Team:SMTexas

From 2014hs.igem.org

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This is a template page. READ THESE INSTRUCTIONS.
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have the following information on your wiki:
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body {
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<ul style="font-weight:normal;">
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font: 100%/1.4 Verdana, Arial, Helvetica, sans-serif;
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<li>a team description</li>
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background-color: #42413C;
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<li>project description</li>
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margin: 0;
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<li>safety information (did your team take a safety training course? were you supervised in the lab?)</li>
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padding: 0;
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<li>team attribution (who did what part of your project?)</li>
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<li>lab notebook</li>
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<li>sponsor information</li>
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<li>other information</li>
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REMEMBER, keep all of your pages within your teams namespace. <br><span style="font-weight:normal; font-style:italic;">Example: 2013hs.igem.org/Team:SMTexas/Our_Pets</span>
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1) Padding is only placed on the top and/or bottom of the block elements. The elements within these blocks have padding on their sides. This saves you from any "box model math". Keep in mind, if you add any side padding or border to the block itself, it will be added to the width you define to create the *total* width. You may also choose to remove the padding on the element in the block element and place a second block element within it with no width and the padding necessary for your design.
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2) No margin has been given to the columns since they are all floated. If you must add margin, avoid placing it on the side you're floating toward (for example: a right margin on a block set to float right). Many times, padding can be used instead. For blocks where this rule must be broken, you should add a "display:inline" declaration to the block element's rule to tame a bug where some versions of Internet Explorer double the margin.
 
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3) Since classes can be used multiple times in a document (and an element can also have multiple classes applied), the columns have been assigned class names instead of IDs. For example, two sidebar blocks could be stacked if necessary. These can very easily be changed to IDs if that's your preference, as long as you'll only be using them once per document.
 
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4) If you prefer your nav on the left instead of the right, simply float these columns the opposite direction (all left instead of all right) and they'll render in reverse order. There's no need to move the blocks around in the HTML source.
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{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:SMTexas_logo.png|200px|right|frame]]
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|-
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|
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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|[[Image:SMTexas_team.png|right|frame|Your team picture]]
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|-
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|align="center"|[[Team:SMTexas | Team SMTexas]]
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|}
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*/
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!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=SMTexas Official Team Profile]
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|}
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===Team===
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Tell us about your team, your school!
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list-style: none; /* this removes the list marker */
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We are a nondenominational independent school in Dallas, Texas.
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===Project===
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Lung cancer is the leading cause of cancer-related death around the world. Early detection systems would enable us to diagnose the disease at a time when therapeutic intervention would be most effective. Noninvasive breath sampling has the potential to save lives as approximately 86% of lung cancer patients die within five years of diagnosis. Early detection improves survival rate by 50% from stage III to stage I of the cancer. Breath biochemical sensors also have important applications for other diseases. According to the World Health Organization�s latest report on noncommunicable diseases (NCDs), these �diseases are the leading global causes of death, causing more deaths than all other causes combined.� Further research into these noninvasive tests while maintaining the tests� accuracy and sensitivity could have a major impact on survival rates of other NCDs. Tests of this nature would reduce inequity and provide improved means of treatment for affected individuals regardless of nationality, income, or access to healthcare.
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position: relative;/* this gives IE6 hasLayout to properly clear */
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clear: both; /* this clear property forces the .container to understand where the columns end and contain them */
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}
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/*HTML 5 support - Sets new HTML 5 tags to display:block so browsers know how to render the tags properly. */
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While invasive techniques, such as immunofluorescence and Western Blotting already exist to detect the Epidermal Growth Factor Receptor (EGFR) biomarker, breath chemical tests via genetically modified Escherichia coli bacteria would be a novel and versatile method of lung cancer screening. Twenty-two volatile organic compounds (VOCs) have been found to be unique to the breath of affected patients, creating a viable �fingerprint� for reliable detection (Horvath et al.). We will utilize these concepts to create a biosensor for lung cancer.
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header, section, footer, aside, article, figure {
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Our team will design a system combining existing BioBricks along with novel synthesized biological parts to create a whole-cell bacterial sensor. We will attempt to create a breath biosensor through noninvasive intervention of lung cancer. Implementing recombinant DNA technology, the bacteria will be able to respond to environmental queues through a receptor-dependent pathway. The construct will fuse a DNA response element with reporter genes to create a portable biosensor. We are currently investigating E. coli promoters or alternative sigma factors in relation to our intended target compounds.
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<div class="container">
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===Notebook===
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  <header>
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On Tuesday, February 18th, Vikrant R, Rohin M, and Gopal R, inoculated bacterial strains A & B onto LB agar with ampicillin & kanamycin and incubated overnight at 37C.
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    [[Image:SMTexas_logo.png|200px|left|frame]]
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  </header>
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On Wednesday, February 19th, Vikrant R & Rohin M transferred one colony from each of A & B and inoculated in LB BROTH with ampicillin & kanamycin and placed in shaking water bath at 37C.
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  <div class="sidebar1">
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The next step is to perform a miniprep, which will allow us to extract plasmid from each A & B bacterial strains, which will then allow us do the restriction digest and ligation.  
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    <ul>
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      <li><a href="#">Link one</a></li>
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All of these steps can be seen in the 3A Assembly kit protocols
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      <li><a href="#">Link two</a></li>
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      <li><a href="#">Link three</a></li>
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===Results/Conclusions===
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      <li><a href="#">Link four</a></li>
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What did you achieve over the course of your semester?
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    </ul>
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    <aside>
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===Safety===
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      <p> The above links demonstrate a basic navigational structure using an unordered list styled with CSS. Use this as a starting point and modify the properties to produce your own unique look. If you require flyout menus, create your own using a Spry menu, a menu widget from Adobe's Exchange or a variety of other javascript or CSS solutions.</p>
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What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
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      <p>If you would like the navigation along the top, simply move the ul to the top of the page and recreate the styling.</p>
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===Attributions===
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  <article class="content">
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Who worked on what?
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    <h1>Instructions</h1>
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    <section>
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    <h2>How to use this document</h2>
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===Human Practices===
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      <p>Be aware that the CSS for these layouts is heavily commented. If you do most of your work in Design view, have a peek at the code to get tips on working with the CSS for the fixed layouts. You can remove these comments before you launch your site. To learn more about the techniques used in these CSS Layouts, read this article at Adobe's Developer Center - <a href="http://www.adobe.com/go/adc_css_layouts">http://www.adobe.com/go/adc_css_layouts</a>.</p>
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What impact does/will your project have on the public? 
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===Fun!===
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      <p>Because all the columns are floated, this layout uses a clear:both declaration in the footer rule.  This clearing technique forces the .container to understand where the columns end in order to show any borders or background colors you place on the .container. If your design requires you to remove the footer from the .container, you'll need to use a different clearing method. The most reliable will be to add a &lt;br class=&quot;clearfloat&quot; /&gt; or &lt;div  class=&quot;clearfloat&quot;&gt;&lt;/div&gt; after your final floated column (but before the .container closes). This will have the same clearing effect. </p>
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What was your favorite team snack?? Have a picture of your team mascot?
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      <p>An image placeholder was used in this layout in the header where you'll likely want to place  a logo. It is recommended that you remove the placeholder and replace it with your own linked logo. </p>
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<forum_subtle />
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      <p> Be aware that if you use the Property inspector to navigate to your logo image using the SRC field (instead of removing and replacing the placeholder), you should remove the inline background and display properties. These inline styles are only used to make the logo placeholder show up in browsers for demonstration purposes. </p>
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      <p>To remove the inline styles, make sure your CSS Styles panel is set to Current. Select the image, and in the Properties pane of the CSS Styles panel, right click and delete the display and background properties. (Of course, you can always go directly into the code and delete the inline styles from the image or placeholder there.)</p>
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      <h2>Backgrounds</h2>
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      <p>By nature, the background color on any block element will only show for the length of the content. This means if you're using a background color or border to create the look of a side column, it won't extend all the way to the footer but will stop when the content ends. If the .content block will always contain more content, you can place a border on the .content block to divide it from the column.</p>
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      Address Content
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Revision as of 02:17, 4 April 2014


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have the following information on your wiki:
  • a team description
  • project description
  • safety information (did your team take a safety training course? were you supervised in the lab?)
  • team attribution (who did what part of your project?)
You may also wish to add other page such as:
  • lab notebook
  • sponsor information
  • other information
REMEMBER, keep all of your pages within your teams namespace.
Example: 2013hs.igem.org/Team:SMTexas/Our_Pets



You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
SMTexas logo.png

Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)

File:SMTexas team.png
Your team picture
Team SMTexas


Official Team Profile

Contents

Team

Tell us about your team, your school! We are a nondenominational independent school in Dallas, Texas.

Project

Lung cancer is the leading cause of cancer-related death around the world. Early detection systems would enable us to diagnose the disease at a time when therapeutic intervention would be most effective. Noninvasive breath sampling has the potential to save lives as approximately 86% of lung cancer patients die within five years of diagnosis. Early detection improves survival rate by 50% from stage III to stage I of the cancer. Breath biochemical sensors also have important applications for other diseases. According to the World Health Organization�s latest report on noncommunicable diseases (NCDs), these �diseases are the leading global causes of death, causing more deaths than all other causes combined.� Further research into these noninvasive tests while maintaining the tests� accuracy and sensitivity could have a major impact on survival rates of other NCDs. Tests of this nature would reduce inequity and provide improved means of treatment for affected individuals regardless of nationality, income, or access to healthcare.

While invasive techniques, such as immunofluorescence and Western Blotting already exist to detect the Epidermal Growth Factor Receptor (EGFR) biomarker, breath chemical tests via genetically modified Escherichia coli bacteria would be a novel and versatile method of lung cancer screening. Twenty-two volatile organic compounds (VOCs) have been found to be unique to the breath of affected patients, creating a viable �fingerprint� for reliable detection (Horvath et al.). We will utilize these concepts to create a biosensor for lung cancer.

Our team will design a system combining existing BioBricks along with novel synthesized biological parts to create a whole-cell bacterial sensor. We will attempt to create a breath biosensor through noninvasive intervention of lung cancer. Implementing recombinant DNA technology, the bacteria will be able to respond to environmental queues through a receptor-dependent pathway. The construct will fuse a DNA response element with reporter genes to create a portable biosensor. We are currently investigating E. coli promoters or alternative sigma factors in relation to our intended target compounds.

Notebook

On Tuesday, February 18th, Vikrant R, Rohin M, and Gopal R, inoculated bacterial strains A & B onto LB agar with ampicillin & kanamycin and incubated overnight at 37C.

On Wednesday, February 19th, Vikrant R & Rohin M transferred one colony from each of A & B and inoculated in LB BROTH with ampicillin & kanamycin and placed in shaking water bath at 37C.

The next step is to perform a miniprep, which will allow us to extract plasmid from each A & B bacterial strains, which will then allow us do the restriction digest and ligation.

All of these steps can be seen in the 3A Assembly kit protocols

Results/Conclusions

What did you achieve over the course of your semester?


Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?


Attributions

Who worked on what?


Human Practices

What impact does/will your project have on the public?


Fun!

What was your favorite team snack?? Have a picture of your team mascot?


<forum_subtle />