Team:SMTexas/Notebook

From 2014hs.igem.org

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<h2><b>Notebook Table of Contents</b></h2><br>
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<table cellspacing="10"><tr><td><a href="#LP" class="buttons width">Leadership Policy</a>
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<a href="#Letter" class="buttons width">Letter to Admin</a></td><td style="padding:0 15px 0 15px;">
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<a href="#Aug-Dec" class="buttons width">August-December</a></td></tr><tr><td>
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<a href="#March" class="buttons width">March</a></td><td style="padding:0 15px 0 15px;">
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<a href="#April" class="buttons width">April</a></td><td style="padding:0 15px 0 15px;">
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<a href="#May" class="buttons width">May</a></td><td style="padding:0 15px 0 15px;">
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<a href="#June" class="buttons width">June</a></td></tr></table>
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<header><h2>Our Notebook</h2></header>
 
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<b><table style="border: medium hidden" color="black"><tr><td><h2>Table of Contents<br><a href="#Aug-Dec">August-December</a><br><a href="#March" >March</a><br><a href="#April" >April</a><br><a href="#May">May</a><br><a href="#June">June</a></h2></td></tr></table></b>
 
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<a id="Aug-Dec"><b>AUGUST-DECEMBER</b></a>
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<header><h2><a id="LP"><b>Leadership Policy</b></a></h2></header></div></section>
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<header><h2><a id="Budget"><b>The Budget</b></a></h2></header></div></section>
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<header><h2><a id="Letter"><b>Letter to Administration</b></a></h2></header></div></section>
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<iframe src="http://docs.google.com/viewer?url=https://static.igem.org/mediawiki/2014hs/2/2f/Letter_to_Ferrell.pdf&embedded=true" width="1200" height="780" style="border: none;"></iframe><br><br>
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<br><a id="Aug-Dec"><b>AUGUST-DECEMBER</b></a>
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The founding of the St. Mark's School of Texas iGEM Team required overcoming many administrative and logistical hurdles from funding to lab space. Through garnering the support of the science department, we were fortunate enough to have a laboratory set aside for us
The founding of the St. Mark's School of Texas iGEM Team required overcoming many administrative and logistical hurdles from funding to lab space. Through garnering the support of the science department, we were fortunate enough to have a laboratory set aside for us
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Outreach: brainstorm ideas about getting more Marksmen interested in iGEM, plan to make a brochure, determine a date to display the posters Killian created around the campus
Outreach: brainstorm ideas about getting more Marksmen interested in iGEM, plan to make a brochure, determine a date to display the posters Killian created around the campus
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Web Development: go over wiki design/layout, ask Mr. Rummel if you all are having trouble
Web Development: go over wiki design/layout, ask Mr. Rummel if you all are having trouble
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October 2, 2013: Our first meeting will be on October 17th during the 10:30 period. We'll be introducing iGEM, an exciting synthetic biology and genetic engineering competition.
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October 8, 2013: Stanford and Brown astrobiology project was presented to give some perspective on what we could do.
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October 16, 2013: Hey guys, I'd just like to remind you all that our first meeting is tomorrow during the 10:30 period. Unless we have an assembly, we will be talking about iGEM and possible projects we'll be doing.
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October 18, 2013: Here are the assignments. You can find team wikis at https://igem.org/Team_Wikis?year=2013&division=high_school. Remember that for now, our meetings will be every Thursday during the 10:30 period in Mr. Adame's room.
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For our next meeting, please come with information about your team's project. Be able to explain what, why, and how. This will help immensely when we start brainstorming ideas for our own iGEM project.
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October 24, 2013: We had a very productive meeting today. Thanks for spending time thoroughly researching your assigned team. You all knew details of your projects very well! For our next meeting, continue looking into your assigned team and focus primarily on cost and parts. See what BioBricks or other resources they utilized to create their project. This next meeting will be the last one focused solely on what other teams have done in the past. We need to start generating ideas for our own project. You can see how one collegiate team that worked on converting CO into CO2 biologically came up with their idea here: https://2012.igem.org/Team:METU/Brainstorm
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November 2, 2013: Hey guys,
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At our next meeting, we'll start brainstorming iGEM projects to pursue. I've seen some great ideas from the leadership applications we've received. Please continue exploring topics we could look into. Science magazines, journals, and blogs are great sources for inspiration. Even the news is a viable source.
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The World iGEM Collegiate Championship Jamboree is also live right now!! You can check out the live videos or the archives of specific teams. Listening to the presentations should give us some valuable insight.
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November 6, 2013: Hey all,
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We have a meeting tomorrow at 10:30. Please bring some iGEM project ideas and if you'd like to apply for a leadership position, remember that the deadline is next Friday, November 15. If you have any questions, please let me know.
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November 7, 2013: I think Gopal mentioned producing bacteria to create O2 to promote healing in bandages. Well Thomas Jefferson High School studied a similar project last year, check it out here: https://2013hs.igem.org/Team:Jefferson_VA_SciCOS/Project. Notice how they took a simple idea and expanded upon it.
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Here are some of the ideas we generated today. Mr. Adame also sent us a link to a great article on treating contaminated water. Check it out below! Remember, we don't have to come up with a completely original idea for our project. We can take a project someone else has worked on and modify it.
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November 10, 2013: Hope you all had a great weekend! Go Lions! I'd just like to update you guys on the direction of our iGEM project. Aarohan, Jeffrey, Nikhil, and I thoroughly considered all of the great ideas you all proposed. This afternoon, we made a final decision; we are pursuing a project centered on probiotic production. After consulting with Dr. Sandeep Burma, we found that creating bacteria that would facilitate enzyme production and promote healing in certain populations would not only have useful applications, but also be practical for us accomplish with the resources we have available.
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November 12, 2013; Hey guys,
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We'll be going over gene expression/regulation during Thursday's meeting. To help lay the groundwork, I have sent you all two PDFs from chapters of the AP Biology textbook on the basics of DNA, transcription, and translation (check your e-mail). I hope you all will scan them over. The two chapters go in depth on what you learned in first year biology. If you're thinking about competing in the USA Biology Olympiad in the spring, Campbell's Biology (the text used in AP Biology) is the official textbook and most questions come from material covered in the book.
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November 17, 2013; Hey guys,
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Check out this TED talk if you have a chance.
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Here's a little intro from TED.com: "Once it's created, plastic (almost) never dies. While in 12th grade Miranda Wang and Jeanny Yao went in search of a new bacteria to biodegrade plastic -- specifically by breaking down phthalates, a harmful plasticizer. They found an answer surprisingly close to home."
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November 20, 2013: If you're interested in iGEM, find some time to go over Unit 1: Biotechnology Techniques on http://www.nature.com/scitable/ebooks/intro-to-biotechnology-techniques-and-applications-16570330/contents. You must know these basic techniques before you can do anything biotechnology related.
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The tutorial covers restriction enzymes, PCR, and the cloning of expressed genes. If you have any questions, feel free to message me this Thanksgiving break.
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December 4, 2013: Hey guys,
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Just a reminder that we are having a regular club meeting tomorrow.
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We have read through all your applications and have determined the remaining leadership positions for this year's Biology Club and iGEM team. We'll announce those and talk about the direction of our iGEM project.
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December 11, 2013: Hey guys,
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We have an important meeting tomorrow. Financial support for our iGEM project is contingent upon your attendance at the next two meetings. But sadly, seniors have a conflict with the meeting time again. Fortunately, Raymond Guo has agreed to lead this meeting. Please try to get to the next two meetings promptly at 10:30.
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December 18, 2013: Hey guys,
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We have a really important meeting tomorrow. Please be there promptly at 10:30. Be sure to give a warm welcome to our three incredible guests tomorrow.
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December 19, 2013: The help and support we have received for our biotechnology initiative is truly incredible. Now with the school's financial backing, we will be able to pursue new frontiers of research and discovery through the iGEM competition.
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To finalize the details of our project, I've created a new Dropbox folder specifically for the iGEM Team. We will start reviewing literature on probiotics over winter break. If you're on the iGEM team, please be on the lookout for documents placed in the iGEM Dropbox folder along with e-mails regarding our project and your individual roles.
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So far, I have contracts of commitment from the following guys. You will receive an invitation to join the folder from the e-mail address below. If you'd like me to send the invitation to an alternate e-mail, please let me know.
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December 21, 2013: I'm really excited to announce that we are now an official iGEM team. You can see our team page and our fellow competitors from around the world at https://igem.org/Team_List?year=2014&division=high_school. Our team name is SMTexas.
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<b>DEVELOPMENTAL PHASE</b>
<b>DEVELOPMENTAL PHASE</b>
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Web Development: go over wiki design/layout, ask Mr. Rummel if you all are having trouble
Web Development: go over wiki design/layout, ask Mr. Rummel if you all are having trouble
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March 19, 2014: Official unveiling of our project direction tomorrow after school. Committee sign-ups will begin as well. I'd like to encourage you all to recruit your friends and peers. We need all the guys we can get, and this is the perfect time for them to join.
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March 22, 2014: Detecting cancer will be challenging, and we need all the help we can get. Please peruse the information in the packet I handed out last Thursday.
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Committee sign ups have begun. If you weren't able to make Thursday's meeting, please fill out this online form. https://docs.google.com/forms/d/19b9k1h3Tb8ORI5F0efW-5QO1xOht0JdmT9-zugtR7lU/viewform. Every member of the iGEM Team must be on a Committee.
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You can find the schedule for the next few weeks at bit.ly/smbiology. As a Committee member, you will be responsible for completing the weekly goals.
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I will send out e-mails regarding the leadership team and the responsibility of your chosen Committee in the next few days as you all sign up. Hope you guys have a nice weekend.
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Web Development: follow iGEM Wiki guidelines and create relevant content, make sure to work on the team page
Web Development: follow iGEM Wiki guidelines and create relevant content, make sure to work on the team page
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March 24, 2014: Meetings Wednesdays and Thursdays after school this week. If you haven't done so already, please sign up for a Committee. Not doing so would infringe the Contract of Commitment you signed in the fall last year.
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iGEM Committee Online Sign Up Form: https://docs.google.com/forms/d/19b9k1h3Tb8ORI5F0efW-5QO1xOht0JdmT9-zugtR7lU/viewform
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If you weren't able to make the meeting last week, be sure to peruse the packet I attached in the e-mail I sent you all. It covers the basics of synthetic biology. Try to understand the information and ask any questions you may have on Wednesday.
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March 27, 2014: Looking at our competition, we began to get behind some of the highly competitive teams, which mostly have University support. Worked extra hard.
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To this end, if you all have a chance, please download these two programs and watch the following tutorial video.
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<b>WEEK OF MARCH 31 </b>
<b>WEEK OF MARCH 31 </b>
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General Meeting: presentation by the Fundamental Research Group on their findings & in-development construct
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General Meeting: Presentation by the Fundamental Research Group on their findings & in-development construct
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Fundamental Research: work with the Applied Research Group to start constructing the plasmid, continue research
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Fundamental Research: Worked with the Applied Research Group to start constructing the plasmid, continue research
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Applied Research: find protocols for implementing the construct, identify problems and consult the Fundamental Research Group
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Applied Research: Found protocols for implementing the construct, identify problems and consult the Fundamental Research Group
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Outreach: brainstorm ideas on promoting the biotechnology further, esp. ideas for getting additional funding, possibly think about hosting parents for a luncheon, e-mail Dr. Burma and any other professors at UTSW for lab tour/advice/guidance
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Outreach: Brainstormed ideas on promoting the biotechnology further, esp. ideas for getting additional funding, possibly think about hosting parents for a luncheon, e-mail Dr. Burma and any other professors at UTSW for lab tour/advice/guidance
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Web Development: document progress and work with the Outreach Group  
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Web Development: Documented progress and work with the Outreach Group  
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POSTPONED B/C of ISAS Mandatory Meeting: APRIL 2 Evaluation: Meeting with Mr. Adame, Mr. Rummel, and Committee Leaders <br><br>
POSTPONED B/C of ISAS Mandatory Meeting: APRIL 2 Evaluation: Meeting with Mr. Adame, Mr. Rummel, and Committee Leaders <br><br>
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ISAS Commitments will be taken into consideration
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ISAS Commitments are taken into consideration
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General Meeting: Captain speech on the leadership policy, Evaluation of Committees by Captain/Faculty Sponsors
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General Meeting: Captain spoke on the leadership policy, Evaluation of Committees by Captain/Faculty Sponsors
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Fundamental Research: Continue research
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Fundamental Research: Continue research;  Finalized research on genes for the detection of VOC biomarkers
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Applied Research: 3A Assembly
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Applied Research: 3A Assembly; Worked with the Fundamental Research Team on finding the DNA sequences, reinforced laboratory skills, calculated potential budget for future years
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Outreach: Finalize Brochure
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Outreach: Finalized Brochure; Placed Posters around campus, printed out 200 copies of the brochure and distributed to interested science instructors, began development of a more formal packet of info for the administration
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Web Development: Continue work on the wiki
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Web Development: Continued work on the wiki
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April 7, 2014: Made the goals for the week. Held Regular meetings Wednesday and Thursday. <br><br>
Captain seeks new recruits for next year's iGEM Team; reveals ultimate vision of the Project to the Upper School; sends the Biotechnology Initiative brochure to underclassmen
Captain seeks new recruits for next year's iGEM Team; reveals ultimate vision of the Project to the Upper School; sends the Biotechnology Initiative brochure to underclassmen
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Fundamental Research: Finalize research on genes for the detection of VOC biomarkers
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Fundamental Research: Finalized research on genes for the detection of VOC biomarkers
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Applied Research: Work with the Fundamental Research Team on finding the DNA sequences, reinforce laboratory skills, calculate potential budget for future years
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Applied Research: Worked with the Fundamental Research Team on finding the DNA sequences, reinforced laboratory skills, calculated potential budget for future years
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Outreach: Posters around campus, print out 200 copies of the brochure and distribute to interested science instructors, begin development of a more formal packet of info for the administration
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Outreach: Placed posters around campus, print out 200 copies of the brochure and distributed to interested science instructors, began development of a more formal packet of info for the administration
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Web Development: Assist Outreach, finalize at least half of the wiki
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Web Development: Assisted Outreach, finalized at least half of the wiki
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Fundamental Research: Begin creating construct in Gene Designer if necessary, look for options for the purchase of these sequences from biotech companies, check iGEM Registry for existing BioBricks
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Fundamental Research: Began creating construct in Gene Designer if necessary; looked for options for the purchase of these sequences from biotech companies, check iGEM Registry for existing BioBricks
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Applied Research: Finalize budget, continue refining laboratory skills, assist the Fundamental Research Team
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Applied Research: Finalized budget, continue refining laboratory skills, assist the Fundamental Research Team
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Outreach: Finalize packet for the admininstration, print 10 color copies
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Outreach: Finalized packet for the admininstration, print 10 color copies
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Web Development: Finalize wiki with detailed content & designs, brief presentation to the Team on how to add/modify content
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Web Development: Finalized wiki with detailed content & designs, brief presentation to the Team on how to add/modify content
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Fundamental Research: N/A
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Outreach: N/A
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Web Development: N/A
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General Meeting:
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General Meeting: Meeting Cancelled
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Fundamental Research:
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Fundamental Research: N/A
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Outreach: N/A
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Web Development: N/A
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May 16, 2014: We're planning to order iGEM Shirts regardless of whether we attend the Jamboree. If you would like one, please fill out this quick online form.
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<b>WEEK OF MAY 19</b>
<b>WEEK OF MAY 19</b>
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Fundamental Research:
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Fundamental Research: Prepared to finalize gene constructs; Looked for any last minute corrections and do any other research that will aid in later implementation.
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Applied Research:
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Applied Research: Continued working with 3A assembly; Worked with basic research team and prepare to implement concepts and ideas of basic research team.
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Outreach:
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Outreach: Continued working on human practices, including community outreach and global outreach; Continued filming as well
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Web Development:
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Web Development: Worked extensively on the wiki; write more content; Worked with other committees to do so
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Fundamental Research:  
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Fundamental Research: Continued piecing together constructs and preparing for implementation
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Applied Research: Prepare lab space and order necessary materials and do orientation for summer wet lab work and research.
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Applied Research: Prepared lab space and order necessary materials and do orientation for summer wet lab work and research
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Outreach:  
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Outreach: Continued human practices as mentioned above; Worked with other committees as well
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Web Development: Worked extensively on the wiki and worked with other committees
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Fundamental Research: Researched novel solutions to the genetic construct, found outstanding research paper, continued research on potential VOC biomarkers; Arrived after lunch, researched solutions to mechanism discrepancies, Aste gene; gene sequence, researched solutions to constructing genetic circuits, Applied--developed prototype genetic circuit system, assigned special duties, specified equipment available, purchased additional equipment and poster;
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Applied Research: Do another 3A Assembly and analyze possibility of Gibson Assembly. Work with Fundamental Research to help identify genes.
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Applied Research: Did another 3A Assembly and analyze possibility of Gibson Assembly. Worked with Fundamental Research to help identify genes. Made bacteria cultures, prepared more ampicillin and kanamycin solutions, found gene sequence for RFP; applied research: prepared more ampicillin and kanamycin bacteria cultures, miniprep; Did gel electrophoresis previous day's miniprep; all were successful. Worked with Basic Research to map and understand a potetnial mechanism for the expression of the aldb gene; Arrived after lunch, helped research potential genetic circuit solutions; developed prototype genetic circuit system, assigned special duties, specified equipment available, purchased additional equipment and poster
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Outreach:  
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Outreach: Created a detailed production timeline for the first week, brainstormed and came up with a mind map for the film, help decide the wiki design layout;
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Web Development: Worked extensively on the wiki, added interactive elements and modified existing layout significantly
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Fundamental Research:  
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Fundamental Research: Helped applied research as well as other committees that require help
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Applied Research: Finish and analyze 3A Assembly and finish work with Fundamental Research Committee. Also work with Outreach Committee on Poster.
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Applied Research: Finished and analyze 3A Assembly and finish work with Fundamental Research Committee; Also worked with Outreach Committee on Poster
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Outreach:  
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Outreach: Continued work with human practices
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Web Development: Continued work on wiki and work with other committees
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General Meeting: Evaluate progress
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General Meeting: Evaluated progress
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Fundamental Research:  
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Fundamental Research: Helped other committees
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Applied Research: Work with Outreach Team.
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Applied Research: Worked with Outreach Team.
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Outreach:  
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Outreach: Worked on human practices (extensively)
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Web Development: Worked on wiki (extensively)
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Latest revision as of 04:00, 21 June 2014