Team:OLS Canmore AB CA/DIY.html

Link to download dremelfuge video if plug-in is missing: https://static.igem.org/mediawiki/2014hs/d/df/OLS_Diy_dremel_video.MOV

Since it has the ability to hold different attachments, the Dremel tool can be used for cutting, grinding, sanding, carving and polishing. Within our lab, its main purpose was a centrifuge. We ordered a 3D printed Dremelfuge piece (pictured left) to do this.

The Dremel tool has the capability of rotating at high and controlled speeds, a function that makes it a suitable centrifuge.

Dremelfuge concept on-line from:
http://www.thingiverse.com/thing:1483

Costs for dremelfuge (shipping, currency conversion and taxes included)
$67.06 - 3D dremelfuge bit from http://www.shapeways.com/
$176.09 – Dremel rotary tool and drill press stand from http://www.canadiantire.ca/en.html

We started off by purchasing a 4 x 8 foot particle board and cut it to pieces, creating 6 16x48 inch rectangles. We cut one of the the rectangles with the dimensions of 16x16, and another one in half to make the door and created a rectangular box by gluing them together and stapling them afterwards using a staple gun. We drilled 4 holes on one side being the top and this was where the platform was suspended with the use of fish lines. Next we drilled a hole on the 16x16” side to make a hole for the plugins.
We used a record player to simulate the same effect as a shaker table. We used cyanoacrylate and glued nuts to the spinning platform of the record player. We then used the corresponding bolt and drilled the a hole through the platform with the diameter just a bit bigger than the diameter of the bolt. 

With the box and the record player and platform assembled we just needed a heat source and a fan to dissipate the heat. We used a thermostat to set the temperature for the light bulb for the heat source. Initially we used computer fans to try and circulate the temperature. We ended up using a regular fan because the computer fans showed no progress.. 

However, because of fire hazards and the risk of mould growth, we had to choose different materials for the box. We ended up purchasing a plastic container with roughly the same dimensions. Since the box had a lid, we had to construct a frame to hang the fish wires that was later changed into chains. Since the record player was too big for everything to fit, we decided to strip all the unnecessary parts, (cd drive, lights, button mechanisms, etc.) and kept only the motor that spun the platform and the base. We then attached the base to the bottom of the plastic container through nuts and bolts. We attached the spinning platform with the motor using the same technique. However, since we did not have a power source for the motor, we used the 15V power supply to make it spin. We soldered one end of the alligator clips to the circuit board in order for everything to work. 

Air Temperature

Water Temperature

Fume Hood

Pipettes (p10, 20, 200, 1000)

Autoclave

Centrifuge - Bigger Tubes 2,300 rpm

PCR