Team:SMTexas

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{{SMTexas/heading}}
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This is a template page. READ THESE INSTRUCTIONS.
 
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
 
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You <strong>MUST</strong> have the following information on your wiki:
 
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<li>a team description</li>
 
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<li>project description</li>
 
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<li>safety information (did your team take a safety training course? were you supervised in the lab?)</li>
 
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REMEMBER, keep all of your pages within your teams namespace. <br><span style="font-weight:normal; font-style:italic;">Example: 2013hs.igem.org/Team:SMTexas/Our_Pets</span>
 
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<h1><a href="https://2014hs.igem.org/wiki/index.php?title=Team:SMTexas" class="nav-link"><img src="https://static.igem.org/mediawiki/2014hs/6/65/SMiGEM_Logo_%26_Text.png" alt=""></a></h1>
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{|align="justify"
 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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|[[Image:SMTexas_team.png|right|frame|Your team picture]]
 
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|align="center"|[[Team:SMTexas | Team SMTexas]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=SMTexas Official Team Profile]
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===Team===
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Tell us about your team, your school!
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We are a nondenominational independent school in Dallas, Texas.
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===Project===
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Lung cancer is the leading cause of cancer-related death around the world. Early detection systems would enable us to diagnose the disease at a time when therapeutic intervention would be most effective. Noninvasive breath sampling has the potential to save lives as approximately 86% of lung cancer patients die within five years of diagnosis. Early detection improves survival rate by 50% from stage III to stage I of the cancer. Breath biochemical sensors also have important applications for other diseases. According to the World Health Organization’s latest report on noncommunicable diseases (NCDs), these “diseases are the leading global causes of death, causing more deaths than all other causes combined.” Further research into these noninvasive tests while maintaining the tests’ accuracy and sensitivity could have a major impact on survival rates of other NCDs. Tests of this nature would reduce inequity and provide improved means of treatment for affected individuals regardless of nationality, income, or access to healthcare.
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While invasive techniques, such as immunofluorescence and Western Blotting already exist to detect the Epidermal Growth Factor Receptor (EGFR) biomarker, breath chemical tests via genetically modified Escherichia coli bacteria would be a novel and versatile method of lung cancer screening. Twenty-two volatile organic compounds (VOCs) have been found to be unique to the breath of affected patients, creating a viable “fingerprint” for reliable detection (Horvath et al.). We will utilize these concepts to create a biosensor for lung cancer.
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Our team will design a system combining existing BioBricks along with novel synthesized biological parts to create a whole-cell bacterial sensor. We will attempt to create a breath biosensor through noninvasive intervention of lung cancer. Implementing recombinant DNA technology, the bacteria will be able to respond to environmental queues through a receptor-dependent pathway. The construct will fuse a DNA response element with reporter genes to create a portable biosensor. We are currently investigating E. coli promoters or alternative sigma factors in relation to our intended target compounds.
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===Notebook===
 
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On Tuesday, February 18th, Vikrant R, Rohin M, and Gopal R, inoculated bacterial strains A & B onto LB agar with ampicillin & kanamycin and incubated overnight at 37C.
 
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On Wednesday, February 19th, Vikrant R & Rohin M transferred one colony from each of A & B and inoculated in LB BROTH with ampicillin & kanamycin and placed in shaking water bath at 37C.
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<h2>Fundamental Research</h2>
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<p class="subtitle">Explore how our Fundamental Research Team fueled our project development and research.<br><br></p>
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<img src="https://static.igem.org/mediawiki/2014hs/5/5e/Basic_Research_PNG.png" alt="">
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The next step is to perform a miniprep, which will allow us to extract plasmid from each A & B bacterial strains, which will then allow us do the restriction digest and ligation.  
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<h2>Applied Research<br><br></h2>
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<p class="subtitle">Explore how we have made biobricks that can detect lung cancer in a simple, inexpensive, and noninvasive way.</p>
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<img src="https://static.igem.org/mediawiki/2014hs/8/83/Applied_Research_PNG.png" alt="">
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<h2>Outreach<br><br></h2>
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<p class="subtitle">Educating our community and gathering public opinion on modern biotechnology.<br><br></p>
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<img src="https://static.igem.org/mediawiki/2014hs/2/2d/Outreach_PNG.png" alt="">
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<h2>Videos<br><br></h2>
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<p class="subtitle">Watch the video explaining our project in detail as well as numerous informative tutorials.<br><br></p>
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<img src="https://static.igem.org/mediawiki/2014hs/d/de/Video_PNG.png" alt="">
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<a href="https://2014hs.igem.org/wiki/index.php?title=Team:SMTexas/Video&action=edit&redlink=1" class="button">More</a>
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<h2>Safety</h2>
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<p class="subtitle">Our safety precautions.<br><br><br></p>
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<p><img src="https://static.igem.org/mediawiki/2014hs/f/f9/Safety_PNG.png" alt=""></p>
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All of these steps can be seen in the 3A Assembly kit protocols
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<h2>Attributions</h2>
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<p class="subtitle">Meet the faculty members who played an instrumental role to our success.</p>
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<p><img src="https://static.igem.org/mediawiki/2014hs/6/64/Attributions_PNG.png" alt=""></p>
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<h2>Human Practices</h2>
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<p class="subtitle">Our project in the real world.</p><br><br>
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<p><img src="https://static.igem.org/mediawiki/2014hs/5/5d/Human_Practices_PNG.png"></p>
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<h2>Sponsors</h2>
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<p class="subtitle">Meet the corporations that played an instrumental role in our success.</p><br>
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<p><img src="https://static.igem.org/mediawiki/2014hs/9/90/Sponsors_PNG.png" alt=""></p>
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<a href="https://2014hs.igem.org/Team:SMTexas/Sponsors" class="button">More</a>
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<h2>Biotechnology Initiative</h2>
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<p>The first iGEM high school team from the state of Texas, the St. Mark’s iGEM Team was established as part of a Biotechnology Initiative begun in September of 2013 by our school’s Biology Club. This Initiative, spurred on by iGEM, revitalized our Club and helped generate significant interest in synthetic biology from the student body, faculty, and school administration. In our first year we achieved regular access to a radiation oncology lab at the University of Texas Southwestern Medical Center, $1500 in funding from the school to cover the registration fee for the iGEM Competition, exlcusive laboratory space at the school, and a more than four-fold increase in membership of the Biology Club to twenty-two members.
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Our iGEM Team blends the setup of a traditional club with the highly efficient structure of a professional research laboratory. The Team is divided into four Committees: Fundamental Research, Applied Research, Outreach, and Web Development. This allows us to work more efficiently and cohesively. We have had to lay the foundation of our iGEM Team this year by informing the school community on synthetic biology, seeking financial support, and establishing laboratory resources for our project. But it was our infectious enthusiasm and indefatigable determination for synthetic biology that allowed us to overcome administrative hurdles and a steep learning curve. Through our journey this year, we have proven that a school with limited resources in biotechnology can make significant strides, effectively pioneer investigations in the field, and shown our community the tremendous potential we have in the future.</p>
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<h2>About Us</h2>
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<p><b>Our Mission Statement:</b> To improve the community by pioneering investigations into bio-technology by solving real-world problems in order to promote scientific inquiry and educate the future leaders of biological engineering.</p>
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<p><b>About St. Mark's:</b> St. Mark's School of Texas is a non-sectarian, college-preparatory, independent day school for boys in grades one through twelve. The School's charter states that it is "designed to afford its students well-rounded physical, intellectual, moral, and religious training and instruction." The School is intended to be a diverse community of teachers and students who share a love of learning and who strive for high achievement in whatever they undertake.</p>
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<table><tr><td width="300" align="center"><a href="http://www.smtexas.org/home" class="button">Visit Our School</a></td><td width="300 align="center"><a href="https://2014hs.igem.org/Team:SMTexas/team" class="button">The Team</a></td></tr></table>
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<h2><br><br>Follow us on:</h2>
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<a href="http://www.facebook.com/smbiology"><img src="https://static.igem.org/mediawiki/2014hs/f/fd/Facebook.jpg" alt=""></a>
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<p>Facebook</p>
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<a href="http://www.twitter.com/smbiology"><img src="https://static.igem.org/mediawiki/2014hs/2/2a/Twitter.jpg" alt=""></a>
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<p>Twitter</p>
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<a href="http://instagram.com/smbiology"><img src="https://static.igem.org/mediawiki/2014hs/f/f7/Instagram.jpg" alt=""></a>
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<p>Instagram</p>
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<a href="http://youtube.com/smbiology"><img src="https://static.igem.org/mediawiki/2014hs/5/56/Youtube.jpg" alt=""></a>
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===Results/Conclusions===
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What did you achieve over the course of your semester?
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===Safety===
 
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What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
 
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===Attributions===
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Who worked on what?
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===Human Practices===
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What impact does/will your project have on the public? 
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===Fun!===
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What was your favorite team snack?? Have a picture of your team mascot?
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Latest revision as of 14:25, 20 June 2014