Team:SMTexas/Design

From 2014hs.igem.org

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<h4> XylR Gene (Detects Xylene) </h4>
<h4> XylR Gene (Detects Xylene) </h4>
The genetically-related expression of the XylR gene consists of promoters, regulator complexes, and proteins that all aid in the expression of fluorescent proteins. The initial DNA sequence Pr promotes the expression of XylR gene exon itself. Shortly after, it is followed by a ribosomal binding site that orchestrates the timing and efficiency of translation. The naturally expressing XylR sequence succeeds the RBS and undergoes strict regulation of the Pr promoter. Because this protein triggers a secondary response in the bacterium which is vital to Xylene detection, a double termination sequence is essential to the discontinuation of sequences downstream of the XylR coding region is not expressed which can disrupt the reactions involved in the detection system. These stop codons, which are short and effective, operate with a stem-loop that possesses both forward and reverse termination mechanisms. The expressed XylR protein then reacts with xylene and is conformed to accommodate a secondary gene sequence. This newly conformed version of the protein can then bind to the Pu promoter. After a second ribosomal binding site (strong) is subsequently intiated and YFP is expressed, which supersedes the RBS, the bacteria will exhibit yellow fluorescence to indicate a positive test.
The genetically-related expression of the XylR gene consists of promoters, regulator complexes, and proteins that all aid in the expression of fluorescent proteins. The initial DNA sequence Pr promotes the expression of XylR gene exon itself. Shortly after, it is followed by a ribosomal binding site that orchestrates the timing and efficiency of translation. The naturally expressing XylR sequence succeeds the RBS and undergoes strict regulation of the Pr promoter. Because this protein triggers a secondary response in the bacterium which is vital to Xylene detection, a double termination sequence is essential to the discontinuation of sequences downstream of the XylR coding region is not expressed which can disrupt the reactions involved in the detection system. These stop codons, which are short and effective, operate with a stem-loop that possesses both forward and reverse termination mechanisms. The expressed XylR protein then reacts with xylene and is conformed to accommodate a secondary gene sequence. This newly conformed version of the protein can then bind to the Pu promoter. After a second ribosomal binding site (strong) is subsequently intiated and YFP is expressed, which supersedes the RBS, the bacteria will exhibit yellow fluorescence to indicate a positive test.
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<table><tr><td width="1200" align="left"><img src="https://static.igem.org/mediawiki/2014hs/a/a5/XylR_Map.png"></td></tr></table>
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<table><tr><td width="1200" align="left"><img src="https://static.igem.org/mediawiki/2014hs/a/a5/XylR_Map.png"></td><td width="1200" align="right"><img src="https://static.igem.org/mediawiki/2014hs/6/6a/XylR2.png"></td></tr></table>
   
   
<h5>frmR Gene (Detects Formaldehyde) </h5>
<h5>frmR Gene (Detects Formaldehyde) </h5>

Revision as of 01:20, 20 June 2014