Team:SMTexas/Notebook

From 2014hs.igem.org

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==Notebook==
==Notebook==
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On Tuesday, February 18th, Vikrant R, Rohin M, and Gopal R, inoculated bacterial strains A & B onto LB agar with ampicillin & kanamycin and incubated overnight at 37C.
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DEVELOPMENTAL PHASE
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WEEK OF MARCH 17
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On Wednesday, February 19th, Vikrant R & Rohin M transferred one colony from each of A & B and inoculated in LB BROTH with ampicillin & kanamycin and placed in shaking water bath at 37C.
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General Meeting: Captain introduces details of iGEM to new members, reveals the project direction to all members, and introduces the creation/makeup of the Committees (30 min), divide the team into groups, have first group meetings, discuss having an additional Tuesday or Wednesday after school meetin
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The next step is to perform a miniprep, which will allow us to extract plasmid from each A & B bacterial strains, which will then allow us do the restriction digest and ligation.  
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Fundamental Research: Go over http://parts.igem.org/Help:Learn, the folder "Learn" in Dropbox
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All of these steps can be seen in the 3A Assembly kit protocols. Further research progress will be added soon.
+
 +
Applied Research: Organize and store DNA Distribution kit materials, (time permitting) go over pipetting with Mr. Adame
 +
 +
Outreach: brainstorm ideas about getting more Marksmen interested in iGEM, plan to make a brochure, determine a date to display the posters Killian created around the campus
 +
 +
Web Development: go over wiki design/layout, ask Mr. Rummel if you all are having trouble
 +
 +
 +
WEEK OF MARCH 24
 +
 +
General Meeting: Captain introduces Gene Designer (20 min)
 +
 +
Fundamental Research:  Start designing in Gene Designer by looking at possible promoters & repressors, peruse: http://www.biotechniques.com/news/University-of-Cambridge-team-wins-iGEM-synthetic-biology-competition/biotechniques-180278.html & http://biospot10.blogspot.com/2011/07/igem-grand-prize-2009-biosensors-and.html
 +
 +
Applied Research: Work with the Fundamental Research group to develop a streamlined construct, go over best practices in the lab with Mr. Adame
 +
 +
Outreach: start posting posters, print out 300-400 copies of the brochure depending on cost, plan to distribute the brochures after Friday's Upper School Assembly (April 11)
 +
 +
Web Development: follow iGEM Wiki guidelines and create relevant content, make sure to work on the team page
 +
 +
 +
WEEK OF MARCH 31
 +
 +
General Meeting: presentation by the Fundamental Research Group on their findings & in-development construct
 +
 +
Fundamental Research: work with the Applied Research Group to start constructing the plasmid, continue research
 +
Applied Research: find protocols for implementing the construct, identify problems and consult the Fundamental Research Group
 +
Outreach: brainstorm ideas on promoting the biotechnology further, esp. ideas for getting additional funding, possibly think about hosting parents for a luncheon, e-mail Dr. Burma and any other professors at UTSW for lab tour/advice/guidance
 +
Web Development: document progress and work with the Outreach Group
 +
 +
POSTPONED B/C of ISAS Mandatory Meeting: APRIL 2 Evaluation: Meeting with Mr. Adame, Mr. Rummel, and Committee Leaders
 +
 +
ISAS Commitments will be taken into consideration
 +
 +
CONSTRUCTION PHASE
 +
 +
WEEK OF APRIL 7
 +
 +
General Meeting: Captain speech on the leadership policy, Evaluation of Committees by Captain/Faculty Sponsors
 +
 +
Fundamental Research: Continue research
 +
Applied Research: 3A Assembly
 +
Outreach: Finalize Brochure
 +
Web Development: Continue work on the wiki
 +
 +
Captain seeks new recruits for next year's iGEM Team; reveals ultimate vision of the Project to the Upper School; sends the Biotechnology Initiative brochure to underclassmen
 +
 +
WEEK OF APRIL 14
 +
 +
General Meeting: Committee Meetings
 +
 +
Fundamental Research: Finalize research on genes for the detection of VOC biomarkers
 +
Applied Research: Work with the Fundamental Research Team on finding the DNA sequences, reinforce laboratory skills, calculate potential budget for future years
 +
Outreach: Posters around campus, print out 200 copies of the brochure and distribute to interested science instructors, begin development of a more formal packet of info for the administration
 +
Web Development: Assist Outreach, finalize at least half of the wiki
 +
 +
CANCELED UNTIL CONSTRUCT DEVELOPMENT IS COMPLETE: Plan for a UTSW visit April 18
 +
 +
WEEK OF APRIL 21
 +
 +
General Meeting: Brief presentation by Web Development on Thursday
 +
 +
Fundamental Research: Begin creating construct in Gene Designer if necessary, look for options for the purchase of these sequences from biotech companies, check iGEM Registry for existing BioBricks
 +
Applied Research: Finalize budget, continue refining laboratory skills, assist the Fundamental Research Team
 +
Outreach: Finalize packet for the admininstration, print 10 color copies
 +
Web Development: Finalize wiki with detailed content & designs, brief presentation to the Team on how to add/modify content
 +
 +
WEEK OF APRIL 28
 +
 +
General Meeting: Presentation on established construct by the Fundamental Research Team
 +
 +
Fundamental Research: Order sequences, collaborate with the Applied Research Team to address possible issues during the actual creation of the construct
 +
Applied Research: Collaborate with the Fundamental Research Team to identify room for error
 +
Outreach: Design iGEM wearable gear (t-shirt, sweatshirt, and/or hat) and list cost in a document
 +
Web Development: Address iGEM requirements, including Human Practices
 +
 +
WEEK OF MAY 5: AP EXAMINATIONS
 +
 +
General Meeting:
 +
 +
Fundamental Research:
 +
Applied Research:
 +
Outreach:
 +
Web Development:
 +
 +
WEEK OF MAY 12: AP EXAMINATIONS
 +
 +
General Meeting:
 +
 +
Fundamental Research:
 +
Applied Research:
 +
Outreach:
 +
Web Development:
 +
 +
WEEK OF MAY 19
 +
 +
General Meeting:
 +
 +
Fundamental Research:
 +
Applied Research:
 +
Outreach:
 +
Web Development:
 +
 +
WEEK OF MAY 26
 +
 +
General Meeting: Evaluate progress
 +
 +
MAY 30: REGULAR REGISTRATION FOR HIGH SCHOOL JAMBOREE CLOSES, JAMOREE ATTENDANCE FEES DUE
 +
 +
WEEK OF JUNE 2
 +
 +
General Meeting: Evaluate progress
 +
 +
JUNE 8: TRACK SELECTION DUE, PROJECT ABSTRACTS DUE, TEAM ROSTERS DUE
 +
 +
WEEK OF JUNE 9
 +
 +
General Meeting: Evaluate progress
 +
 +
 +
 +
WEEK OF JUNE 16
 +
 +
 +
 +
June 20: Wiki Freeze at 11:59PM Boston time (see: wiki requirements and safety questions)
 +
 +
WEEK OF JUNE 23
 +
 +
Practice presentation, polish poster, submit part to iGEM
 +
 +
 +
JUNE 28: JAMBOREE!

Revision as of 23:04, 1 May 2014

Notebook

DEVELOPMENTAL PHASE WEEK OF MARCH 17

General Meeting: Captain introduces details of iGEM to new members, reveals the project direction to all members, and introduces the creation/makeup of the Committees (30 min), divide the team into groups, have first group meetings, discuss having an additional Tuesday or Wednesday after school meetin

Fundamental Research: Go over http://parts.igem.org/Help:Learn, the folder "Learn" in Dropbox

Applied Research: Organize and store DNA Distribution kit materials, (time permitting) go over pipetting with Mr. Adame

Outreach: brainstorm ideas about getting more Marksmen interested in iGEM, plan to make a brochure, determine a date to display the posters Killian created around the campus

Web Development: go over wiki design/layout, ask Mr. Rummel if you all are having trouble


WEEK OF MARCH 24

General Meeting: Captain introduces Gene Designer (20 min)

Fundamental Research: Start designing in Gene Designer by looking at possible promoters & repressors, peruse: http://www.biotechniques.com/news/University-of-Cambridge-team-wins-iGEM-synthetic-biology-competition/biotechniques-180278.html & http://biospot10.blogspot.com/2011/07/igem-grand-prize-2009-biosensors-and.html

Applied Research: Work with the Fundamental Research group to develop a streamlined construct, go over best practices in the lab with Mr. Adame

Outreach: start posting posters, print out 300-400 copies of the brochure depending on cost, plan to distribute the brochures after Friday's Upper School Assembly (April 11)

Web Development: follow iGEM Wiki guidelines and create relevant content, make sure to work on the team page


WEEK OF MARCH 31

General Meeting: presentation by the Fundamental Research Group on their findings & in-development construct

Fundamental Research: work with the Applied Research Group to start constructing the plasmid, continue research Applied Research: find protocols for implementing the construct, identify problems and consult the Fundamental Research Group Outreach: brainstorm ideas on promoting the biotechnology further, esp. ideas for getting additional funding, possibly think about hosting parents for a luncheon, e-mail Dr. Burma and any other professors at UTSW for lab tour/advice/guidance Web Development: document progress and work with the Outreach Group

POSTPONED B/C of ISAS Mandatory Meeting: APRIL 2 Evaluation: Meeting with Mr. Adame, Mr. Rummel, and Committee Leaders

ISAS Commitments will be taken into consideration

CONSTRUCTION PHASE

WEEK OF APRIL 7

General Meeting: Captain speech on the leadership policy, Evaluation of Committees by Captain/Faculty Sponsors

Fundamental Research: Continue research Applied Research: 3A Assembly Outreach: Finalize Brochure Web Development: Continue work on the wiki

Captain seeks new recruits for next year's iGEM Team; reveals ultimate vision of the Project to the Upper School; sends the Biotechnology Initiative brochure to underclassmen

WEEK OF APRIL 14

General Meeting: Committee Meetings

Fundamental Research: Finalize research on genes for the detection of VOC biomarkers Applied Research: Work with the Fundamental Research Team on finding the DNA sequences, reinforce laboratory skills, calculate potential budget for future years Outreach: Posters around campus, print out 200 copies of the brochure and distribute to interested science instructors, begin development of a more formal packet of info for the administration Web Development: Assist Outreach, finalize at least half of the wiki

CANCELED UNTIL CONSTRUCT DEVELOPMENT IS COMPLETE: Plan for a UTSW visit April 18

WEEK OF APRIL 21

General Meeting: Brief presentation by Web Development on Thursday

Fundamental Research: Begin creating construct in Gene Designer if necessary, look for options for the purchase of these sequences from biotech companies, check iGEM Registry for existing BioBricks Applied Research: Finalize budget, continue refining laboratory skills, assist the Fundamental Research Team Outreach: Finalize packet for the admininstration, print 10 color copies Web Development: Finalize wiki with detailed content & designs, brief presentation to the Team on how to add/modify content

WEEK OF APRIL 28

General Meeting: Presentation on established construct by the Fundamental Research Team

Fundamental Research: Order sequences, collaborate with the Applied Research Team to address possible issues during the actual creation of the construct Applied Research: Collaborate with the Fundamental Research Team to identify room for error Outreach: Design iGEM wearable gear (t-shirt, sweatshirt, and/or hat) and list cost in a document Web Development: Address iGEM requirements, including Human Practices

WEEK OF MAY 5: AP EXAMINATIONS

General Meeting:

Fundamental Research: Applied Research: Outreach: Web Development:

WEEK OF MAY 12: AP EXAMINATIONS

General Meeting:

Fundamental Research: Applied Research: Outreach: Web Development:

WEEK OF MAY 19

General Meeting:

Fundamental Research: Applied Research: Outreach: Web Development:

WEEK OF MAY 26

General Meeting: Evaluate progress

MAY 30: REGULAR REGISTRATION FOR HIGH SCHOOL JAMBOREE CLOSES, JAMOREE ATTENDANCE FEES DUE

WEEK OF JUNE 2

General Meeting: Evaluate progress

JUNE 8: TRACK SELECTION DUE, PROJECT ABSTRACTS DUE, TEAM ROSTERS DUE

WEEK OF JUNE 9

General Meeting: Evaluate progress


WEEK OF JUNE 16


June 20: Wiki Freeze at 11:59PM Boston time (see: wiki requirements and safety questions)

WEEK OF JUNE 23

Practice presentation, polish poster, submit part to iGEM


JUNE 28: JAMBOREE!

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