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Team:TP CC-SanDiego
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Revision as of 17:27, 19 June 2014
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<img src="images/front page diagram white 3.png" width="400px" height="400px"/>
<a href="#header-text">See Statistics</a>
STATISTICS
Statistics that are quite relevant to the nature of this experiment
<img src="images/600px-White_Globe_Icon.png" width="150px" height="150px" />
Toxicity of aflotoxin is 10 times that of hydrocyanic acid and 68 times of arsenic
<img src="images/600px-White_Globe_Icon.png" width="150px" height="150px" />
This disease is the third-leading cause of cancer death globally according to WHO (2008), with about 550,000–600,000 new cases each year.
<img src="images/600px-White_Globe_Icon.png" width="150px" height="150px" />
This disease is the third-leading cause of cancer death globally according to WHO (2008), with about 550,000–600,000 new cases each year
Engineering
E. Coli Capable of Extracelluar Secretion of Mycotoxin Detoxifying Enzymes
<img src="images/bannerphase4 transparent white text.png" width="1070" height="292"/>
Microfungi that produce harmful mycotoxins flourish on improperly-stored nuts, grains, meat, and dairy. They especially thrive in developing countries, where the lack of advanced food storage and mycotoxin exposure causes 40% of the diseases. To lessen the problem, our team engineered E. coli strains using synthetic biology tools to produce chimeric mycotoxin-degrading fungal enzymes, Aflatoxin-Detoxifizyme (ADTZ) and Zearalenone Hydrolase (ZHD101), which are designed to be secreted to extra-cellular space by fusing with secretion signal peptides from alpha-amylase and beta-lactamase. In this study, we have successfully generated synthetic genetic materials to produce four chimeric mycotoxin-detoxifying enzymes. The levels of extracellular secretion is also characterized and analyzed. The project will allow a mass production of detoxification enzymes in cost effective way, preventing the squandering of harvested crops, and limiting mycotoxin-related diseases. Increased access to th ese proteins will have an immense commercial, industrial, agricultural, and health impact.
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