 (<span
| |
| - | class=SpellE>Horseradish</span> <span class=SpellE>peroxidase</span> <span
| |
| - | class=SpellE>composite</span> <span class=SpellE>part</span>) <span
| |
| - | class=SpellE>and</span> BBa_K1291299 (Lignin <span class=SpellE>Peroxidase</span>
| |
| - | <span class=SpellE>composite</span> <span class=SpellE>part</span>) <span
| |
| - | class=SpellE>parts</span> <span class=SpellE>were</span> <span class=SpellE>transferred</span>
| |
| - | <span class=SpellE>into</span> <span class=SpellE>our</span> BL-21 <span
| |
| - | class=SpellE>strains</span>, <span class=SpellE>which</span> <span
| |
| - | class=SpellE>were</span> <span class=SpellE>prepared</span> <span class=SpellE>through</span>
| |
| - | <span class=SpellE>the</span> <span class=SpellE>competent</span> <span
| |
| - | class=SpellE>cell</span> <span class=SpellE>preparation</span> <span
| |
| - | class=SpellE>protocol</span>, <span class=SpellE>using</span> <span
| |
| - | class=SpellE>the</span> <span class=SpellE>transformation</span> <span
| |
| - | class=SpellE>protocol</span>. <span class=SpellE>The</span> <span class=SpellE>colonies</span>
| |
| - | <span class=SpellE>successfully</span> <span class=SpellE>acquired</span> <span
| |
| - | class=SpellE>after</span> a 16-hour <span class=SpellE>long</span> <span
| |
| - | class=SpellE>incubation</span> <span class=SpellE>process</span>.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-size:16.0pt;
| |
| - | mso-bidi-font-size:11.0pt;line-height:115%;font-family:"Calibri","sans-serif"'>PLASMID
| |
| - | ISOLATION</span><span style='left:-20px;position:relative;top:0px;z-index:251662336'><span
| |
| - | style='font-size:16.0pt;mso-bidi-font-size:11.0pt;line-height:115%'> <o:p></o:p></span></p>
| |
| - |
| |
| - | <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0
| |
| - | style='mso-cellspacing:0cm;mso-yfti-tbllook:1184;mso-padding-alt:0cm 0cm 0cm 0cm'>
| |
| - | <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
| |
| - | <td width=0 style='width:.3pt;padding:0cm 0cm 0cm 0cm'></td>
| |
| - | <td width=309 style='width:231.75pt;padding:0cm 0cm 0cm 0cm'></td>
| |
| - | <td width=34 style='width:25.5pt;padding:0cm 0cm 0cm 0cm'></td>
| |
| - | <td width=281 style='width:210.75pt;padding:0cm 0cm 0cm 0cm'></td>
| |
| - | </tr>
| |
| - | <tr style='mso-yfti-irow:1;height:230.25pt'>
| |
| - | <td style='padding:0cm 0cm 0cm 0cm;height:230.25pt'></td>
| |
| - | <td rowspan=2 valign=top style='padding:0cm 0cm 0cm 0cm;height:230.25pt'>
| |
| - | <p class=MsoNormal><!--[if gte vml 1]><v:shape id="Resim_x0020_4" o:spid="_x0000_s1029"
| |
| - | type="#_x0000_t75" alt="https://static.igem.org/mediawiki/2014hs/1/1b/Cimage005.jpg"
| |
| - | style='position:absolute;margin-left:0;margin-top:0;width:231.75pt;height:231.75pt;
| |
| - | z-index:-251653120;visibility:visible;mso-wrap-style:square;
| |
| - | mso-wrap-distance-left:9pt;mso-wrap-distance-top:0;
| |
| - | mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0;
| |
| - | mso-position-horizontal:absolute;mso-position-horizontal-relative:text;
| |
| - | mso-position-vertical:absolute;mso-position-vertical-relative:text'>
| |
| - | <v:imagedata src="https://static.igem.org/mediawiki/2014hs/1/1b/Cimage005.jpg"/>
| |
| - | <w:wrap type="tight"/>
| |
| - | </v:shape><![endif]--><![if !vml]><img width=309 height=309
| |
| - | src="https://static.igem.org/mediawiki/2014hs/1/1b/Cimage005.jpg" align=left
| |
| - | hspace=12 alt="https://static.igem.org/mediawiki/2014hs/1/1b/Cimage005.jpg" v:shapes="Resim_x0020_4"><![endif]><span
| |
| - | style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif";
| |
| - | mso-fareast-font-family:"Times New Roman";color:windowtext'><o:p></o:p></span></p>
| |
| - | </td>
| |
| - | <td style='padding:0cm 0cm 0cm 0cm;height:230.25pt'></td>
| |
| - | <td valign=top style='padding:0cm 0cm 0cm 0cm;height:230.25pt'>
| |
| - | <p class=MsoNormal><!--[if gte vml 1]><v:shape id="Resim_x0020_5" o:spid="_x0000_s1028"
| |
| - | type="#_x0000_t75" alt="https://static.igem.org/mediawiki/2014hs/1/1f/Cimage006.jpg"
| |
| - | style='position:absolute;margin-left:0;margin-top:0;width:210.75pt;height:230.25pt;
| |
| - | z-index:-251652096;visibility:visible;mso-wrap-style:square;
| |
| - | mso-wrap-distance-left:9pt;mso-wrap-distance-top:0;
| |
| - | mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0;
| |
| - | mso-position-horizontal:absolute;mso-position-horizontal-relative:text;
| |
| - | mso-position-vertical:absolute;mso-position-vertical-relative:text'>
| |
| - | <v:imagedata src="https://static.igem.org/mediawiki/2014hs/1/1f/Cimage006.jpg"/>
| |
| - | <w:wrap type="tight"/>
| |
| - | </v:shape><![endif]--><![if !vml]><img width=281 height=307
| |
| - | src="https://static.igem.org/mediawiki/2014hs/1/1f/Cimage006.jpg" align=left
| |
| - | hspace=12 alt="https://static.igem.org/mediawiki/2014hs/1/1f/Cimage006.jpg" v:shapes="Resim_x0020_5"><![endif]><span
| |
| - | style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif";
| |
| - | mso-fareast-font-family:"Times New Roman";color:windowtext'><o:p></o:p></span></p>
| |
| - | </td>
| |
| - | </tr>
| |
| - | <tr style='mso-yfti-irow:2;mso-yfti-lastrow:yes;height:1.5pt'>
| |
| - | <td style='padding:0cm 0cm 0cm 0cm;height:1.5pt'></td>
| |
| - | <td style='padding:0cm 0cm 0cm 0cm;height:1.5pt'></td>
| |
| - | <td style='padding:0cm 0cm 0cm 0cm;height:1.5pt'></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><br clear=all style='mso-special-character:
| |
| - | line-break'>
| |
| - | </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><!--[if gte vml 1]><v:shape id="Resim_x0020_6"
| |
| - | o:spid="_x0000_s1027" type="#_x0000_t75" alt="https://static.igem.org/mediawiki/2014hs/5/55/Cimage007.gif"
| |
| - | style='position:absolute;left:0;text-align:left;margin-left:0;margin-top:1442.45pt;
| |
| - | width:567.75pt;height:362.25pt;z-index:-251651072;visibility:visible;
| |
| - | mso-wrap-style:square;mso-wrap-distance-left:9pt;mso-wrap-distance-top:0;
| |
| - | mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0;
| |
| - | mso-position-horizontal:left;mso-position-horizontal-relative:text;
| |
| - | mso-position-vertical:absolute;mso-position-vertical-relative:text'>
| |
| - | <v:imagedata src="https://static.igem.org/mediawiki/2014hs/5/55/Cimage007.gif"/>
| |
| - | <w:wrap type="tight"/>
| |
| - | </v:shape><![endif]--><![if !vml]><img width=757 height=483
| |
| - | src="https://static.igem.org/mediawiki/2014hs/5/55/Cimage007.gif" align=left
| |
| - | hspace=12 alt="https://static.igem.org/mediawiki/2014hs/5/55/Cimage007.gif" v:shapes="Resim_x0020_6"><![endif]><span
| |
| - | style='font-family:"Calibri","sans-serif"'><span
| |
| - | style='mso-spacerun:yes'>
| |
| - | </span><span style='mso-spacerun:yes'> </span><span
| |
| - | style='mso-spacerun:yes'> </span><span class=SpellE>The</span> <span
| |
| - | class=SpellE>chosen</span> <span class=SpellE>colonies</span> <span
| |
| - | class=SpellE>were</span> <span class=SpellE>incubated</span> <span
| |
| - | class=SpellE>for</span> 16-hours in AMP <span class=SpellE>containing</span> LB
| |
| - | <span class=SpellE>Broth</span>. DNA <span class=SpellE>Isolation</span> <span
| |
| - | class=SpellE>was</span> <span class=GramE>done</span> <span class=SpellE>to</span>
| |
| - | test <span class=SpellE>the</span> <span class=SpellE>efficiency</span> of <span
| |
| - | class=SpellE>the</span> <span class=SpellE>transformation</span>. A <span
| |
| - | class=SpellE>peek</span> at OD 260 <span class=SpellE>was</span> <span
| |
| - | class=SpellE>observed</span> <span class=SpellE>and</span> <span class=SpellE>sufficient</span>
| |
| - | <span class=SpellE>levels</span> of DNA <span class=SpellE>concentration</span>
| |
| - | <span class=SpellE>was</span> <span class=SpellE>acquired</span> <span
| |
| - | class=SpellE>to</span> <span class=SpellE>continue</span> <span class=SpellE>to</span>
| |
| - | <span class=SpellE>digestion</span>.</span><span style='left:-78px;position:
| |
| - | relative;top:0px;z-index:251664384'> <br clear=all style='mso-special-character:
| |
| - | line-break'>
| |
| - | <span style='font-family:"Calibri","sans-serif"'><o:p></o:p></span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><!--[if gte vml 1]><v:shape id="Resim_x0020_3"
| |
| - | o:spid="_x0000_s1026" type="#_x0000_t75" alt="https://static.igem.org/mediawiki/2014hs/c/cf/Cimage008.jpg"
| |
| - | style='position:absolute;left:0;text-align:left;margin-left:0;margin-top:0;
| |
| - | width:255pt;height:267.75pt;z-index:-251657216;visibility:visible;
| |
| - | mso-wrap-style:square;mso-width-percent:0;mso-height-percent:0;
| |
| - | mso-wrap-distance-left:9pt;mso-wrap-distance-top:9pt;
| |
| - | mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:9pt;
| |
| - | mso-position-horizontal:left;mso-position-horizontal-relative:text;
| |
| - | mso-position-vertical:absolute;mso-position-vertical-relative:line;
| |
| - | mso-width-percent:0;mso-height-percent:0;mso-width-relative:page;
| |
| - | mso-height-relative:page' o:allowoverlap="f">
| |
| - | <v:imagedata src="https://static.igem.org/mediawiki/2014hs/c/cf/Cimage008.jpg"/>
| |
| - | <w:wrap type="tight" anchory="line"/>
| |
| - | </v:shape><![endif]--><![if !vml]><img width=340 height=357
| |
| - | src="https://static.igem.org/mediawiki/2014hs/c/cf/Cimage008.jpg" align=left
| |
| - | hspace=12 vspace=12 alt="https://static.igem.org/mediawiki/2014hs/c/cf/Cimage008.jpg"
| |
| - | v:shapes="Resim_x0020_3"><![endif]><span class=SpellE><span style='font-size:
| |
| - | 16.0pt;line-height:115%;font-family:"Calibri","sans-serif"'>Digestion</span></span><span
| |
| - | style='font-size:16.0pt;line-height:115%;font-family:"Calibri","sans-serif"'> <span
| |
| - | class=SpellE>and</span> DNA <span class=SpellE>Electrophoresis</span></span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>Gel
| |
| - | <span class=SpellE>electrophoresis</span> <span class=SpellE>was</span> <span
| |
| - | class=SpellE>conducted</span> on <span class=SpellE>the</span> <span
| |
| - | class=SpellE>samples</span> <span class=SpellE>so</span> <span class=SpellE>that</span>
| |
| - | <span class=SpellE>the</span> <span class=SpellE>enzymes</span> <span
| |
| - | class=SpellE>which</span> <span class=SpellE>were</span> <span class=SpellE>digested</span>
| |
| - | <span class=SpellE>with</span> <span class=SpellE>the</span> <span
| |
| - | class=SpellE>EcoRI</span> <span class=SpellE>and</span> <span class=SpellE>PstI</span>
| |
| - | <span class=SpellE>could</span> be <span class=SpellE>verified</span> in <span
| |
| - | class=SpellE>terms</span> of <span class=SpellE>their</span> <span
| |
| - | class=SpellE>wavelength</span>. <span class=SpellE>The</span> <span
| |
| - | class=SpellE>acquired</span> <span class=SpellE>results</span> <span
| |
| - | class=SpellE>showed</span> <span class=SpellE>that</span> <span class=SpellE>the</span>
| |
| - | <span class=SpellE>plasmid</span> transfer <span class=SpellE>process</span> <span
| |
| - | class=SpellE>was</span> <span class=SpellE>successful</span> <span
| |
| - | class=SpellE>for</span> BL-21 <span class=SpellE>Strains</span> <span
| |
| - | class=SpellE>and</span> it <span class=SpellE>was</span> <span class=SpellE>possible</span>
| |
| - | <span class=SpellE>to</span> <span class=SpellE>continue</span> <span
| |
| - | class=SpellE>to</span> <span class=SpellE>the</span> <span class=SpellE>next</span>
| |
| - | step.</span></p>
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - | </body>
| |
| - |
| |
| - | </head>
| |
| - | </html>
| |
"
