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From 2014hs.igem.org

Lambert iGEM NoteBook

Lab Notes
• Fall 2013: Our team visited a local farm to determine needs in our community. From the visit, we began formulating the idea of a fruit preservative.
• November and December 2013: We began to work on sterile techniques and safety training. Also began research into Chitin to Chitosan Pathway as well as applications of chitosan.
• January 2014: Began research into source organisms for the Chitin Deacytelase gene.
• February 2014: iGEM kit arrived.
• March 2014: Began extracting genomic yeast DNA. Used a protocol from Looke Article, Biotechnology Volume 50. using lithium acetate etc. Results were inconsistent and yields were low.
• April 2014: Used Pierce Thermoscientific Yeast DNA Extraction Kit to extract genomic DNA from S.cerevisiae. PCR using pMAL primers. Began Ligations.
• May 2014: Continued ligations using Quick Ligation, Overnight ligation and T4ligase. Further research determined low efficiency of blunt ended ligations.
• June 2014: Began Biobricking CDA gene. Designed Biobrick primers for CDA, PCR, digested, ligated with PSB1C3 and transformations with DH10beta competent cells.