Team:Shenzhen SFLS

From 2014hs.igem.org

(Difference between revisions)
(Project Description)
(Killing Aspergillus flavus)
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=====Killing Aspergillus flavus=====
=====Killing Aspergillus flavus=====
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Bacillus subtilis will increase its output of iturin A (something kills Aspergillus flavus) drastically after being inplanted the engineered circuit. Therefore, this process greatly improves the efficiency of the delimitation of Aspergillus flavus.  
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Bacillus subtilis will increase its output of surfactin (something kills Aspergillus flavus) drastically after being inplanted the engineered circuit. Therefore, this process greatly improves the efficiency of the delimitation of Aspergillus flavus.
=====Neutralizing Aflatoxin B1=====
=====Neutralizing Aflatoxin B1=====

Revision as of 12:34, 6 March 2014


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have the following information on your wiki:
  • a team description
  • project description
  • safety information (did your team take a safety training course? were you supervised in the lab?)
  • team attribution (who did what part of your project?)
You may also wish to add other page such as:
  • lab notebook
  • sponsor information
  • other information
REMEMBER, keep all of your pages within your teams namespace.
Example: 2013hs.igem.org/Team:Shenzhen_SFLS/Our_Pets



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Shenzhen SFLS logo.png

Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)

File:Shenzhen SFLS team.png
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Team Shenzhen_SFLS


Official Team Profile

Contents

Team

Tell us about your team, your school!


Project

Project Description

Shenzhen_SFLS 2014 project description

To begin with

Aflatoxin B1 generated by Aspergillus flavus is a kind of lethal toxin that always contaminates the grains we eat. Aimed to minimize the harm, our project consists of 3 parts from different aspects: killing Aspergillus flavus, neutralizing aflatoxin B1, and reducing the virulence of aflatoxin B1 by turning it into weaker aflatoxin M1.

Killing Aspergillus flavus

Bacillus subtilis will increase its output of surfactin (something kills Aspergillus flavus) drastically after being inplanted the engineered circuit. Therefore, this process greatly improves the efficiency of the delimitation of Aspergillus flavus.

Neutralizing Aflatoxin B1

By optimizing the circuit, aflatoxin will be combined with certain antibodies, and further be immobilized on E.coli’s membrane protein.

Transforming toxic aflatoxin B1 into aflatoxin M1

It will produce astaxanthin to transform fatal aflatoxin B1 into less toxic aflatoxin M1 after its circuit is constructed. In this way, those toxicated grains would be detoxified and would even reach edible standard. We are now trying to improve the yield of astaxant.

In conclusion

As long as the plan is carried out smoothly, three circuits would be connected into a system. The system will start to produce iturin-A automatically once aflatoxin B1 is detected. At the same time, it will trigger E.coli to produce the antibody. Once the antibody is immobilized on the membrane protein, astaxanthin will start to be generated by. As a result, aflatoxin B1 is transformed into aflatoxin M1.

If our project is carried out successfully and is applied in real life, humanbeings would benefit from it to a great extent. In fact, aflatoxin contamination in agriculture and animal husbandry causes a loss of hundreds of million dollars per year. Despite the harsh current situation, we still believe that our relentless effort will bring the problem to an end. The contaminated grains would be detoxified until they are eligible. To be optimistic, many problems the whole population face about human health such as the storage of grains, the food's safety and medical hygiene problems would be solved by our project.

Notebook

Show us how you spent your days.


Results/Conclusions

What did you achieve over the course of your semester?


Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?


Attributions

Who worked on what?


Human Practices

What impact does/will your project have on the public?


Fun!

What was your favorite team snack?? Have a picture of your team mascot?


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