Lab Protocol Successes - Team OLeSsence
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- Successful completion of the 3A Protocols, including: |
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*Need to build gel illuminator next year |
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<li><a href="#">Project</a> | <li><a href="#">Project</a> | ||
<ul> | <ul> | ||
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<li><a> </a></li> | <li><a> </a></li> | ||
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<li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Project.html">Description</a></li> | <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Project.html">Description</a></li> | ||
<li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Timeline">Timeline</a></li> | <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Timeline">Timeline</a></li> | ||
+ | <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Lab_Successes.html">Lab Successes</a></li> | ||
<li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Lab_Notebook.html">Lab Notebook</a></li> | <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Lab_Notebook.html">Lab Notebook</a></li> | ||
</ul> | </ul> | ||
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</header> | </header> | ||
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<article id="main"> | <article id="main"> | ||
<h2> </h2> | <h2> </h2> | ||
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<td><ul> | <td><ul> | ||
- | <li align="left">- Mastered use of pressure-steam autoclave for all sterilization/decontamination</li> | + | <li align="left"><p align="left">- Mastered use of pressure-steam autoclave for all sterilization/decontamination</p></li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
+ | <tr> | ||
+ | <td><ul> | ||
+ | <p align="left">- Successful completion of the 3A Protocols, including:<br> | ||
+ | </ul></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><blockquote> | ||
+ | <blockquote> | ||
+ | <p align="left">- Restriction digest of plasmids<br> | ||
+ | - Gel electrophoresis of plasmid digest (NB: No evidence of DNA on gel)<br> | ||
+ | - Ligation protocol </p> | ||
+ | </blockquote> | ||
+ | </blockquote></td> | ||
+ | </tr> | ||
<tr> | <tr> | ||
<td><ul> | <td><ul> | ||
- | <li align="left">- Routinely preparing our own LB-agar plates (with and without antibiotic) and LB broth for cell culture.</li> | + | <li align="left"><p align="left">- Routinely preparing our own LB-agar plates (with and without antibiotic) and LB broth for cell culture.</p></li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
- | <p><img src=" | + | <p><img src="https://static.igem.org/mediawiki/2014hs/1/11/OLS_PROTOCOL_Image1-16.jpg" width="400" height="296" alt=""/></p> |
<table width="100%" border="0" cellspacing="1" cellpadding="10"> | <table width="100%" border="0" cellspacing="1" cellpadding="10"> | ||
<tr> | <tr> | ||
<td><ul> | <td><ul> | ||
- | <li align="left">- Successful plating techniques for E. coli – routinely growing single colonies free from contamination using aseptic technique.</li> | + | <li align="left"><p align="left">- Successful plating techniques for E. coli – routinely growing single colonies free from contamination using aseptic technique.</p></li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
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<table width="100%" border="0" cellspacing="1" cellpadding="10"> | <table width="100%" border="0" cellspacing="1" cellpadding="10"> | ||
<tr> | <tr> | ||
- | <td><img src=" | + | <td><img src="https://static.igem.org/mediawiki/2014hs/b/bf/OLS_PROTOCOL_Image2-19.jpg" width="477" height="358" alt=""/></td> |
- | <td><img src=" | + | <td><img src="https://static.igem.org/mediawiki/2014hs/e/ee/OLS_PROTOCOL_Image3-22.jpg" width="477" height="358" alt=""/></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><img src=" | + | <td><img src="https://static.igem.org/mediawiki/2014hs/5/55/OLS_PROTOCOL_Image4-25.jpg" width="477" height="356" alt=""/></td> |
- | <td><img src=" | + | <td><img src="https://static.igem.org/mediawiki/2014hs/5/5f/OLS_PROTOCOL_Image5-28.jpg" width="477" height="356" alt=""/></td> |
</tr> | </tr> | ||
</table> | </table> | ||
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<tr> | <tr> | ||
<td><ul> | <td><ul> | ||
- | <li align="left">- Successfully growing cell cultures from individual colonies in DIY shaker/incubator</li> | + | <li align="left"><p align="left">- Successfully growing cell cultures from individual colonies in DIY shaker/incubator</p></li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
- | + | <p><img src="https://static.igem.org/mediawiki/2014hs/b/be/OLS_PROTOCOL_Image6-31.jpg" width="477" height="356" alt=""/></p> | |
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<table width="100%" border="0" cellspacing="1" cellpadding="10"> | <table width="100%" border="0" cellspacing="1" cellpadding="10"> | ||
<tr> | <tr> | ||
<td><ul> | <td><ul> | ||
- | <li align="left">- Successfully grew E. coli cells (provided from iGEM) containing our parts (K098995 and J45119), including cell cultures.</li> | + | <li align="left"><p align="left">- Successfully grew E. coli cells (provided from iGEM) containing our parts (K098995 and J45119), including cell cultures.</p></li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><ul> | <td><ul> | ||
- | <li align="left">- Mastered use of DIY Dremelfuge (confirming RPM with photogate) to pellet DNA</li> | + | <li align="left"><p align="left">- Mastered use of DIY Dremelfuge (confirming RPM with photogate) to pellet DNA</p></li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><ul> | <td><ul> | ||
- | <li align="left">- Successfully mini-prepped both our parts to extract plasmid DNA</li> | + | <li align="left"><p align="left">- Successfully mini-prepped both our parts to extract plasmid DNA</p></li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
- | <p><img src=" | + | <p><img src="https://static.igem.org/mediawiki/2014hs/9/9a/OLS_PROTOCOL_Image8-36.jpg" width="400" height="301" alt=""/></p> |
<table width="100%" border="0" cellspacing="1" cellpadding="10"> | <table width="100%" border="0" cellspacing="1" cellpadding="10"> | ||
<tr> | <tr> | ||
<td><ul> | <td><ul> | ||
- | <li align="left"> - Successfully used restriction digest protocols to cut our plasmids of interest, confirming presence of DNA strands of expected size via gel electrophoresis</li> | + | <li align="left"><p align="left">- Successfully used restriction digest protocols to cut our plasmids of interest, confirming presence of DNA strands of expected size via gel electrophoresis</p></li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
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<table width="100%" border="0" cellspacing="1" cellpadding="10"> | <table width="100%" border="0" cellspacing="1" cellpadding="10"> | ||
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- | <td><img src=" | + | <td><img src="https://static.igem.org/mediawiki/2014hs/c/ce/OLS_PROTOCOL_Image9-39.jpg" width="477" height="366" alt=""/></td> |
- | <td><img src=" | + | <td><img src="https://static.igem.org/mediawiki/2014hs/3/3e/OLS_PROTOCOL_Image10-42.jpg" width="477" height="358" alt=""/></td> |
</tr> | </tr> | ||
</table> | </table> | ||
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<td><ul> | <td><ul> | ||
- | <li align="left">- Successfully transformed RFP plasmid into competent cells (prepped by our mentors) to grow RFP colonies.</li> | + | <li align="left"><p align="left">- Successfully transformed RFP plasmid into competent cells (prepped by our mentors) to grow RFP colonies.</p></li> |
</ul></td> | </ul></td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
- | <p><img src=" | + | <p><img src="https://static.igem.org/mediawiki/2014hs/0/03/OLS_PROTOCOL_Image11-45.jpg" width="569" height="425" alt=""/></p> |
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</table> | </table> | ||
</article> | </article> |
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- Successful completion of the 3A Protocols, including: |
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*Need to build gel illuminator next year |
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