Team:NGSS TR/lb.html

From 2014hs.igem.org

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         </section>
         </section>
       </div>
       </div>
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<blockquote>
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  <p>&nbsp;</p>
 +
  <blockquote>
 +
    <h1>PROTOCOLS</h1>
 +
    <blockquote>
 +
      <h2 class="gfdg">5.LB PREPARATION</h2>
 +
      <blockquote>
 +
        <p><strong><em><u>LB-BROTH</u></em></strong><br />
 +
            <strong>Materials:</strong><br />
 +
            <strong>- LB-Broth(chemical storage)</strong><br />
 +
            <strong>- Sterile dH2O</strong><br />
 +
            <strong>- Flask&nbsp;</strong><br />
 +
            <strong>- Lab skale</strong><br />
 +
            <strong>- Liquid autoclave</strong><br />
 +
            <strong>- Alumininum folio</strong><br />
 +
          <strong>- Antibiotic</strong></p>
 +
        <p><a name="_GoBack" id="_GoBack"></a><strong>Experiment:</strong><br />
 +
            <strong>1. In a steril environment, the tare of  the container should be measured and subtracted from the overall weight.</strong><br />
 +
            <strong>2. 4 grams of LB Broth is put in the container.</strong><br />
 +
            <strong>3. Add 200 ml distilled water to graduated  cyclindar.</strong><br />
 +
            <strong>4. These two are mixed in a beaker.</strong><br />
 +
            <strong>5. When you are opening the beaker be sure  that it doesn&rsquo;t contact with air.</strong><br />
 +
            <strong>6. Autoclave tape is sticked on to the  aliminium.</strong><br />
 +
            <strong>7. The beaker is placed in to the  autoclave machine.</strong><br />
 +
            <strong>8. Autoclave machine has to have distilled  water or demineralized water. The water level in the autoclave machine has to  be a little higher than the liquid level of the beaker.</strong><br />
 +
            <strong>9. Then start the 90 min.</strong><br />
 +
          <strong>10. Take out the beaker and add antibiotics  if required.</strong></p>
 +
        <h2><strong>Attention!</strong><br />
 +
            <strong>v Don&rsquo;t be far away fire.</strong><br />
 +
          <strong>v Don&rsquo; forget to write date &nbsp;</strong></h2>
 +
        <p>&nbsp;</p>
 +
        <p><strong><em><u>LB-Agar</u></em></strong><br />
 +
            <strong>Materials:</strong><br />
 +
            <strong>- LB-Agar(chemical storage)</strong><br />
 +
            <strong>- Sterile dH2O</strong><br />
 +
            <strong>- Flask&nbsp;</strong><br />
 +
            <strong>- Liquid autoclave</strong><br />
 +
            <strong>- Aluminium folio</strong><br />
 +
            <strong>- Antibiotic</strong><br />
 +
            <strong>- Empty plates</strong><br />
 +
          <strong>- Parafilm</strong></p>
 +
        <p><strong>Experiment:</strong><br />
 +
            <strong>1. In a steril environment, the tare of  the container should be measured and subtracted from the overall weight.</strong><br />
 +
            <strong>2. 7 grams of LB Agar is put in the container.(If  there is no LB agar, add the 4  gram LB broth and 2,7 gram agar.)</strong><br />
 +
            <strong>3. Add 200 ml distilled water to graduated  cyclindar.</strong><br />
 +
            <strong>4. These two are mixed in a beaker.</strong><br />
 +
            <strong>5. When you are opening the beaker be sure  that it doesn&rsquo;t contact with air.</strong><br />
 +
            <strong>6. Autoclave tape is sticked on to the  aliminium.</strong><br />
 +
            <strong>7. The beaker is placed in to the  autoclave machine.</strong><br />
 +
            <strong>8. Autoclave machine has to have distilled  water or demineralized water. The water level in the autoclave machine has to  be a little higher than the liquid level of the beaker.</strong><br />
 +
            <strong>9. Then start the 90 min.</strong><br />
 +
            <strong>10. Take out the beaker and add antibiotics  if required.</strong><br />
 +
            <strong>Attention!</strong><br />
 +
            <strong>v Don&rsquo;t be far away fire.</strong><br />
 +
            <strong>v Don&rsquo; forget to write the date.</strong><br />
 +
          <strong>v Don't forget add the amtibiotics</strong></p>
 +
      </blockquote>
 +
    </blockquote>
 +
    </p>
 +
</blockquote>

Latest revision as of 11:48, 20 June 2014

 

PROTOCOLS

5.LB PREPARATION

LB-BROTH
Materials:
- LB-Broth(chemical storage)
- Sterile dH2O
- Flask 
- Lab skale
- Liquid autoclave
- Alumininum folio
- Antibiotic

Experiment:
1. In a steril environment, the tare of the container should be measured and subtracted from the overall weight.
2. 4 grams of LB Broth is put in the container.
3. Add 200 ml distilled water to graduated cyclindar.
4. These two are mixed in a beaker.
5. When you are opening the beaker be sure that it doesn’t contact with air.
6. Autoclave tape is sticked on to the aliminium.
7. The beaker is placed in to the autoclave machine.
8. Autoclave machine has to have distilled water or demineralized water. The water level in the autoclave machine has to be a little higher than the liquid level of the beaker.
9. Then start the 90 min.
10. Take out the beaker and add antibiotics if required.

Attention!
v Don’t be far away fire.
v Don’ forget to write date  

 

LB-Agar
Materials:
- LB-Agar(chemical storage)
- Sterile dH2O
- Flask 
- Liquid autoclave
- Aluminium folio
- Antibiotic
- Empty plates
- Parafilm

Experiment:
1. In a steril environment, the tare of the container should be measured and subtracted from the overall weight.
2. 7 grams of LB Agar is put in the container.(If there is no LB agar, add the 4 gram LB broth and 2,7 gram agar.)
3. Add 200 ml distilled water to graduated cyclindar.
4. These two are mixed in a beaker.
5. When you are opening the beaker be sure that it doesn’t contact with air.
6. Autoclave tape is sticked on to the aliminium.
7. The beaker is placed in to the autoclave machine.
8. Autoclave machine has to have distilled water or demineralized water. The water level in the autoclave machine has to be a little higher than the liquid level of the beaker.
9. Then start the 90 min.
10. Take out the beaker and add antibiotics if required.
Attention!
v Don’t be far away fire.
v Don’ forget to write the date.
v Don't forget add the amtibiotics

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