Team:TP CC-SanDiego
From 2014hs.igem.org
(Replaced content with "{{Team:TPHS/Main}} <html> <head> <link href="https://2013.igem.org/Team:Calgary/StyleSheets/HomePage?action=raw&ctype=text/css" rel="stylesheet"> </html>") |
|||
(61 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
- | {{Team: | + | {{Team:TP_CC-SanDiego/ContentFix}} |
- | + | {{Team:TP_CC-SanDiego/Main}} | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + |
Latest revision as of 22:44, 19 June 2014
Statistics that are quite relevant to the nature of this experiment
![](https://static.igem.org/mediawiki/2014/9/96/Toxic.png)
Toxicity of aflotoxin is 10 times that of hydrocyanic acid and 68 times of arsenic.
![](https://static.igem.org/mediawiki/2014/e/e4/Globe.png)
This disease is the third-leading cause of cancer death globally according to WHO (2008), with about 550,000–600,000 new cases each year.
![](https://static.igem.org/mediawiki/2014/8/84/Cow.png)
Mycotoxins affect nearly 15-20% of all crops.
E. Coli Capable of Extracelluar Secretion of Mycotoxin Detoxifying Enzymes
![](https://static.igem.org/mediawiki/2014/4/48/AbstractPic.png)
Microfungi produces harmful mycotoxins that flourish on improperly-stored nuts, grains, meat, and dairy. They especially thrive in developing countries, where the lack of advanced food storage and mycotoxin exposure cause 40% of the diseases. To lessen the problem, our team engineered E. coli strains using synthetic biology tools to produce chimeric mycotoxin-degrading fungal enzymes, Aflatoxin-Detoxifizyme (ADTZ) and Zearalenone Hydrolase (ZHD101), which are designed to secrete extracellularly by fusing with secretion signal peptides from alpha-amylase and beta-lactamase. In this study, we have successfully generated synthetic genetic materials to produce four chimeric mycotoxin-detoxifying enzymes. The levels of extracellular secretion is also characterized and analyzed. The project will allow a mass production of detoxification enzymes in a cost effective way, preventing the squandering of harvested crops, and limiting mycotoxin-related diseases. Increased access to these proteins will have immense commercial, industrial, health, and agricultural impacts.