Team:OLS Canmore AB CA/Abstract.html

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                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Abstract.html">Abstract</a></li>
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                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Project.html">Description</a></li>
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                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Timeline">Timeline</a></li>
                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Timeline">Timeline</a></li>
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                                  <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Lab_Successes.html">Lab Successes</a></li>
                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Lab_Notebook.html">Lab Notebook</a></li>
                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Lab_Notebook.html">Lab Notebook</a></li>
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                            <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Human_Practices.html">Human Practices</a>
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                          <li><a href="#">Human Practices</a>
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                                   <li><a href="https://www.facebook.com/OLeSsence" target="_blank"><img src="https://static.igem.org/mediawiki/2014hs/5/54/OLS_Facebook_button.png" width="45" height="45" alt=""/></a></li>
                                   <li><a href="https://www.facebook.com/OLeSsence" target="_blank"><img src="https://static.igem.org/mediawiki/2014hs/5/54/OLS_Facebook_button.png" width="45" height="45" alt=""/></a></li>
                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Bioethics">Ethics</a></li>
                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Bioethics">Ethics</a></li>
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                           <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Safety.html">Safety</a>
                           <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Safety.html">Safety</a>
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                           <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/About_Us.html">About Us</a>
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                           <li><a href="#">About Us</a>
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                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/The_Team.html">The Team</a></li>
                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/The_Team.html">The Team</a></li>
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                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Sponsors.html">Sponsors</a></li>
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                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Sponsor.html">Sponsors</a></li>
                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Attributions.html">Attributions</a></li>
                                   <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/Attributions.html">Attributions</a></li>
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                                  <li><a href="https://2014hs.igem.org/Team:OLS_Canmore_AB_CA/About_Us.html">Contact Us</a></li>
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  <h2>&nbsp;</h2>
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<article id="main">
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  <h2>Our Project</h2>
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    <h2>&nbsp;</h2>
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<p>&nbsp;</p>
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    <h2>Abstract</h2>
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  <p>The objective for our school&rsquo;s first iGEM project is to create a heat activated biosensor. When exposed to temperatures of 42 degrees Celsius or higher, our biosensor will emit a wintergreen smell. &nbsp;The experience from this project will also serve as the building block for our long-term vision.</p>
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    <p>&nbsp;</p>
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         <td align="center"><p><strong>Heat-induced Olfactory Biosensor</strong></p>
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         <td><img src="https://static.igem.org/mediawiki/2014hs/0/01/OLS_diagram-1.png" width="470" height="310" alt=""/></td>
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            <p align="left">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;As Our Lady of the Snow’s first iGEM team, we have decided upon creating a heat-induced olfactory biosensor using <em>E. coli</em> as a chassis. Constructed by placing a wintergreen odor enzyme generator downstream from a heat sensitive promoter, the bacteria should emit a noticeable wintergreen smell starting near a human body temperature of 37°C. As the temperature increases the heat sensitive promoter gradually decreases the effect of the repressor to allow for maximum wintergreen enzyme generation around 42°C. The creation of this biobrick will serve as a first step towards a multiple input bio-sensing system. Such a system may include the input of heat and different wavelengths for activation. Both pursuits will help achieve the goal of using bacteria in increasingly complex circuits to more effectively sense the environment.</p>
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        <td colspan="2"><strong>Parts BBa_K098995/BBa_J45119</strong></td>
 
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  <p>To create this biosensor, we will place a wintergreen odor enzyme generator (BBa_J45119) downstream from a heat sensitive promoter (BBa_KO98995). The wintergreen odor generator will only work when salicylic acid is present. We believe our project could be used to detect severe fevers, and would also be used to educate our community about the benefits of genetic engineering. </p>
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<p>&nbsp;</p>
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  <figure><img src="https://static.igem.org/mediawiki/2014hs/7/71/OLS_diagram-3.png" width="350" height="471" alt=""/>
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<p>&nbsp;</p>
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    <figcaption>Project Plan</figcaption>
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<p>&nbsp;</p>
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<p>&nbsp;</p>
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  <p>Our team&rsquo;s long-term project consists of creating a thermal-wavelength biosensor. The project described in the paragraphs above will be our goal for the semester, and will help us gain the skills and knowledge to pursue more complex projects—such as a thermal-wavelength biosensor. To create this biosensor, we will string together BBa_K909001, BBa_K098995, BBa_K909003, BBa_R0082, and BBa_J45119, in that order. This will also emit a wintergreen odor, but will require heat as well as to be in the presence of a specific wavelength of light. </p>
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<p>&nbsp;</p>
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  <figure><img src="https://static.igem.org/mediawiki/2014hs/4/40/OLS_diagram-4.png" width="400" height="210" alt=""/>
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<p>&nbsp;</p>
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    <figcaption>Long-term Project</figcaption>
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<p>&nbsp;</p>
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<p>&nbsp;</p>
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Latest revision as of 20:44, 19 June 2014

Safety

 

Abstract

 

Heat-induced Olfactory Biosensor

     As Our Lady of the Snow’s first iGEM team, we have decided upon creating a heat-induced olfactory biosensor using E. coli as a chassis. Constructed by placing a wintergreen odor enzyme generator downstream from a heat sensitive promoter, the bacteria should emit a noticeable wintergreen smell starting near a human body temperature of 37°C. As the temperature increases the heat sensitive promoter gradually decreases the effect of the repressor to allow for maximum wintergreen enzyme generation around 42°C. The creation of this biobrick will serve as a first step towards a multiple input bio-sensing system. Such a system may include the input of heat and different wavelengths for activation. Both pursuits will help achieve the goal of using bacteria in increasingly complex circuits to more effectively sense the environment.

 

 

 

 

 

 

 

 

 

2014 Our Lady of the Snows iGEM