Team:AUC TURKEY/Project/Parts

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  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 1"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" Name="Revision"/>
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  <w:LsdException Locked="false" Priority="34" QFormat="true"
 +
  Name="List Paragraph"/>
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  <w:LsdException Locked="false" Priority="29" QFormat="true" Name="Quote"/>
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  <w:LsdException Locked="false" Priority="30" QFormat="true"
 +
  Name="Intense Quote"/>
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  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 1"/>
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  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 1"/>
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  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 2"/>
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  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 6"/>
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  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 6"/>
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  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 6"/>
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  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 6"/>
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  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="19" QFormat="true"
 +
  Name="Subtle Emphasis"/>
 +
  <w:LsdException Locked="false" Priority="21" QFormat="true"
 +
  Name="Intense Emphasis"/>
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  <w:LsdException Locked="false" Priority="31" QFormat="true"
 +
  Name="Subtle Reference"/>
 +
  <w:LsdException Locked="false" Priority="32" QFormat="true"
 +
  Name="Intense Reference"/>
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  <w:LsdException Locked="false" Priority="33" QFormat="true" Name="Book Title"/>
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  <w:LsdException Locked="false" Priority="37" SemiHidden="true"
 +
  UnhideWhenUsed="true" Name="Bibliography"/>
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  <w:LsdException Locked="false" Priority="39" SemiHidden="true"
 +
  UnhideWhenUsed="true" QFormat="true" Name="TOC Heading"/>
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  <w:LsdException Locked="false" Priority="41" Name="Plain Table 1"/>
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  <w:LsdException Locked="false" Priority="42" Name="Plain Table 2"/>
 +
  <w:LsdException Locked="false" Priority="43" Name="Plain Table 3"/>
 +
  <w:LsdException Locked="false" Priority="44" Name="Plain Table 4"/>
 +
  <w:LsdException Locked="false" Priority="45" Name="Plain Table 5"/>
 +
  <w:LsdException Locked="false" Priority="40" Name="Grid Table Light"/>
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  <w:LsdException Locked="false" Priority="46" Name="Grid Table 1 Light"/>
 +
  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4"/>
 +
  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark"/>
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  <w:LsdException Locked="false" Priority="51" Name="Grid Table 6 Colorful"/>
 +
  <w:LsdException Locked="false" Priority="52" Name="Grid Table 7 Colorful"/>
 +
  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 1"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 1"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 1"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 1"/>
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  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 1"/>
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  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 1"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 2"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 2"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 2"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 2"/>
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  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 2"/>
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  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 2"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 3"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 3"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 3"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 3"/>
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  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 3"/>
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  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 3"/>
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  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 3"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 4"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 4"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 4"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 4"/>
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  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 4"/>
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  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 4"/>
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  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 4"/>
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  <w:LsdException Locked="false" Priority="46"
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  Name="Grid Table 1 Light Accent 5"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 5"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 5"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 5"/>
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  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 5"/>
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  <w:LsdException Locked="false" Priority="51"
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  Name="Grid Table 6 Colorful Accent 5"/>
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  <w:LsdException Locked="false" Priority="52"
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  Name="Grid Table 7 Colorful Accent 5"/>
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  <w:LsdException Locked="false" Priority="46"
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  Name="Grid Table 1 Light Accent 6"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 6"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 6"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 6"/>
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  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 6"/>
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  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 6"/>
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  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 6"/>
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  <w:LsdException Locked="false" Priority="46" Name="List Table 1 Light"/>
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  <w:LsdException Locked="false" Priority="47" Name="List Table 2"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3"/>
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  <w:LsdException Locked="false" Priority="49" Name="List Table 4"/>
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  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark"/>
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  <w:LsdException Locked="false" Priority="51" Name="List Table 6 Colorful"/>
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  <w:LsdException Locked="false" Priority="52" Name="List Table 7 Colorful"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="List Table 1 Light Accent 1"/>
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  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 1"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 1"/>
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  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 1"/>
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  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 1"/>
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  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="List Table 7 Colorful Accent 1"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="List Table 1 Light Accent 2"/>
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  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 2"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 2"/>
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  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 2"/>
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  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 2"/>
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  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 2"/>
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  <w:LsdException Locked="false" Priority="52"
 +
  Name="List Table 7 Colorful Accent 2"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="List Table 1 Light Accent 3"/>
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  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 3"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 3"/>
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  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 3"/>
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  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 3"/>
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  <w:LsdException Locked="false" Priority="51"
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  Name="List Table 6 Colorful Accent 3"/>
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  <w:LsdException Locked="false" Priority="52"
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  Name="List Table 7 Colorful Accent 3"/>
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  <w:LsdException Locked="false" Priority="46"
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  Name="List Table 1 Light Accent 4"/>
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  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 4"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 4"/>
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  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 4"/>
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  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 4"/>
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  <w:LsdException Locked="false" Priority="51"
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  Name="List Table 6 Colorful Accent 4"/>
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  <w:LsdException Locked="false" Priority="52"
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  Name="List Table 7 Colorful Accent 4"/>
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  <w:LsdException Locked="false" Priority="46"
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  Name="List Table 1 Light Accent 5"/>
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  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 5"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 5"/>
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  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 5"/>
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  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 5"/>
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  <w:LsdException Locked="false" Priority="51"
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  Name="List Table 6 Colorful Accent 5"/>
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  <w:LsdException Locked="false" Priority="52"
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  Name="List Table 7 Colorful Accent 5"/>
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  <w:LsdException Locked="false" Priority="46"
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  Name="List Table 1 Light Accent 6"/>
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  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 6"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 6"/>
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  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 6"/>
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  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 6"/>
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  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="List Table 7 Colorful Accent 6"/>
 +
</w:LatentStyles>
 +
</xml><![endif]-->
 +
<style>
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/* Font Definitions */
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@font-face
 +
{font-family:"Cambria Math";
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panose-1:2 4 5 3 5 4 6 3 2 4;
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mso-font-charset:1;
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mso-generic-font-family:roman;
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mso-font-format:other;
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mso-font-pitch:variable;
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mso-font-signature:0 0 0 0 0 0;}
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@font-face
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{font-family:Calibri;
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panose-1:2 15 5 2 2 2 4 3 2 4;
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mso-font-signature:-536870145 1073786111 1 0 415 0;}
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@font-face
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{font-family:Tahoma;
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panose-1:2 11 6 4 3 5 4 4 2 4;
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mso-font-charset:162;
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mso-generic-font-family:swiss;
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mso-font-pitch:variable;
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mso-font-signature:-520081665 -1073717157 41 0 66047 0;}
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/* Style Definitions */
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p.MsoNormal, li.MsoNormal, div.MsoNormal
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{mso-style-unhide:no;
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mso-style-qformat:yes;
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mso-style-parent:"";
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margin-top:0cm;
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margin-right:0cm;
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line-height:115%;
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mso-pagination:widow-orphan;
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font-family:"Calibri","sans-serif";
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mso-fareast-font-family:"Times New Roman";
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mso-fareast-theme-font:minor-fareast;
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mso-bidi-font-family:"Times New Roman";}
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p.MsoAcetate, li.MsoAcetate, div.MsoAcetate
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{mso-style-noshow:yes;
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mso-style-priority:99;
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mso-style-link:"Balon Metni Char";
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mso-fareast-font-family:"Times New Roman";
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span.BalonMetniChar
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{mso-style-name:"Balon Metni Char";
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<p class=MsoNormal><span lang=EN-US style='font-size:16.0pt;mso-bidi-font-size:
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11.0pt;line-height:115%;mso-ansi-language:EN-US'>Part Design:</span><span
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style='font-size:16.0pt;mso-bidi-font-size:11.0pt;line-height:115%'><o:p></o:p></span></p>
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 +
<p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>In our
 +
project there are 4 different parts. Two of them composite parts that are
 +
functional and other 2 parts are our enzymes that are proteins. We designed our
 +
parts with Gene Designer 2.0 program. We found our enzyme sequences from NCBI
 +
PubMed Database. In our project we need that our proteins to be produced and we
 +
need our enzymes so much because they do the all work. For more production we
 +
chose the strongest promoter there is which the strongest constitutive promoter
 +
J23119 in Part Registry is. When we design our parts we received this
 +
information that; scientists use inducible promoters for protein
 +
over-production so in our design, right after the promoter and also in front of
 +
our RBS, we added <span class=SpellE>BamHI</span> restriction enzyme site.
 +
After the RBS we added our enzymes sequences HRP-C and Lip-1 and after the
 +
enzymes sequences we added His-Tag <span class=GramE>( 6</span> Histidine
 +
sequences). Because we planned to do Western-Blotting technique in order to
 +
show that our proteins are produced successfully. &nbsp;</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>&nbsp;</span></p>
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<p class=MsoListParagraph style='text-indent:-18.0pt'><span lang=EN-US
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style='font-size:16.0pt;line-height:115%;mso-ansi-language:EN-US'>1)</span><span
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lang=EN-US style='font-size:7.0pt;line-height:115%;font-family:"Times New Roman","serif";
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mso-ansi-language:EN-US'>&nbsp;&nbsp;&nbsp; </span><span lang=EN-US
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style='font-size:16.0pt;line-height:115%;mso-ansi-language:EN-US'>Horseradish
 +
Peroxidase-C Part Design:</span></p>
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<p class=MsoListParagraph><span lang=EN-US style='mso-ansi-language:EN-US'>&nbsp;</span></p>
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<p class=MsoListParagraph><span style='mso-no-proof:yes'><img width=653
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<p class=MsoListParagraph style='text-indent:-18.0pt'><span lang=EN-US
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mso-ansi-language:EN-US'>&nbsp;&nbsp;&nbsp; </span><span lang=EN-US
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style='font-size:16.0pt;line-height:115%;mso-ansi-language:EN-US'>Lignin Peroxidase-1<span
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class=GramE>&nbsp; Part</span> Design : </span></p>
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Latest revision as of 17:35, 26 April 2015

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Part Design:

In our project there are 4 different parts. Two of them composite parts that are functional and other 2 parts are our enzymes that are proteins. We designed our parts with Gene Designer 2.0 program. We found our enzyme sequences from NCBI PubMed Database. In our project we need that our proteins to be produced and we need our enzymes so much because they do the all work. For more production we chose the strongest promoter there is which the strongest constitutive promoter J23119 in Part Registry is. When we design our parts we received this information that; scientists use inducible promoters for protein over-production so in our design, right after the promoter and also in front of our RBS, we added BamHI restriction enzyme site. After the RBS we added our enzymes sequences HRP-C and Lip-1 and after the enzymes sequences we added His-Tag ( 6 Histidine sequences). Because we planned to do Western-Blotting technique in order to show that our proteins are produced successfully.  

 

1)    Horseradish Peroxidase-C Part Design:

 

https://static.igem.org/mediawiki/2014hs/e/e7/1image001.png

 

2)    Lignin Peroxidase-1  Part Design :

                https://static.igem.org/mediawiki/2014hs/3/32/2image002.png

 

Retrieved from "http://2014hs.igem.org/Team:AUC_TURKEY/Project/Parts"