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Team:Elan Vital South Korea/p process
From 2014hs.igem.org
Process
To investigate the multidrug resistance of MRSA, we first got a sample of MRSA from a local hospital. Then we extracted the DNA from the MRSA, and mixed them with the prepared E. coli, hopefully causing transformation. Now, the E. coli should show the same resistance to drugs the DNA from the original MRSA coded for. So, we can investigate the resistance of MRSA by attempting to grow the transformed E. coli in a culture of different antibiotics in different concentrations, and observing its growth. We tested the resistance of the transformed E. coli in different concentrations of ampicillin, gentamycin, kanamycin, and tetracycline. Then, to investigate the genes that were involved in the multidrug resistance, we first prepared the transformed E. coli via mini prep, and then ran the E. coli through PCR. This should multiply the number DNA in the E. coli, allowing investigation of the genes involved. Then we ran gel electrophoresis to investigate the genes involved.
