Team:SMTexas/Design

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<h5>frmR Gene (Detects Formaldehyde) </h5>
<h5>frmR Gene (Detects Formaldehyde) </h5>
The frmR gene is induced by formaldehyde, a VOC that exists in higher concentrations in the breath of lung cancer patients.  In E. Coli, frmR is followed by a promoter, which in turn is followed by an operator and the GFP coding gene. frmR, which functions as a regulatory gene, codes for a protein which acts as an active repressor for the operator.  Thus, RNA polymerase cannot pass through the operator and transcribe GFP. Formaldehyde, which acts as a co-repressor, would bind to the protein and conform it into an inactive shape, allowing the RNA polymerase to pass through the operator and transcribe the GFP protein.  Thus, if formaldehyde is present, the GFP gene is expressed, and the bacteria will glow in the presence of the VOC.  
The frmR gene is induced by formaldehyde, a VOC that exists in higher concentrations in the breath of lung cancer patients.  In E. Coli, frmR is followed by a promoter, which in turn is followed by an operator and the GFP coding gene. frmR, which functions as a regulatory gene, codes for a protein which acts as an active repressor for the operator.  Thus, RNA polymerase cannot pass through the operator and transcribe GFP. Formaldehyde, which acts as a co-repressor, would bind to the protein and conform it into an inactive shape, allowing the RNA polymerase to pass through the operator and transcribe the GFP protein.  Thus, if formaldehyde is present, the GFP gene is expressed, and the bacteria will glow in the presence of the VOC.  
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<table><tr><td width="1200" align="center"><img src="https://static.igem.org/mediawiki/2014hs/a/a5/XylR_Map.png"></td></tr></table>
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<table><tr><td width="1200" align="center"><img src="https://static.igem.org/mediawiki/2014hs/1/11/FrmR.png"></td></tr></table>
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Revision as of 15:41, 11 June 2014