Team:OLS Canmore AB CA/Lab Successes.html

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                           <li><a href="#">Project</a>
                           <li><a href="#">Project</a>
                               <ul>
                               <ul>
-
                                  <li><a>&nbsp;</a></li>
 
                                   <li><a>&nbsp;</a></li>
                                   <li><a>&nbsp;</a></li>
                                   <li><a>&nbsp;</a></li>
                                   <li><a>&nbsp;</a></li>
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                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Project.html">Description</a></li>
                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Project.html">Description</a></li>
                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Timeline">Timeline</a></li>
                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Timeline">Timeline</a></li>
 +
                                  <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Lab_Successes.html">Lab Successes</a></li>
                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Lab_Notebook.html">Lab Notebook</a></li>
                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Lab_Notebook.html">Lab Notebook</a></li>
                               </ul>
                               </ul>
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             </div>
             </div>
     </header>
     </header>
-
 
  <article id="main">
  <article id="main">
   <h2>&nbsp;</h2>
   <h2>&nbsp;</h2>
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       <tr>
       <tr>
         <td><ul>
         <td><ul>
-
           <li>- Mastered use of pressure-steam autoclave for all sterilization/decontamination</li>
+
           <li align="left"><p align="left">- Mastered use of pressure-steam autoclave for all sterilization/decontamination</p></li>
         </ul></td>
         </ul></td>
       </tr>
       </tr>
 +
<tr>
 +
    <td><ul>
 +
      <p align="left">- Successful completion of the 3A Protocols, including:<br>
 +
      </ul></p>
 +
    </td>
 +
  </tr>
 +
  <tr>
 +
    <td><blockquote>
 +
      <blockquote>
 +
        <p align="left">- Restriction digest of plasmids<br>
 +
          - Gel electrophoresis of plasmid digest (NB: No evidence of DNA on gel)<br>
 +
          - Ligation protocol </p>
 +
      </blockquote>
 +
    </blockquote></td>
 +
  </tr>
       <tr>
       <tr>
         <td><ul>
         <td><ul>
-
           <li>- Routinely preparing our own LB-agar plates (with and without antibiotic) and LB broth for cell culture.</li>
+
           <li align="left"><p align="left">- Routinely preparing our own LB-agar plates (with and without antibiotic) and LB broth for cell culture.</p></li>
         </ul></td>
         </ul></td>
       </tr>
       </tr>
       </table>
       </table>
-
       <p><img src="file:///Macintosh HD/Users/carterbehm/Desktop/image1-16.jpg" width="400" height="296" alt=""/></p>
+
       <p><img src="https://static.igem.org/mediawiki/2014hs/1/11/OLS_PROTOCOL_Image1-16.jpg" width="400" height="296" alt=""/></p>
       <table width="100%" border="0" cellspacing="1" cellpadding="10">
       <table width="100%" border="0" cellspacing="1" cellpadding="10">
       <tr>
       <tr>
         <td><ul>
         <td><ul>
-
           <li>- Successful plating techniques for E. coli – routinely growing single colonies free from contamination using aseptic technique.</li>
+
           <li align="left"><p align="left">- Successful plating techniques for E. coli – routinely growing single colonies free from contamination using aseptic technique.</p></li>
         </ul></td>
         </ul></td>
       </tr>
       </tr>
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     <table width="100%" border="0" cellspacing="1" cellpadding="10">
     <table width="100%" border="0" cellspacing="1" cellpadding="10">
   <tr>
   <tr>
-
     <td><img src="file:///Macintosh HD/Users/carterbehm/Desktop/image2-19.jpg" width="477" height="358" alt=""/></td>
+
     <td><img src="https://static.igem.org/mediawiki/2014hs/b/bf/OLS_PROTOCOL_Image2-19.jpg" width="477" height="358" alt=""/></td>
-
     <td><img src="file:///Macintosh HD/Users/carterbehm/Desktop/image3-22.jpg" width="477" height="358" alt=""/></td>
+
     <td><img src="https://static.igem.org/mediawiki/2014hs/e/ee/OLS_PROTOCOL_Image3-22.jpg" width="477" height="358" alt=""/></td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td><img src="file:///Macintosh HD/Users/carterbehm/Desktop/image4-25.jpg" width="477" height="356" alt=""/></td>
+
     <td><img src="https://static.igem.org/mediawiki/2014hs/5/55/OLS_PROTOCOL_Image4-25.jpg" width="477" height="356" alt=""/></td>
-
     <td><img src="file:///Macintosh HD/Users/carterbehm/Desktop/image5-28.jpg" width="477" height="356" alt=""/></td>
+
     <td><img src="https://static.igem.org/mediawiki/2014hs/5/5f/OLS_PROTOCOL_Image5-28.jpg" width="477" height="356" alt=""/></td>
   </tr>
   </tr>
</table>
</table>
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   <tr>
   <tr>
     <td><ul>
     <td><ul>
-
       <li>- Successfully growing cell cultures from individual colonies in DIY shaker/incubator</li>
+
       <li align="left"><p align="left">- Successfully growing cell cultures from individual colonies in DIY shaker/incubator</p></li>
     </ul></td>
     </ul></td>
   </tr>
   </tr>
</table>
</table>
-
<table width="100%" border="0" cellspacing="1" cellpadding="10">
+
     <p><img src="https://static.igem.org/mediawiki/2014hs/b/be/OLS_PROTOCOL_Image6-31.jpg" width="477" height="356" alt=""/></p>
-
  <tr>
+
-
     <td><img src="#" width="477" height="356" alt=""/></td>
+
-
    <td><img src="file:///Macintosh HD/Users/carterbehm/Desktop/image6-31.jpg" width="477" height="356" alt=""/></td>
+
-
  </tr>
+
-
</table>
+
<table width="100%" border="0" cellspacing="1" cellpadding="10">
<table width="100%" border="0" cellspacing="1" cellpadding="10">
   <tr>
   <tr>
     <td><ul>
     <td><ul>
-
       <li>- Successfully grew E. coli cells (provided from iGEM) containing our parts (K098995 and J45119), including cell cultures.</li>
+
       <li align="left"><p align="left">- Successfully grew E. coli cells (provided from iGEM) containing our parts (K098995 and J45119), including cell cultures.</p></li>
     </ul></td>
     </ul></td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td><ul>
     <td><ul>
-
       <li>- Mastered use of DIY Dremelfuge (confirming RPM with photogate) to pellet DNA</li>
+
       <li align="left"><p align="left">- Mastered use of DIY Dremelfuge (confirming RPM with photogate) to pellet DNA</p></li>
     </ul></td>
     </ul></td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td><ul>
     <td><ul>
-
       <li>- Successfully mini-prepped both our parts to extract plasmid DNA</li>
+
       <li align="left"><p align="left">- Successfully mini-prepped both our parts to extract plasmid DNA</p></li>
     </ul></td>
     </ul></td>
   </tr>
   </tr>
</table>
</table>
-
     <p><img src="file:///Macintosh HD/Users/carterbehm/Desktop/image8-36.jpg" width="400" height="301" alt=""/></p>
+
     <p><img src="https://static.igem.org/mediawiki/2014hs/9/9a/OLS_PROTOCOL_Image8-36.jpg" width="400" height="301" alt=""/></p>
     <table width="100%" border="0" cellspacing="1" cellpadding="10">
     <table width="100%" border="0" cellspacing="1" cellpadding="10">
   <tr>
   <tr>
     <td><ul>
     <td><ul>
-
       <li> - Successfully used restriction digest protocols to cut our plasmids of interest, confirming presence of DNA strands of expected size via gel electrophoresis</li>
+
       <li align="left"><p align="left">- Successfully used restriction digest protocols to cut our plasmids of interest, confirming presence of DNA strands of expected size via gel electrophoresis</p></li>
     </ul></td>
     </ul></td>
   </tr>
   </tr>
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<table width="100%" border="0" cellspacing="1" cellpadding="10">
<table width="100%" border="0" cellspacing="1" cellpadding="10">
   <tr>
   <tr>
-
     <td><img src="file:///Macintosh HD/Users/carterbehm/Desktop/image9-39.jpg" width="477" height="366" alt=""/></td>
+
     <td><img src="https://static.igem.org/mediawiki/2014hs/c/ce/OLS_PROTOCOL_Image9-39.jpg" width="477" height="366" alt=""/></td>
-
     <td><img src="file:///Macintosh HD/Users/carterbehm/Desktop/image10-42.jpg" width="477" height="358" alt=""/></td>
+
     <td><img src="https://static.igem.org/mediawiki/2014hs/3/3e/OLS_PROTOCOL_Image10-42.jpg" width="477" height="358" alt=""/></td>
   </tr>
   </tr>
</table>
</table>
<table width="100%" border="0" cellspacing="1" cellpadding="10">
<table width="100%" border="0" cellspacing="1" cellpadding="10">
   <tr>
   <tr>
-
     <td>*Need to build gel illuminator next year</td>
+
     <td align="left">*Need to build gel illuminator next year</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td><ul>
     <td><ul>
-
       <li>- Successfully transformed RFP plasmid into competent cells (prepped by our mentors) to grow RFP colonies.</li>
+
       <li align="left"><p align="left">- Successfully transformed RFP plasmid into competent cells (prepped by our mentors) to grow RFP colonies.</p></li>
     </ul></td>
     </ul></td>
   </tr>
   </tr>
</table>
</table>
-
<p><img src="file:///Macintosh HD/Users/carterbehm/Desktop/image11-45.jpg" width="569" height="425" alt=""/></p>
+
<p><img src="https://static.igem.org/mediawiki/2014hs/0/03/OLS_PROTOCOL_Image11-45.jpg" width="569" height="425" alt=""/></p>
<table width="100%" border="0" cellspacing="1" cellpadding="10">
<table width="100%" border="0" cellspacing="1" cellpadding="10">
   <tr>
   <tr>
-
     <td>=============================================================================</td>
+
     <td>
-
  </tr>
+
</td>
-
  <tr>
+
-
    <td><blockquote>
+
-
      <p>- Successful completion of the 3A Protocols, including:<br>
+
-
      </p>
+
-
    </blockquote></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td><blockquote>
+
-
      <blockquote>
+
-
        <p>- Restriction digest of plasmids<br>
+
-
          - Gel electrophoresis of plasmid digest (NB: No evidence of DNA on gel)<br>
+
-
          - Ligation protocol </p>
+
-
      </blockquote>
+
-
    </blockquote></td>
+
   </tr>
   </tr>
 +
</table>
</table>
   </article>
   </article>

Latest revision as of 22:24, 20 June 2014

Project

 

Lab Protocol Successes - Team OLeSsence

 

  • - Mastered use of pressure-steam autoclave for all sterilization/decontamination

    - Successful completion of the 3A Protocols, including:

- Restriction digest of plasmids
- Gel electrophoresis of plasmid digest (NB: No evidence of DNA on gel)
- Ligation protocol

  • - Routinely preparing our own LB-agar plates (with and without antibiotic) and LB broth for cell culture.

  • - Successful plating techniques for E. coli – routinely growing single colonies free from contamination using aseptic technique.

  • - Successfully growing cell cultures from individual colonies in DIY shaker/incubator

  • - Successfully grew E. coli cells (provided from iGEM) containing our parts (K098995 and J45119), including cell cultures.

  • - Mastered use of DIY Dremelfuge (confirming RPM with photogate) to pellet DNA

  • - Successfully mini-prepped both our parts to extract plasmid DNA

  • - Successfully used restriction digest protocols to cut our plasmids of interest, confirming presence of DNA strands of expected size via gel electrophoresis

*Need to build gel illuminator next year
  • - Successfully transformed RFP plasmid into competent cells (prepped by our mentors) to grow RFP colonies.

2014 Our Lady of the Snows iGEM