Team:SMTexas/Design

From 2014hs.igem.org

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<h5>frmR Gene (Detects Formaldehyde) </h5>
<h5>frmR Gene (Detects Formaldehyde) </h5>
Formaldehyde induces the frmR gene, functioning as a regulatory gene of Green Fluorescent protein. When transcribed, it expresses a regulatory protein that binds to an downstream operator that prevents the movement of RNA polymerase. Under typical conditions, the promoter downstream of the regulatory gene increases the affinity of RNA polymerase to the DNA strand, but the transcription enzyme cannot bypass the operator and transcribe GFP, the gene that is ultimately under regulation. In such a scenario, the regulatory protein that frmR expresses functions as a repressor and effectively inhibits transcription of the coding sequence. Formaldehyde, on the other hand, induces the transcription of GFP and ultimately causes bacterial fluorescence. Acting as a corepressor, the VOC binds to the regulatory protein and conforms it into an inactive shape, allowing for the passage of RNA polymerase through the operator and transcribe the GFP protein.
Formaldehyde induces the frmR gene, functioning as a regulatory gene of Green Fluorescent protein. When transcribed, it expresses a regulatory protein that binds to an downstream operator that prevents the movement of RNA polymerase. Under typical conditions, the promoter downstream of the regulatory gene increases the affinity of RNA polymerase to the DNA strand, but the transcription enzyme cannot bypass the operator and transcribe GFP, the gene that is ultimately under regulation. In such a scenario, the regulatory protein that frmR expresses functions as a repressor and effectively inhibits transcription of the coding sequence. Formaldehyde, on the other hand, induces the transcription of GFP and ultimately causes bacterial fluorescence. Acting as a corepressor, the VOC binds to the regulatory protein and conforms it into an inactive shape, allowing for the passage of RNA polymerase through the operator and transcribe the GFP protein.
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<table><tr><td width="1200" align="center"><img src="https://static.igem.org/mediawiki/2014hs/1/11/FrmR.png"></td></tr></table>
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<table><tr><td width="1200" align="left"><img src="https://static.igem.org/mediawiki/2014hs/1/11/FrmR.png"></td><td width="1200" align="right"><img src="https://static.igem.org/mediawiki/2014hs/3/3e/FrmR2.png"></td></tr></table>
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Revision as of 01:22, 20 June 2014