Team:SMTexas/Design

From 2014hs.igem.org

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<h3> aldB Gene (Detects Ethanol) </h3>
<h3> aldB Gene (Detects Ethanol) </h3>
The aldB gene codes for a functional aldehyde dehydrogenase, which is directly induced by ethanol. Metabolism of the alcohol consists of its transformation into an aldehyde and then into a carboxylic acid in activating several related pathways. The acid reacts with the BarA histidine sensory kinase, a signalling enzyme involved in a two component signal transduction system present in E. coli, to catalyze the breakdown of various carboxylic acids. The kinase is additionally responsible for the induction of RpoS, a regulatory gene of aldB that directly opposes fis during the activation of the aldB operon. Eventually, BarA triggers a series of vital reactions that affect the Crp-cAMP regulatory mechanism, a dual complex that controls the expression of the aldB coding sequence. In the complex, cAMP conforms the shape of Crp, also known as CAP (catabolite activator protein). This newly conformed Crp then attaches to the promoter and contributes to the initiation of transcription of the aldB operon. Downstream of aldB, CFP (cyan fluorescent protein) is expressed and the bacteria exhibits cyan fluorescence.<br>
The aldB gene codes for a functional aldehyde dehydrogenase, which is directly induced by ethanol. Metabolism of the alcohol consists of its transformation into an aldehyde and then into a carboxylic acid in activating several related pathways. The acid reacts with the BarA histidine sensory kinase, a signalling enzyme involved in a two component signal transduction system present in E. coli, to catalyze the breakdown of various carboxylic acids. The kinase is additionally responsible for the induction of RpoS, a regulatory gene of aldB that directly opposes fis during the activation of the aldB operon. Eventually, BarA triggers a series of vital reactions that affect the Crp-cAMP regulatory mechanism, a dual complex that controls the expression of the aldB coding sequence. In the complex, cAMP conforms the shape of Crp, also known as CAP (catabolite activator protein). This newly conformed Crp then attaches to the promoter and contributes to the initiation of transcription of the aldB operon. Downstream of aldB, CFP (cyan fluorescent protein) is expressed and the bacteria exhibits cyan fluorescence.<br>
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<table><tr><td width="1200" align="center"><img src="https://static.igem.org/mediawiki/2014hs/7/74/AldB_Map.png"></td></tr></table>  
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<table><tr><td width="1200" align="left"><img src="https://static.igem.org/mediawiki/2014hs/7/74/AldB_Map.png"></td><td align="right"><img src="https://static.igem.org/mediawiki/2014hs/0/00/AldB2.png"></td></tr></table>  
<h4> XylR Gene (Detects Xylene) </h4>
<h4> XylR Gene (Detects Xylene) </h4>

Revision as of 01:17, 20 June 2014