Team:TP CC-SanDiego

From 2014hs.igem.org

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{{Team:TPHS/Main}}
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{{Team:TP_CC-SanDiego/Main}}
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<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #fe2b33; /*background-color: #fe2b33;*/">
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; /*color: #f6f6f6*/; padding: 5px;">
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This is a template page. READ THESE INSTRUCTIONS.
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</div>
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; /*color: #f6f6f6*/; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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</div>
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<div id="warning" style="/*text-align: center;*/ font-weight: bold; font-size: small; /*color: #f6f6f6*/; padding: 5px;">
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You <strong>MUST</strong> have the following information on your wiki:
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<ul style="font-weight:normal;">
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<li>a team description</li>
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<li>project description</li>
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<li>safety information (did your team take a safety training course? were you supervised in the lab?)</li>
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<li>team attribution (who did what part of your project?)</li>
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</ul>
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You may also wish to add other page such as:
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<ul style="font-weight:normal;">
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<li>lab notebook</li>
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<li>sponsor information</li>
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<li>other information</li>
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</ul>
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REMEMBER, keep all of your pages within your teams namespace. <br><span style="font-weight:normal; font-style:italic;">Example: 2013hs.igem.org/Team:TP_CC-SanDiego/Our_Pets</span>
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<!-- *********************** End of the alert box *********************** -->
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{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:TP_CC-SanDiego_logo.png|200px|right|frame]]
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|-
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:TP_CC-SanDiego_team.png|right|frame|Your team picture]]
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|-
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|align="center"|[[Team:TP_CC-SanDiego | Team TP_CC-SanDiego]]
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|}
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<!--- Team Information Link --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=TP_CC-SanDiego Official Team Profile]
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|}
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===Team===
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Tell us about your team, your school!
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===Project===
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What are you working on this semester?
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===Notebook===
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Show us how you spent your days.
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===Results/Conclusions===
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What did you achieve over the course of your semester?
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===Safety===
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What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
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===Attributions===
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Who worked on what?
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===Human Practices===
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What impact does/will your project have on the public? 
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===Fun!===
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What was your favorite team snack?? Have a picture of your team mascot?
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<forum_subtle />
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Latest revision as of 22:44, 19 June 2014

iGEM San Diego

Statistics
Statistics that are quite relevant to the nature of this experiment

Toxicity of aflotoxin is 10 times that of hydrocyanic acid and 68 times of arsenic.

This disease is the third-leading cause of cancer death globally according to WHO (2008), with about 550,000–600,000 new cases each year.

Mycotoxins affect nearly 15-20% of all crops.
Engineering
E. Coli Capable of Extracelluar Secretion of Mycotoxin Detoxifying Enzymes

Microfungi produces harmful mycotoxins that flourish on improperly-stored nuts, grains, meat, and dairy. They especially thrive in developing countries, where the lack of advanced food storage and mycotoxin exposure cause 40% of the diseases. To lessen the problem, our team engineered E. coli strains using synthetic biology tools to produce chimeric mycotoxin-degrading fungal enzymes, Aflatoxin-Detoxifizyme (ADTZ) and Zearalenone Hydrolase (ZHD101), which are designed to secrete extracellularly by fusing with secretion signal peptides from alpha-amylase and beta-lactamase. In this study, we have successfully generated synthetic genetic materials to produce four chimeric mycotoxin-detoxifying enzymes. The levels of extracellular secretion is also characterized and analyzed. The project will allow a mass production of detoxification enzymes in a cost effective way, preventing the squandering of harvested crops, and limiting mycotoxin-related diseases. Increased access to these proteins will have immense commercial, industrial, health, and agricultural impacts.

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