Team:OLS Canmore AB CA/Lab Successes.html

• - Mastered use of pressure-steam autoclave for all sterilization/decontamination

- Successful completion of the 3A Protocols, including:

- Restriction digest of plasmids
- Gel electrophoresis of plasmid digest (NB: No evidence of DNA on gel)
- Ligation protocol

• - Routinely preparing our own LB-agar plates (with and without antibiotic) and LB broth for cell culture.

• - Successful plating techniques for E. coli – routinely growing single colonies free from contamination using aseptic technique.

• - Successfully growing cell cultures from individual colonies in DIY shaker/incubator

• - Successfully grew E. coli cells (provided from iGEM) containing our parts (K098995 and J45119), including cell cultures.

• - Mastered use of DIY Dremelfuge (confirming RPM with photogate) to pellet DNA

• - Successfully mini-prepped both our parts to extract plasmid DNA

• - Successfully used restriction digest protocols to cut our plasmids of interest, confirming presence of DNA strands of expected size via gel electrophoresis

• - Successfully transformed RFP plasmid into competent cells (prepped by our mentors) to grow RFP colonies.

- Successful completion of the 3A Protocols, including:

- Restriction digest of plasmids
- Gel electrophoresis of plasmid digest (NB: No evidence of DNA on gel)
- Ligation protocol