Team:SMTexas/Implementation

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<header><h2>The Implementation</h2></header>
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The VOC biosensor for lung cancer will be implemented to detect lung cancer in its early stages through noninvasive means in an effective and low-cost manner. When the VOC comes in contact with the bacterial plate, it will cause the transcription of the gene and the translation of the protein that will cause the bacteria to glow. In contrast to current lung cancer detection mechanisms, this biosensor will be able to "smell" the cancer and indicate its presence in a way that is both more effective and less expensive than current techniques. This new device can be a great discovery for people who live in poor countries. Countless individuals succumb to this ghastly disease in these nations, and a non-expensive and noninvasive method to detect lung cancer early can save these lives.
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While the development of mechanical and electronic noses have been in progress for many decades, we propose that a circuit of genetically-modified E. coli used in conjunction with a mechanical contraption has the potential to be used a preliminary diagnostic for lung cancer. We propose a device similar to a breathalyzer that utilizes a cylindrical container with our genetically engineered E. coli systems at the bottom of the aluminum shell. To prevent the possibility of contaminants, an adapter would attach to the top of the cylindrical shell for the user to breath into. The user would fasten the adapter onto the shell and exhale as much as possible. Upon exhalation, the user would immediately detach the adapter to secure the system designed to automatically shut off after the removal of the adapter and the shell. The genetic systems would be underlain with a sensitive paper that would be dyed upon the expression of the fluorescent proteins. We would utilize Cambridge 2009 iGEM Team’s E. Chromi color generator to verify and differentiate the identities of the VOCs present in the user’s breath sample. Currently under development by our team, this device has the potential to be an inexpensive, sensitive, and noninvasive solution to early screening of lung cancer.
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Latest revision as of 03:53, 21 June 2014