Team:TP CC-SanDiego

From 2014hs.igem.org

(Difference between revisions)
 
(25 intermediate revisions not shown)
Line 1: Line 1:
-
{{Team:TPHS/Main}}
+
{{Team:TP_CC-SanDiego/ContentFix}}
-
{{Team:TPHS/ContentFix}}
+
{{Team:TP_CC-SanDiego/Main}}
-
<html>
+
-
<style>
+
-
/*CSS for Slider
+
-
#HomePageContent {
+
-
position: relative;
+
-
top: 108px;
+
-
}
+
-
 
+
-
#Banner {
+
-
display: none;
+
-
}
+
-
 
+
-
/* Slider Styles */
+
-
#Slider {
+
-
position: relative;
+
-
width: 100%;
+
-
display: none;
+
-
}
+
-
 
+
-
#Slider .Slides {
+
-
width: 100%;
+
-
height: 100%;
+
-
background-size: 100%, auto;
+
-
}
+
-
 
+
-
#Slider #Slide0 {
+
-
background-image: url(https://static.igem.org/mediawiki/2014hs/4/4d/SliderIntro.png);
+
-
}
+
-
 
+
-
#Slider #Slide1 {
+
-
background-image: url();
+
-
display: none;
+
-
}
+
-
 
+
-
#Slider #Slide2 {
+
-
background-image: url();
+
-
display: none;
+
-
}
+
-
 
+
-
#Slider #Slide3 {
+
-
background-image: url();
+
-
display: none;
+
-
}
+
-
 
+
-
#Slider #Slide4 {
+
-
background-image: url();
+
-
display: none;
+
-
}
+
-
 
+
-
#Slider #Slide5 {
+
-
background-image: url();
+
-
display: none;
+
-
}
+
-
 
+
-
#Slider #BottomLinks {
+
-
position: absolute;
+
-
width: 100%;
+
-
padding: 0;
+
-
bottom: 0;
+
-
background: #353132;
+
-
text-align: center;
+
-
opacity: 0.61;
+
-
filter: alpha(opacity=61);
+
-
}
+
-
 
+
-
#Slider #BottomLinks li {
+
-
display: inline-table;
+
-
list-style: none;
+
-
height: 100%;
+
-
color: #FFFFFF;
+
-
font: 24px Raleway, Arial, san-serif;
+
-
text-align: center;
+
-
padding: 15px 20px;
+
-
}
+
-
 
+
-
#Slider #BottomLinks li:hover {
+
-
cursor: pointer;
+
-
text-decoration: underline;
+
-
}
+
-
 
+
-
#Slider .SideBar {
+
-
position: absolute;
+
-
top: 0;
+
-
width: 90px;
+
-
z-index: 1;
+
-
}
+
-
 
+
-
#Slider #LeftBar {
+
-
left: 0;
+
-
}
+
-
 
+
-
#Slider #RightBar {
+
-
right: 0;
+
-
}
+
-
 
+
-
#Slider #LeftArrow {
+
-
position: absolute;
+
-
width: 60px;
+
-
height: 60px;
+
-
margin-left: 30px;
+
-
background: url(https://static.igem.org/mediawiki/2014hs/2/22/SliderLeft.png) top no-repeat;
+
-
}
+
-
 
+
-
#Slider #RightArrow {
+
-
position: absolute;
+
-
width: 60px;
+
-
height: 60px;
+
-
margin-right: 30px;
+
-
background: url(https://static.igem.org/mediawiki/2014hs/d/d5/SliderRight.png) top no-repeat;
+
-
}
+
-
 
+
-
#Slider #LeftArrow:hover, #Slider #RightArrow:hover {
+
-
background-position: bottom;
+
-
cursor: pointer;
+
-
}
+
-
 
+
-
/* Other Styles */
+
-
#Overview {
+
-
width: 100%;
+
-
background: #E7E7E7;
+
-
padding: 25px 7.5% 40px 5%;
+
-
box-shadow: inset 0 0 20px 5px #888888;
+
-
text-align: left;
+
-
}
+
-
 
+
-
#Overview p {
+
-
margin: 18px 0 0 30px;
+
-
text-indent: 50px;
+
-
}
+
-
 
+
-
#Overview i, #Overview em, #Overview b, #Overview strong {
+
-
font-size: 18px;
+
-
}
+
-
 
+
-
#SectionLinks {
+
-
overflow: hidden;
+
-
width: 100%;
+
-
background: #383132;
+
-
margin: 0 auto;
+
-
padding: 10px;
+
-
text-align: center;
+
-
}
+
-
 
+
-
#SectionLinks a:hover {
+
-
text-decoration: none;
+
-
}
+
-
 
+
-
#SectionLinks .DescriptionLink {
+
-
float: left;
+
-
margin: 3%;
+
-
height: 400px;
+
-
width: 440px;
+
-
}
+
-
 
+
-
#SectionLinks .DescriptionLink h2 {
+
-
margin: 0;
+
-
}
+
-
 
+
-
#SectionLinks .Clear {
+
-
clear: both;
+
-
}
+
-
 
+
-
#SectionLinks .Wrap {
+
-
margin: auto;
+
-
min-width: 270px;
+
-
max-width: 1000px;
+
-
display: block;
+
-
margin: auto;
+
-
border: 0;
+
-
padding: 0;
+
-
}
+
-
 
+
-
#SectionLinks .DescriptionLink {
+
-
background: #4F494A;
+
-
padding: 20px;
+
-
position: relative;
+
-
}
+
-
 
+
-
#SectionLinks .DescriptionLink p {
+
-
padding-left: 0;
+
-
text-indent: 0;
+
-
position: absolute;
+
-
bottom: 10px;
+
-
padding-right: 20px;
+
-
}
+
-
 
+
-
#SectionLinks #DescriptionLink1 {
+
-
background: #299D77;
+
-
}
+
-
 
+
-
#SectionLinks #DescriptionLink1:hover {
+
-
background: #53B092;
+
-
}
+
-
 
+
-
#SectionLinks #DescriptionLink2 {
+
-
background: #87CA7A;
+
-
}
+
-
 
+
-
#SectionLinks #DescriptionLink2:hover {
+
-
background: #9FD494;
+
-
}
+
-
 
+
-
#SectionLinks #DescriptionLink3 {
+
-
background: #E68930;
+
-
}
+
-
 
+
-
#SectionLinks #DescriptionLink3:hover {
+
-
background: #EBA059;
+
-
}
+
-
 
+
-
#SectionLinks #DescriptionLink4 {
+
-
background: #EDB74B;
+
-
}
+
-
 
+
-
#SectionLinks #DescriptionLink4:hover {
+
-
background: #F0C56E;
+
-
}
+
-
 
+
-
h1, h2, h3 {
+
-
font-family: 'Raleway', Helvetica, Arial, sans-serif;
+
-
line-height: 1.6;
+
-
text-align: left;
+
-
}
+
-
 
+
-
h1 {
+
-
font-size: 35px;
+
-
font-weight: 700;
+
-
border-bottom: 3px solid #5A5A5A;
+
-
color: #111111;
+
-
margin: 0 -20px;
+
-
padding-left: 20px;
+
-
}
+
-
 
+
-
h2 {
+
-
font-size: 28px;
+
-
font-weight: 600;
+
-
color: #333333;
+
-
margin-top: 30px;
+
-
}
+
-
 
+
-
h3 {
+
-
font-size: 26px;
+
-
font-weight: 500;
+
-
color: #333333;
+
-
padding-left: 20px;
+
-
}
+
-
 
+
-
p {
+
-
font: 18px/1.6 'Segoe UI', Calibri, Helvetica, sans-serif;
+
-
margin: 4px 0 12px 0;
+
-
padding-left: 20px;
+
-
text-indent: 50px;
+
-
color: #000000;
+
-
text-align:justify;
+
-
text-justify:inter-word;
+
-
}
+
-
 
+
-
p i {
+
-
font-size: 18px;
+
-
}
+
-
</style>
+
-
<script>
+
-
$(document).ready(function() {
+
-
//Display slider for Javascript users
+
-
$('#Slider').css('display', 'block');
+
-
+
-
//Set height of slider (16/5 ratio)
+
-
$('#Slider').css('height', ($('body').width() / 3.2));
+
-
+
-
//Position right/left arrows
+
-
var BarHeight = $('#Slider').height() -  $('#BottomLinks').height();
+
-
$('#LeftBar, #RightBar').css('height', BarHeight);
+
-
$('#LeftArrow, #RightArrow').css('top', (BarHeight-60)/2);
+
-
 
+
-
//Repeats for window resize
+
-
$(window).resize(function() {
+
-
$('#Slider').css('height', ($('body').width() / 3.2));
+
-
+
-
var BarHeight = $('#Slider').height() -  $('#BottomLinks').height();
+
-
$('#LeftBar, #RightBar').css('height', BarHeight);
+
-
$('#LeftArrow, #RightArrow').css('top', (BarHeight-60)/2);
+
-
});
+
-
+
-
var TotalSlides = $('.Slides').length;
+
-
var CurrentSlide = 0;
+
-
 
+
-
$('#RightArrow').click(function() {
+
-
CurrentSlide = ++CurrentSlide % TotalSlides;
+
-
changeSlide(CurrentSlide);
+
-
});
+
-
+
-
$('#LeftArrow').click(function() {
+
-
CurrentSlide = (CurrentSlide + TotalSlides - 1) % TotalSlides;
+
-
changeSlide(CurrentSlide);
+
-
});
+
-
+
-
$('.SlideLink').click(function() {
+
-
var ClickedSlide = $(this).attr('id');
+
-
CurrentSlide = parseInt(ClickedSlide.replace('Link', ''));
+
-
changeSlide(CurrentSlide);
+
-
});
+
-
+
-
function changeSlide(num){
+
-
var SlideID = '#Slide' + num;
+
-
$('.Slides').css('display', 'none');
+
-
$(SlideID).css('display', 'block');
+
-
}
+
-
});
+
-
</script>
+
-
</head>
+
-
+
-
<body>
+
-
<section id="HomePageContent">
+
-
<div id="Slider">
+
-
<div id="Slide0" class="Slides current"></div>
+
-
<div id="Slide1" class="Slides"></div>
+
-
<div id="Slide2" class="Slides"></div>
+
-
<div id="Slide3" class="Slides"></div>
+
-
+
-
<div id="LeftBar" class="SideBar"><div id="LeftArrow"></div></div>
+
-
<div id="RightBar" class="SideBar"><div id="RightArrow"></div></div>
+
-
<ul id="BottomLinks">
+
-
<li id="Link0" class="SlideLink">Introduction</li>
+
-
                                        <li id="Link1" class="SlideLink">The Situation</li>
+
-
                                        <li id="Link2" class="SlideLink">The Problem</li>
+
-
                                        <li id="Link3" class="SlideLink">The Solution</li>
+
-
                                     
+
-
</ul>
+
-
</div>
+
-
<
+
-
<section id="Content">
+
-
<h1>Please check our Project tab for our Project Description.</h1>
+
-
 
+
-
</html>
+

Latest revision as of 22:44, 19 June 2014

iGEM San Diego

Statistics
Statistics that are quite relevant to the nature of this experiment

Toxicity of aflotoxin is 10 times that of hydrocyanic acid and 68 times of arsenic.

This disease is the third-leading cause of cancer death globally according to WHO (2008), with about 550,000–600,000 new cases each year.

Mycotoxins affect nearly 15-20% of all crops.
Engineering
E. Coli Capable of Extracelluar Secretion of Mycotoxin Detoxifying Enzymes

Microfungi produces harmful mycotoxins that flourish on improperly-stored nuts, grains, meat, and dairy. They especially thrive in developing countries, where the lack of advanced food storage and mycotoxin exposure cause 40% of the diseases. To lessen the problem, our team engineered E. coli strains using synthetic biology tools to produce chimeric mycotoxin-degrading fungal enzymes, Aflatoxin-Detoxifizyme (ADTZ) and Zearalenone Hydrolase (ZHD101), which are designed to secrete extracellularly by fusing with secretion signal peptides from alpha-amylase and beta-lactamase. In this study, we have successfully generated synthetic genetic materials to produce four chimeric mycotoxin-detoxifying enzymes. The levels of extracellular secretion is also characterized and analyzed. The project will allow a mass production of detoxification enzymes in a cost effective way, preventing the squandering of harvested crops, and limiting mycotoxin-related diseases. Increased access to these proteins will have immense commercial, industrial, health, and agricultural impacts.