Team:NGSS TR/results.html
From 2014hs.igem.org
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<h2><span class="gfdg">1.SUCCESSFUL CLONING</span></h2> | <h2><span class="gfdg">1.SUCCESSFUL CLONING</span></h2> | ||
<p><br /> | <p><br /> | ||
- | We did the transformation of our composite part (OmpA-linker-SpeB_Cleavage_Site-XylE) | + | We did the transformation of our composite part (OmpA-linker-SpeB_Cleavage_Site-XylE). We applied our regular procedure (see procedures under the lab button) to E.coli colonies that we have prepared before. Electrophoresis results showed that our gene is produced by the colonies and it is in the correct range (1500-2000 bp). After the repetitions of the same procedure, we agreed that cloning was successful.</p> |
<img src="https://static.igem.org/mediawiki/2014hs/7/72/Electro.jpg"/> | <img src="https://static.igem.org/mediawiki/2014hs/7/72/Electro.jpg"/> | ||
- | <p> | + | <p> Image:Electrophoresis of OmpA-linker-SpeB_Cleavage_Site-XylE</p> |
<p> </p> | <p> </p> | ||
<h2><span class="gfdg">2.SDS PAGE EXPERIMENT</span></h2> | <h2><span class="gfdg">2.SDS PAGE EXPERIMENT</span></h2> | ||
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<p>In order to check whether our enzyme is working or not and also to control if the proteins are producing, we made the SDS PAGE experiment. We showed that our enzyme is working and our construct is produced. After the experiment of lysate the band that we want to see is nearly 53 kda long.</p> | <p>In order to check whether our enzyme is working or not and also to control if the proteins are producing, we made the SDS PAGE experiment. We showed that our enzyme is working and our construct is produced. After the experiment of lysate the band that we want to see is nearly 53 kda long.</p> | ||
<img src="https://static.igem.org/mediawiki/2014hs/b/b4/Sds1.jpg"/> | <img src="https://static.igem.org/mediawiki/2014hs/b/b4/Sds1.jpg"/> | ||
+ | <p> 1)IgG 2)IgG with PBS+Mercaptoethanol 3)IgG with SpeB+PBS+Mercaptoethanol 4)marker 5)Compatent E. Coli 6)Bacterial lysate (2,5 ul) 7)Bacterial lysate (5 ul) 8)Bacterial lysate (10 ul) 9)Bacterial lysate (20 ul) 10)Marker</p> | ||
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<p>Our system was working, we decided to do color change experiment. </p> | <p>Our system was working, we decided to do color change experiment. </p> | ||
<p> </p> | <p> </p> |
Revision as of 02:17, 21 June 2014
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