Team:Montgomery Cougars NJUSA/Team Log

From 2014hs.igem.org

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(Team Log: mar 21)
 
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{{Montgomery_Cougars_NJUSA/Layout:Two_Column}}
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=Team Log=
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The Montgomery iGEM's teams documentation of activities, labs, and lectures for each day.
 +
{{anchor|22nov2013}}
{| class="wikitable" style="text-align:left; width:100%; height:200px;"
{| class="wikitable" style="text-align:left; width:100%; height:200px;"
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!colspan="2" style="background: #ffdead;" |December 13th 2013
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!colspan="2" style="background: #ffdead;" |November 22nd 2013
|-
|-
! width="140" | Mission Statement
! width="140" | Mission Statement
-
|Start team organization and keep progressing with the fundraising/sponsorship efforts. Assemble board 2013/2014.
+
| We wanted to implement a new attendance sign-in setup, announce participation consent forms (principle consent form), and organize sub-teams.  
|-
|-
! width="140" |Meeting Overview
! width="140" |Meeting Overview
-
| We began the meeting by announcing 2013/2014 officers followed by showing a video about synthetic biology to help members understand the importance of the club. The club will consist of the board as well as two sub-teams. The board collected emails and planned for fundraising and registration.
+
| We started by electronically taking attendance and introducing the two sub-teams. All members were allowed to choose their sub-teams (may cut down R&D later). We talked about fundraising because of the December 31st deadline of registration and payment.
 +
Possible fundraising ideas:
 +
- Hot chocolate
 +
- Snow-hat sales
 +
- ShopRite Bagging
 +
It was also the last “introductory” meeting. We announced the deadline for leadership applications due Wednesday, November 27 and reminded members to fill out consent forms.
|-
|-
! width="140" |Goals/Tasks for Next Meeting
! width="140" |Goals/Tasks for Next Meeting
-
| We will start teaching the basics of synthetic biology, organizing the ShopRite bagging fundraiser, creating online accounts for everyone, and cleaning up attendance lists.  
+
| We planned to come up with an idea for fundraising and collect all the participation consent forms.
 +
 
 +
Finished principle consent form
 +
|-
 +
! width="140" |Other Notes
 +
| If members tried to look for sponsors, they had to send iGEM write-ups to them.
 +
We found sponsors quickly and focused on small companies in Montgomery.
 +
In addition, we checked with the supervisor to see whether he had any ideas or grants.  
|-
|-
|}
|}
 +
{{anchor|13dec2013}}
{| class="wikitable" style="text-align:left; width:100%; height:200px;"
{| class="wikitable" style="text-align:left; width:100%; height:200px;"
|-
|-
-
!colspan="2" style="background: #ffdead;" |November 22nd 2013
+
!colspan="2" style="background: #ffdead;" |December 13th 2013
|-
|-
! width="140" | Mission Statement
! width="140" | Mission Statement
-
| Implement new attendance sign-in setup; announce participation consent forms (principle consent form); organize sub-teams
+
|We aimed to effectively organize the team and keep progressing with the fundraising and sponsorship efforts, as well as assemble the 2013-2014 board members.
|-
|-
! width="140" |Meeting Overview
! width="140" |Meeting Overview
-
| We started by electronically taking attendance and introducing the two sub-teams. All members were allowed to choose their sub-teams (may cut down R&D later). Talked about fundraising because deadline -- December 31st
+
| We began the meeting by announcing the 2013/2014 board members, followed by showing a video about synthetic biology to help members understand the importance of the team. The team will consist of the board as well as two sub-teams. The board collected emails and planned for fundraising and registration.
-
Hot chocolate
+
-
Snow-hat sales
+
-
ShopRite Bagging
+
-
Last “introductory” meeting
+
-
Leadership applications due Wed, 11/27, remind members to fill out consent forms
+
|-
|-
! width="140" |Goals/Tasks for Next Meeting
! width="140" |Goals/Tasks for Next Meeting
-
| Come up with an idea for fundraising..  
+
| We hoped to start teaching the basics of synthetic biology, organizing the ShopRite bagging fundraiser, creating online accounts for everyone, and cleaning up attendance lists.  
-
Gather all participation consent forms
+
-
 
+
-
Finish principle consent form
+
|-
|-
-
! width="140" |Other Notes
+
|}
-
| If members try to look for sponsors, send iGEM write-up to them
+
-
Find sponsors quickly; focus on small companies in Montgomery
+
{{anchor|20dec2013}}
-
 
+
-
Check with supervisor to see whether he has any ideas/grants
+
-
|-
+
-
|}
+
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{| class="wikitable" style="text-align:left; width:100%; height:200px;"
|-
|-
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|-
|-
! width="140" |Mission Statement
! width="140" |Mission Statement
-
|Design biological circuit from DNA sequence
+
|We designed biological circuit from DNA sequence
|-
|-
! width="140" |Meeting Overview
! width="140" |Meeting Overview
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|-
|}
|}
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{{anchor|17jan2014}}
{| class="wikitable" style="text-align:left; width:100%; height:200px;"
{| class="wikitable" style="text-align:left; width:100%; height:200px;"
|-
|-
-
!colspan="2" style="background: #ffdead;" |January 17th 2013
+
!colspan="2" style="background: #ffdead;" |January 17th 2014
|-
|-
! width="140" | Mission Statement
! width="140" | Mission Statement
-
| To learn information and become organized for the future.
+
| We learned information and became organized for the future.
|-
|-
! width="140" |Meeting Overview
! width="140" |Meeting Overview
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{{anchor|24jan2014}}
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{{anchor|31jan2014}}
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|-
|-
! width="140" | Mission Statement
! width="140" | Mission Statement
-
| To review laboratory technique for upcoming labs and to learn the correct procedures with respect to safety, bacterial transformation and general lab conduct.
+
| Go over lab technique.
|-
|-
! width="140" |Meeting Overview
! width="140" |Meeting Overview
-
Reviewed and analyzed pGLO labs involving 2 test tubes(+pGLO and -pGLO)
+
Aseptic technique, review pGLO lab.
-
Learned about aseptic technique
+
-
How to clean with a Bunsen Burner(if metal use plastic loop.  
+
How to clean with a Bunsen Burner(if metal use plastic loop).  
|-
|-
! width="140" |Goals/Tasks for Next Meeting
! width="140" |Goals/Tasks for Next Meeting
-
| To have Dr. Fishman, a calculus teacher at our school, come in and explain mathematical modeling.
+
| We accomplished the pGLO lab successfully without contamination and learned to make our own plasmids to put into bacteria.
 +
|-
 +
|}
 +
 
 +
{{anchor|3feb2014}}
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{| class="wikitable" style="text-align:left; width:100%; height:200px;"
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|-
 +
!colspan="2" style="background: #ffdead;" | February 3rd 2014
 +
|-
 +
! width="140" | Mission Statement
 +
| Continue preparations for pGLO Lab.
 +
|-
 +
! width="140" |Meeting Overview
 +
| We continued preparations for the pGLO lab. Members reviewed how the the lab procedure and also reviewed how the plasmid would be affected during the process.
 +
|-
 +
! width="140" |Goals/Tasks for Next Meeting
 +
| There was a complication with the materials which didn’t allow us to do the lab at the next meeting. 
 +
|-
 +
|}
 +
 
 +
{{anchor|20feb2014}}
 +
{| class="wikitable" style="text-align:left; width:100%; height:200px;"
 +
|-
 +
!colspan="2" style="background: #ffdead;" |February 20th 2014
 +
|-
 +
! width="140" | Mission Statement
 +
| We changed the dynamics of iGEM and administered the lab practical.
 +
|-
 +
! width="140" |Meeting Overview
 +
|  We collected t-shirt money, and Robert gave a speech on dynamics. There was a Q&A session about the lab practical and iGEM
 +
 
 +
|-
 +
! width="140" |Goals/Tasks for Next Meeting
 +
| Prepare for pGLO lab
|-
|-
! width="140" |Other Notes
! width="140" |Other Notes
-
The T-shirt are coming on April 8th and the ShopRite bagging is confirmed. We plan to finish mapping the enzyme pathways and lab procedure by the end of April or at the latest early May so that we may test throughout the month of May.
+
Shoprite bagging in March
 +
Need to take yearbook photo soon.
 +
Meetings are mandatory.
 +
|-
 +
|}
 +
 
 +
{{anchor|7mar2014}}
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{| class="wikitable" style="text-align:left; width:100%; height:200px;"
 +
|-
 +
!colspan="2" style="background: #ffdead;" |March 7th 2014
 +
|-
 +
! width="140" | Mission Statement
 +
| We finished the pGLO lab today. We organized the operations team.
 +
|-
 +
! width="140" |Meeting Overview
 +
| We formatted team organization, finalize acne project description, and administered the practice lab documentations. Board member headshots were also taken.
 +
 
 +
|-
 +
! width="140" |Goals/Tasks for Next Meeting
 +
| We started DNA ligation lessons and researched more on the acne project.
 +
|-
 +
! width="140" |Other Notes
 +
|  We started website structure.
 +
|-
 +
|}
 +
 
 +
{{anchor|14mar2014}}
 +
{| class="wikitable" style="text-align:left; width:100%; height:200px;"
 +
|-
 +
!colspan="2" style="background: #ffdead;" |March 14th 2014
 +
|-
 +
! width="140" | Mission Statement
 +
| We played an icebreaker to unify the team, went over lab results, and did post-lab calculations.
 +
|-
 +
! width="140" |Meeting Overview
 +
|  We went over t-shirt sizes, reviewed team members' names and favorite Pokemon, went over results from pGLO lab, counted number of colonies growing under UV light, and calculated transformation efficiency.
 +
 
 +
|-
 +
! width="140" |Goals/Tasks for Next Meeting
 +
| We will learn about DNA ligation.
 +
|-
 +
! width="140" |Other Notes
 +
|  Although some students failed, they worked hard on the lab.
 +
|-
 +
|}
 +
 
 +
 
 +
{{anchor|14mar2014}}
 +
{| class="wikitable" style="text-align:left; width:100%; height:200px;"
 +
|-
 +
!colspan="2" style="background: #ffdead;" |March 14th 2014
 +
|-
 +
! width="140" | Mission Statement
 +
| We played an icebreaker to unify the team, went over lab results, and did post-lab calculations.
 +
|-
 +
! width="140" |Meeting Overview
 +
|  We went over t-shirt sizes, reviewed team members' names and favorite Pokemon, went over results from pGLO lab, counted number of colonies growing under UV light, and calculated transformation efficiency.
 +
 
 +
|-
 +
! width="140" |Goals/Tasks for Next Meeting
 +
| We will learn about DNA ligation.
 +
|-
 +
! width="140" |Other Notes
 +
|  Although some students failed, they worked hard on the lab.
 +
|-
 +
|}
 +
 
 +
{{anchor|21mar2014}}
 +
{| class="wikitable" style="text-align:left; width:100%; height:200px;"
 +
|-
 +
!colspan="2" style="background: #ffdead;" |March 21th 2014
 +
|-
 +
! width="140" | Mission Statement
 +
| Learn about DNA Ligation
 +
|-
 +
! width="140" |Meeting Overview
 +
|  We received a lesson on DNA Ligation from Mr.Resch.
 +
|-
 +
|}
 +
 
 +
{{anchor|4apr2014}}
 +
{| class="wikitable" style="text-align:left; width:100%; height:200px;"
 +
|-
 +
!colspan="2" style="background: #ffdead;" |April 4th 2014
 +
|-
 +
! width="140" | Mission Statement
 +
| Continue research on project.
 +
|-
 +
! width="140" |Meeting Overview
 +
|  We continued researching appropriate promoters, ribosome binding sites, and terminators on the iGem registry.
 +
|-
 +
! width="140" |Goals/Tasks for Next Meeting
 +
| We will receive a lesson on mathematical modeling from Dr. Fishman.
 +
|-
 +
|}
 +
 
 +
{{anchor|11apr2014}}
 +
{| class="wikitable" style="text-align:left; width:100%; height:200px;"
 +
|-
 +
!colspan="2" style="background: #ffdead;" |April 11th 2014
 +
|-
 +
! width="140" | Mission Statement
 +
| We had a presentation of mathematical modeling by Dr.Fishman.
 +
|-
 +
! width="140" |Meeting Overview
 +
|  Dr. Fishman came to for an overview of mathematical modeling.
 +
R&D researched further for an enzyme that would break down fatty acids.
 +
 
 +
|-
 +
! width="140" |Goals/Tasks for Next Meeting
 +
| We hope to further understand mathematical modeling.
 +
|-
 +
|}
 +
 
 +
{{anchor|30may2014}}
 +
{| class="wikitable" style="text-align:left; width:100%; height:200px;"
 +
|-
 +
!colspan="2" style="background: #ffdead;" |May 30th 2014
 +
|-
 +
! width="140" | Mission Statement
 +
| Refine the Team Wiki and order the materials for our project.
 +
|-
 +
! width="140" |Meeting Overview
 +
|  Board Members and other juniors gathered to finalize the wiki page as well as take care of materials. We all continued researching promoters and ribosome binding sites for our project.
 +
|-
 +
! width="140" |Goals/Tasks for Next Meeting
 +
| Continue progressing through the steps needed to finalize the project and synthesize our desired protein.
|-
|-
|}
|}

Latest revision as of 19:55, 3 June 2014

Team Log

The Montgomery iGEM's teams documentation of activities, labs, and lectures for each day.

November 22nd 2013
Mission Statement We wanted to implement a new attendance sign-in setup, announce participation consent forms (principle consent form), and organize sub-teams.
Meeting Overview We started by electronically taking attendance and introducing the two sub-teams. All members were allowed to choose their sub-teams (may cut down R&D later). We talked about fundraising because of the December 31st deadline of registration and payment.

Possible fundraising ideas: - Hot chocolate - Snow-hat sales - ShopRite Bagging It was also the last “introductory” meeting. We announced the deadline for leadership applications due Wednesday, November 27 and reminded members to fill out consent forms.

Goals/Tasks for Next Meeting We planned to come up with an idea for fundraising and collect all the participation consent forms.

Finished principle consent form

Other Notes If members tried to look for sponsors, they had to send iGEM write-ups to them.

We found sponsors quickly and focused on small companies in Montgomery. In addition, we checked with the supervisor to see whether he had any ideas or grants.

December 13th 2013
Mission Statement We aimed to effectively organize the team and keep progressing with the fundraising and sponsorship efforts, as well as assemble the 2013-2014 board members.
Meeting Overview We began the meeting by announcing the 2013/2014 board members, followed by showing a video about synthetic biology to help members understand the importance of the team. The team will consist of the board as well as two sub-teams. The board collected emails and planned for fundraising and registration.
Goals/Tasks for Next Meeting We hoped to start teaching the basics of synthetic biology, organizing the ShopRite bagging fundraiser, creating online accounts for everyone, and cleaning up attendance lists.

December 20th 2013
Mission Statement We designed biological circuit from DNA sequence
Meeting Overview We received confirmation from ShopRite. The college advisor, Gavin from Purdue University came in to discuss synthetic biology and safety regulations. The R&D team learned how to use micropipettes while the operations discussed fundraiser ideas. .
Goals/Tasks for Next Meeting ShopRite bagging fundraiser

January 17th 2014
Mission Statement We learned information and became organized for the future.
Meeting Overview The meeting began with a presentation on synthetic biology. We discussed cutting down the R&D team, introduced plasmids and gene cloning, and explained the operations team structure. We also researched wiki websites.
Goals/Tasks for Next Meeting There were two goals: we went over the lab procedure for the pGLO lab and we established a bank account for Montgomery iGEM.
Other Notes We created a curriculum for the next few weeks, began discussing T-shirt designs and organized a board meeting at the Princeton Public Library on Sunday, 1/19/14.


January 24th 2014
Mission Statement Teach members about pGLO Lab.
Meeting Overview We explained how the iGEM google drive folders work and showed a bacterial transformation video. We ran a Q&A session. Each member had to demonstrate knowledge of the steps of the pGLO lab.
Goals/Tasks for Next Meeting We will understand the process of bacterial transformation and introduce laboratory techniques
Other Notes T-shirt design should be done by Feb 14th. Design winner gets free t-shirt

January 31th 2014
Mission Statement Go over lab technique.
Meeting Overview Aseptic technique, review pGLO lab.

How to clean with a Bunsen Burner(if metal use plastic loop).

Goals/Tasks for Next Meeting We accomplished the pGLO lab successfully without contamination and learned to make our own plasmids to put into bacteria.

February 3rd 2014
Mission Statement Continue preparations for pGLO Lab.
Meeting Overview We continued preparations for the pGLO lab. Members reviewed how the the lab procedure and also reviewed how the plasmid would be affected during the process.
Goals/Tasks for Next Meeting There was a complication with the materials which didn’t allow us to do the lab at the next meeting.

February 20th 2014
Mission Statement We changed the dynamics of iGEM and administered the lab practical.
Meeting Overview We collected t-shirt money, and Robert gave a speech on dynamics. There was a Q&A session about the lab practical and iGEM
Goals/Tasks for Next Meeting Prepare for pGLO lab
Other Notes Shoprite bagging in March

Need to take yearbook photo soon. Meetings are mandatory.

March 7th 2014
Mission Statement We finished the pGLO lab today. We organized the operations team.
Meeting Overview We formatted team organization, finalize acne project description, and administered the practice lab documentations. Board member headshots were also taken.
Goals/Tasks for Next Meeting We started DNA ligation lessons and researched more on the acne project.
Other Notes We started website structure.

March 14th 2014
Mission Statement We played an icebreaker to unify the team, went over lab results, and did post-lab calculations.
Meeting Overview We went over t-shirt sizes, reviewed team members' names and favorite Pokemon, went over results from pGLO lab, counted number of colonies growing under UV light, and calculated transformation efficiency.
Goals/Tasks for Next Meeting We will learn about DNA ligation.
Other Notes Although some students failed, they worked hard on the lab.


March 14th 2014
Mission Statement We played an icebreaker to unify the team, went over lab results, and did post-lab calculations.
Meeting Overview We went over t-shirt sizes, reviewed team members' names and favorite Pokemon, went over results from pGLO lab, counted number of colonies growing under UV light, and calculated transformation efficiency.
Goals/Tasks for Next Meeting We will learn about DNA ligation.
Other Notes Although some students failed, they worked hard on the lab.

March 21th 2014
Mission Statement Learn about DNA Ligation
Meeting Overview We received a lesson on DNA Ligation from Mr.Resch.

April 4th 2014
Mission Statement Continue research on project.
Meeting Overview We continued researching appropriate promoters, ribosome binding sites, and terminators on the iGem registry.
Goals/Tasks for Next Meeting We will receive a lesson on mathematical modeling from Dr. Fishman.

April 11th 2014
Mission Statement We had a presentation of mathematical modeling by Dr.Fishman.
Meeting Overview Dr. Fishman came to for an overview of mathematical modeling.

R&D researched further for an enzyme that would break down fatty acids.

Goals/Tasks for Next Meeting We hope to further understand mathematical modeling.

May 30th 2014
Mission Statement Refine the Team Wiki and order the materials for our project.
Meeting Overview Board Members and other juniors gathered to finalize the wiki page as well as take care of materials. We all continued researching promoters and ribosome binding sites for our project.
Goals/Tasks for Next Meeting Continue progressing through the steps needed to finalize the project and synthesize our desired protein.

Our Project

Extras